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Naslov:Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection
Avtorji:ID Dobnik, David (Avtor)
ID Štebih, Dejan (Avtor)
ID Blejec, Andrej (Avtor)
ID Morisset, Dany (Avtor)
ID Žel, Jana (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (709,65 KB)
MD5: 2C48F9F0225704E5DEACF52741B9A2CD
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1038/srep35451
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. Droplet digital PCR platforms, which include two fluorescence filters, support at least duplex reactions and with some developments and optimization higher multiplexing is possible. The present study not only shows a development of multiplex assays in droplet digital PCR, but also presents a first thorough evaluation of several parameters in such multiplex digital PCR. Two 4-plex assays were developed for quantification of 8 different DNA targets (7 genetically modified maize events and maize endogene). Per assay, two of the targets were labelled with one fluorophore and two with another. As current analysis software does not support analysis of more than duplex, a new R- and Shiny-based web application analysis tool (http://bit.ly/ddPCRmulti) was developed that automates the analysis of 4-plex results. In conclusion, the two developed multiplex assays are suitable for quantification of GMO maize events and the same approach can be used in any other field with a need for accurate and reliable quantification of multiple DNA targets.
Ključne besede:digital PCR, DNA targets, GMO detection
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:14.10.2016
Leto izida:2016
Št. strani:str. 1-9
Številčenje:Vol. 6
PID:20.500.12556/DiRROS-19787 Novo okno
UDK:577.2
ISSN pri članku:2045-2322
DOI:10.1038/srep35451 Novo okno
COBISS.SI-ID:4055119 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 18. 10. 2016;
Datum objave v DiRROS:25.07.2024
Število ogledov:516
Število prenosov:282
Metapodatki:XML DC-XML DC-RDF
:
DOBNIK, David, ŠTEBIH, Dejan, BLEJEC, Andrej, MORISSET, Dany in ŽEL, Jana, 2016, Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection. Scientific reports [na spletu]. 2016. Vol. 6, p. 1–9. [Dostopano 2 april 2025]. DOI 10.1038/srep35451. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19787
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Gradivo je del revije

Naslov:Scientific reports
Skrajšan naslov:Sci. rep.
Založnik:Nature Publishing Group
ISSN:2045-2322
COBISS.SI-ID:18727432 Novo okno

Gradivo je financirano iz projekta

Financer:EC - European Commission
Številka projekta:613908
Naslov:Development of Cost efficient Advanced DNA-based methods for specific Traceability issues and High Level On-site applicatioNs
Akronim:DECATHLON
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0165-2015
Naslov:Biotehnologija in sistemska biologija rastlin

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

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