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Naslov:Imaging of human glioblastoma cells and their interactions with mesenchymal stem cells in the zebrafish (Danio rerio) embryonic brain
Avtorji:ID Vittori, Miloš (Avtor)
ID Breznik, Barbara (Avtor)
ID Gredar, Tajda (Avtor)
ID Hrovat, Katja (Avtor)
ID Bizjak-Mali, Lilijana (Avtor)
ID Lah Turnšek, Tamara (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (1,35 MB)
MD5: 12976EB9DF7259336D9D59086B5DDB69
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1515/raon-2016-0017
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Background An attractive approach in the study of human cancers is the use of transparent zebrafish (Danio rerio) embryos, which enable the visualization of cancer progression in a living animal. Materials and methods We implanted mixtures of fluorescently labeled glioblastoma (GBM) cells and bonemarrow-derived mesenchymal stem cells (MSCs) into zebrafish embryos to study the cellular pathways of their invasion and the interactions between these cells in vivo. Results By developing and applying a carbocyanine-dye-compatible clearing protocol for observation of cells in deep tissues, we showed that U87 and U373 GBM cells rapidly aggregated into tumor masses in the ventricles and midbrain hemispheres of the zebrafish embryo brain, and invaded the central nervous system, often using the ventricular system and the central canal of the spinal cord. However, the GBM cells did not leave the central nervous system. With co-injection of differentially labeled cultured GBM cells and MSCs, the implanted cells formed mixed tumor masses in the brain. We observed tight associations between GBM cells and MSCs, and possible cell-fusion events. GBM cells and MSCs used similar invasion routes in the central nervous system. Conclusions This simple model can be used to study the molecular pathways of cellular processes in GBM cell invasion, and their interactions with various types of stromal cells in double or triple cell co-cultures, to design anti-GBM cell therapies that use MSCs as vectors.
Ključne besede:brain tumors, tumor microenvironment, animal models, xenotransplantation
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.06.2016
Leto izida:2016
Št. strani:str. 159-167, III
Številčenje:Vol. 50, no. 2
PID:20.500.12556/DiRROS-19849 Novo okno
UDK:616-006
ISSN pri članku:1318-2099
DOI:10.1515/raon-2016-0017 Novo okno
COBISS.SI-ID:3853391 Novo okno
Datum objave v DiRROS:25.07.2024
Število ogledov:310
Število prenosov:254
Metapodatki:XML DC-XML DC-RDF
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Gradivo je del revije

Naslov:Radiology and oncology
Skrajšan naslov:Radiol. oncol.
Založnik:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P1-0245-2015
Naslov:Ekotoksiologija, toksikološka genomika in karcinogeneza

Financer:EC - European Commission
Program financ.:INTERREG EC Project 2011
Akronim:GLIOMA

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:možganski tumorji, poskusi na živalih


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