20.500.12556/DiRROS-19849 Imaging of human glioblastoma cells and their interactions with mesenchymal stem cells in the zebrafish (Danio rerio) embryonic brain Background An attractive approach in the study of human cancers is the use of transparent zebrafish (Danio rerio) embryos, which enable the visualization of cancer progression in a living animal. Materials and methods We implanted mixtures of fluorescently labeled glioblastoma (GBM) cells and bonemarrow-derived mesenchymal stem cells (MSCs) into zebrafish embryos to study the cellular pathways of their invasion and the interactions between these cells in vivo. Results By developing and applying a carbocyanine-dye-compatible clearing protocol for observation of cells in deep tissues, we showed that U87 and U373 GBM cells rapidly aggregated into tumor masses in the ventricles and midbrain hemispheres of the zebrafish embryo brain, and invaded the central nervous system, often using the ventricular system and the central canal of the spinal cord. However, the GBM cells did not leave the central nervous system. With co-injection of differentially labeled cultured GBM cells and MSCs, the implanted cells formed mixed tumor masses in the brain. We observed tight associations between GBM cells and MSCs, and possible cell-fusion events. GBM cells and MSCs used similar invasion routes in the central nervous system. Conclusions This simple model can be used to study the molecular pathways of cellular processes in GBM cell invasion, and their interactions with various types of stromal cells in double or triple cell co-cultures, to design anti-GBM cell therapies that use MSCs as vectors. brain tumors tumor microenvironment animal models xenotransplantation možganski tumorji poskusi na živalih true false true Angleški jezik Slovenski jezik Neznano 2024-07-25 17:29:16 2024-07-25 17:29:17 2024-07-29 03:56:27 0000-00-00 00:00:00 2016 0 0 str. 159-167, III no. 2 Vol. 50 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2016-06-01 616-006 1318-2099 10.1515/raon-2016-0017 3853391 RAZ_Vittori_Milos_2016.pdf RAZ_Vittori_Milos_2016.pdf 1 12976EB9DF7259336D9D59086B5DDB69 a69cf37fbef040aeb260e801b9614a53853bc51b951a0b7fc29ce86b934a3b64 3f751f73-4a8a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27539 https://doi.org/10.1515/raon-2016-0017 0 532cd1b8-4a8a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27540 Nacionalni inštitut za biologijo 0 0 0