| Title: | Diagnostic performance of p16/Ki-67 dual immunostaining at different number of positive cells in cervical smears in women referred for colposcopy |
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| Authors: | ID Salobir Gajšek, Uršula (Author)
ID Dovnik, Andraž (Author)
ID Takač, Iztok (Author)
ID Ivanuš, Urška (Author)
ID Jerman, Tine (Author)
ID Šramek Zatler, Simona (Author)
ID Repše-Fokter, Alenka (Author) |
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| Files: |  PDF - Presentation file, download (441,03 KB)
MD5: 41B9F7BC8F3B144133EDA11E722F9FB2
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  OI - Institute of Oncology
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| Abstract: | . The aim of the study was to evaluate the diagnostic accuracy of p16/Ki-67 dual immunostaining (p16/ Ki-67 DS) in cervical cytology and the number of positive p16/Ki-67 cells to diagnose high grade cervical intraepithelial neoplasia (CIN2+) in colposcopy population. Subjects and methods. We performed an analysis on a subset cohort of 174 women enrolled within a large-scale randomised controlled human papillomavirus (HPV) self-sampling project organised as part of the population-based Cervical Cancer Screening Programme ZORA in Slovenia. This subset cohort of patients was invited to the colposcopy clinic, underwent p16/Ki-67 DS cervical cytology and had the number of p16/Ki-67 positive cells determined. Results. Among analysed women, 42/174 (24.1%) had histologically confirmed CIN2+. The risk for CIN2+ was increasing with the number of positive cells (p < 0.001). The sensitivity of p16/Ki-67 DS for detection of CIN2+ was 88.1%, specificity was 65.2%, positive predictive value was 44.6% and negative predictive value was 94.5%. Conclusions. Dual p16/Ki-67 immunostaining for the detection of CIN2+ has shown high sensitivity and high negative predictive value in our study, which is comparable to available published data. The number of p16/Ki-67 positive cells was significantly associated with the probability of CIN2+ detection. We observed a statistically significant and clinically relevant increase in specificity if the cut-off for a positive test was shifted from one cell to three cells. |
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| Keywords: | cervical cytology, high-grade dysplasia, p16/Ki-67 immunostaining |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2021 |
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| Publisher: | Association of Radiology and Oncology |
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| Year of publishing: | 2021 |
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| Number of pages: | str. 426-432, IV |
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| Numbering: | Vol. 55, no. 4 |
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| Source: | Ljubljana |
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| PID: | 20.500.12556/DiRROS-19623  |
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| UDC: | 616-07 |
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| ISSN on article: | 1318-2099 |
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| DOI: | 10.2478/raon-2021-0043 |
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| COBISS.SI-ID: | 89445891 |
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| Copyright: | by Authors |
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| Note: | Soavtorji: Andraz Dovnik, Iztok Takac, Urska Ivanus, Tine Jerman, Simona Sramek Zatler, Alenka Repse Fokter;
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| Publication date in DiRROS: | 22.07.2024 |
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| Views: | 374 |
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| Downloads: | 125 |
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