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Naslov:Loop-mediated isothermal amplification of specific endoglucanase gene sequence for detection of the bacterial wilt pathogen Ralstonia solanacearum
Avtorji:ID Lenarčič, Rok (Avtor)
ID Morisset, Dany (Avtor)
ID Pirc, Manca (Avtor)
ID Llop, Pablo (Avtor)
ID Ravnikar, Maja (Avtor)
ID Dreo, Tanja (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (339,81 KB)
MD5: 3C332C31F860C87CA71C09091416A330
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1371/journal.pone.0096027
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:The increased globalization of crops production and processing industries also promotes the side-effects of more rapid and efficient spread of plant pathogens. To prevent the associated economic losses, and particularly those related to bacterial diseases where their management relies on removal of the infected material from production, simple, easy-to-perform, rapid and cost-effective tests are needed. Loop-mediated isothermal amplification (LAMP) assays that target 16S rRNA, fliC and egl genes were compared and evaluated as on-site applications. The assay with the best performance was that targeted to the egl gene, which shows high analytical specificity for diverse strains of the betaproteobacterium Ralstonia solanacearum, including its non-European and non-race 3 biovar 2 strains. The additional melting curve analysis provides confirmation of the test results. According to our extensive assessment, the egl LAMP assay requires minimum sample preparation (a few minutes of boiling) for the identification of pure cultures and ooze from symptomatic material, and it can also be used in a high-throughput format in the laboratory. This provides sensitive and reliable detection of R. solanacearum strains of different phylotypes.
Ključne besede:plant pathogens, bacterial diseases
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:24.04.2014
Leto izida:2014
Št. strani:str. 1-8
Številčenje:Vol. 9, iss. 4
PID:20.500.12556/DiRROS-20003 Novo okno
UDK:581.2
ISSN pri članku:1932-6203
DOI:10.1371/journal.pone.0096027 Novo okno
COBISS.SI-ID:3114575 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 29. 4. 2014;
Datum objave v DiRROS:02.08.2024
Število ogledov:497
Število prenosov:218
Metapodatki:XML DC-XML DC-RDF
:
LENARČIČ, Rok, MORISSET, Dany, PIRC, Manca, LLOP, Pablo, RAVNIKAR, Maja in DREO, Tanja, 2014, Loop-mediated isothermal amplification of specific endoglucanase gene sequence for detection of the bacterial wilt pathogen Ralstonia solanacearum. PloS one [na spletu]. 2014. Vol. 9, no. 4, p. 1–8. [Dostopano 29 marec 2025]. DOI 10.1371/journal.pone.0096027. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=20003
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Gradivo je del revije

Naslov:PloS one
Založnik:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 Novo okno

Gradivo je financirano iz projekta

Financer:EC - European Commission
Številka projekta:245047
Naslov:Developing quarantine pest detection methods for use by national plant protection organizations (NPPO) and inspection services
Akronim:QDETECT
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:L1-3642
Naslov:Developing simple, rapid and on-site methods for plant pathogens detection

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

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