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Naslov:Newly isolated bacteriophages from the Podoviridae, Siphoviridae, and Myoviridae families have variable effects on putative novel Dickeya spp.
Avtorji:ID Alič, Špela (Avtor)
ID Mikuletič, Tina (Avtor)
ID Tušek-Žnidarič, Magda (Avtor)
ID Ravnikar, Maja (Avtor)
ID Rački, Nejc (Avtor)
ID Peterka, Matjaž (Avtor)
ID Dreo, Tanja (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://www.frontiersin.org/articles/10.3389/fmicb.2017.01870/full
 
.pdf PDF - Predstavitvena datoteka, prenos (4,34 MB)
MD5: D31304A379C4D82A094E135741C0DE09
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Soft rot pathogenic bacteria from the genus Dickeya cause severe economic losses in orchid nurseries worldwide, and there is no effective control currently available. In the last decade, the genus Dickeya has undergone multiple changes as multiple new taxa have been described, and just recently a new putative Dickeya species was reported. This study reports the isolation of three bacteriophages active against putative novel Dickeya spp. isolates from commercially produced infected orchids that show variable host-range profiles. Bacteriophages were isolated through enrichment from Dickeya-infected orchid tissue. Convective interaction media monolith chromatography was used to isolate bacteriophages from wastewaters, demonstrating its suitability for the isolation of infective bacteriophages from natural sources. Based on bacteriophage morphology, all isolated bacteriophages were classified as being in the order Caudovirales, belonging to three different families, Podoviridae, Myoviridae, and Siphoviridae. The presence of three different groups of bacteriophages was confirmed by analyzing the bacteriophage specificity of bacterial hosts, restriction fragment length polymorphism and plaque morphology. Bacteriophage BF25/12, the first reported Podoviridae bacteriophage effective against Dickeya spp., was selected for further characterization. Its genome sequence determined by next-generation sequencing showed limited similarity to other characterized Podoviridae bacteriophages. Interactions among the bacteriophages and Dickeya spp. were examined using transmission electron microscopy, which revealed degradation of electron-dense granules in response to bacteriophage infection in some Dickeya strains. The temperature stability of the chosen Podoviridae bacteriophage monitored over 1 year showed a substantial decrease in the survival of bacteriophages stored at -20∘C over longer periods. It showed susceptibility to low pH and UV radiation but was stable in neutral and alkaline pH. Furthermore, the stability of the tested bacteriophage was also connected to the incubation medium and bacteriophage concentration at certain pH values. Finally, the emergence of bacteriophage-resistant bacterial colonies is highly connected to the concentration of bacteriophages in the bacterial environment. This is the first report on bacteriophages against Dickeya from the Podoviridae family to expand on potential bacteriophages to include in bacteriophage cocktails as biocontrol agents. Some of these bacteriophage isolates also showed activity against Dickeya solani, an aggressive strain that causes the soft rot of potatoes, which indicates their broad potential as biocontrol agents.
Ključne besede:bacteriophages, genome sequencing, resistance development, convective interaction media monolith chromatography
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:28.09.2017
Leto izida:2017
Št. strani:str. 1-14
Številčenje:Vol. 8
PID:20.500.12556/DiRROS-19716 Novo okno
UDK:577.2
ISSN pri članku:1664-302X
DOI:10.3389/fmicb.2017.01870 Novo okno
COBISS.SI-ID:4441679 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 28. 9. 2017;
Datum objave v DiRROS:25.07.2024
Število ogledov:499
Število prenosov:298
Metapodatki:XML DC-XML DC-RDF
:
ALIČ, Špela, MIKULETIČ, Tina, TUŠEK-ŽNIDARIČ, Magda, RAVNIKAR, Maja, RAČKI, Nejc, PETERKA, Matjaž in DREO, Tanja, 2017, Newly isolated bacteriophages from the Podoviridae, Siphoviridae, and Myoviridae families have variable effects on putative novel Dickeya spp. Frontiers in microbiology [na spletu]. 2017. Vol. 8, p. 1–14. [Dostopano 2 april 2025]. DOI 10.3389/fmicb.2017.01870. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19716
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Gradivo je del revije

Naslov:Frontiers in microbiology
Skrajšan naslov:Front. microbiol.
Založnik:Frontiers Research Foundation
ISSN:1664-302X
COBISS.SI-ID:4146296 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0165-2015
Naslov:Biotehnologija in sistemska biologija rastlin
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:L7-5534-2013
Naslov:RAZVOJ NOVIH TEHNOLOGIJ ZA DETEKCIJO, KVANTIFIKACIJO IN VREDNOTENJE BAKTERIOFAGOV
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:1000-15-0105
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Centre of Excellence forBiosensors, Instrumentation and Process Contro

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Latinski jezik
Ključne besede:Dickeya, Podoviridae


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