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Naslov:Mesenchymal stem cells differentially affect the invasion of distinct glioblastoma cell lines
Avtorji:ID Breznik, Barbara (Avtor)
ID Motaln, Helena (Avtor)
ID Vittori, Miloš (Avtor)
ID Rotter, Ana (Avtor)
ID Lah Turnšek, Tamara (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (15,25 MB)
MD5: 15A63F2D8D3B1C20349EFEB03EFEED26
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.18632/oncotarget.16041
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Glioblastoma multiforme are an aggressive form of brain tumors that are characterized by distinct invasion of single glioblastoma cells, which infiltrate the brain parenchyma. This appears to be stimulated by the communication between cancer and stromal cells. Mesenchymal stem cells (MSCs) are part of the glioblastoma microenvironment, and their ‘cross-talk’ with glioblastoma cells is still poorly understood. Here, we examined the effects of bone marrow-derived MSCs on two different established glioblastoma cell lines U87 and U373. We focused on mutual effects of direct MSC/glioblastoma contact on cellular invasion in three-dimensional invasion assays in vitro and in a zebrafish embryo model in vivo. This is the first demonstration of glioblastoma cell-type-specific responses to MSCs in direct glioblastoma co-cultures, where MSCs inhibited the invasion of U87 cells and enhanced the invasion of U373. Inversely, direct cross-talk between MSCs and both of glioblastoma cell lines enhanced MSC motility. MSC-enhanced invasion of U373 cells was assisted by overexpression of proteases cathepsin B, calpain1, uPA/uPAR, MMP-2, -9 and -14, and increased activities of some of these proteases, as determined by the effects of their selective inhibitors on invasion. In contrast, these proteases had no effect on U87 cell invasion under MSC co-culturing. Finally, we identified differentially expressed genes, in U87 and U373 cells that could explain different response of these cell lines to MSCs. In conclusion, we demonstrated that MSC/glioblastoma cross-talk is different in the two glioblastoma cell phenotypes, which contributes to tumor heterogeneity.
Ključne besede:glioblastoma multiforme, proteases, mesenchymal stem cells, tumor heterogeneity, zebrafish model
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:09.03.2017
Leto izida:2017
Št. strani:str. 25482-25499
Številčenje:Vol. 8, no. 15
PID:20.500.12556/DiRROS-19706 Novo okno
UDK:577.2
ISSN pri članku:1949-2553
DOI:10.18632/oncotarget.16041 Novo okno
COBISS.SI-ID:4238415 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 13. 2. 2017;
Datum objave v DiRROS:24.07.2024
Število ogledov:501
Število prenosov:304
Metapodatki:XML DC-XML DC-RDF
:
BREZNIK, Barbara, MOTALN, Helena, VITTORI, Miloš, ROTTER, Ana in LAH TURNŠEK, Tamara, 2017, Mesenchymal stem cells differentially affect the invasion of distinct glioblastoma cell lines. Oncotarget [na spletu]. 2017. Vol. 8, no. 15, p. 25482–25499. [Dostopano 2 april 2025]. DOI 10.18632/oncotarget.16041. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19706
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Gradivo je del revije

Naslov:Oncotarget
Skrajšan naslov:Oncotarget
Založnik:Impact Journals
ISSN:1949-2553
COBISS.SI-ID:3833969 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P1-0245-2015
Naslov:Ekotoksiologija, toksikološka genomika in karcinogeneza
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:J1-4274
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Naslov:Young Researcher Grant
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Operational Program for Italy-Slovenia, 2007–2013
Naslov:INTERREG project
Akronim:CB134-GLIOMA

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

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