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Title:Imaging of human glioblastoma cells and their interactions with mesenchymal stem cells in the zebrafish (Danio rerio) embryonic brain
Authors:ID Vittori, Miloš (Author)
ID Breznik, Barbara (Author)
ID Gredar, Tajda (Author)
ID Hrovat, Katja (Author)
ID Bizjak-Mali, Lilijana (Author)
ID Lah Turnšek, Tamara (Author)
Files:.pdf PDF - Presentation file, download (1,35 MB)
MD5: 12976EB9DF7259336D9D59086B5DDB69
 
URL URL - Source URL, visit https://doi.org/10.1515/raon-2016-0017
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Background An attractive approach in the study of human cancers is the use of transparent zebrafish (Danio rerio) embryos, which enable the visualization of cancer progression in a living animal. Materials and methods We implanted mixtures of fluorescently labeled glioblastoma (GBM) cells and bonemarrow-derived mesenchymal stem cells (MSCs) into zebrafish embryos to study the cellular pathways of their invasion and the interactions between these cells in vivo. Results By developing and applying a carbocyanine-dye-compatible clearing protocol for observation of cells in deep tissues, we showed that U87 and U373 GBM cells rapidly aggregated into tumor masses in the ventricles and midbrain hemispheres of the zebrafish embryo brain, and invaded the central nervous system, often using the ventricular system and the central canal of the spinal cord. However, the GBM cells did not leave the central nervous system. With co-injection of differentially labeled cultured GBM cells and MSCs, the implanted cells formed mixed tumor masses in the brain. We observed tight associations between GBM cells and MSCs, and possible cell-fusion events. GBM cells and MSCs used similar invasion routes in the central nervous system. Conclusions This simple model can be used to study the molecular pathways of cellular processes in GBM cell invasion, and their interactions with various types of stromal cells in double or triple cell co-cultures, to design anti-GBM cell therapies that use MSCs as vectors.
Keywords:brain tumors, tumor microenvironment, animal models, xenotransplantation
Publication status:Published
Publication version:Version of Record
Publication date:01.06.2016
Year of publishing:2016
Number of pages:str. 159-167, III
Numbering:Vol. 50, no. 2
PID:20.500.12556/DiRROS-19849 New window
UDC:616-006
ISSN on article:1318-2099
DOI:10.1515/raon-2016-0017 New window
COBISS.SI-ID:3853391 New window
Publication date in DiRROS:25.07.2024
Views:307
Downloads:253
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Record is a part of a journal

Title:Radiology and oncology
Shortened title:Radiol. oncol.
Publisher:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0245-2015
Name:Ekotoksiologija, toksikološka genomika in karcinogeneza

Funder:EC - European Commission
Funding programme:INTERREG EC Project 2011
Acronym:GLIOMA

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:možganski tumorji, poskusi na živalih


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