20.500.12556/DiRROS-19623 Diagnostic performance of p16/Ki-67 dual immunostaining at different number of positive cells in cervical smears in women referred for colposcopy . The aim of the study was to evaluate the diagnostic accuracy of p16/Ki-67 dual immunostaining (p16/ Ki-67 DS) in cervical cytology and the number of positive p16/Ki-67 cells to diagnose high grade cervical intraepithelial neoplasia (CIN2+) in colposcopy population. Subjects and methods. We performed an analysis on a subset cohort of 174 women enrolled within a large-scale randomised controlled human papillomavirus (HPV) self-sampling project organised as part of the population-based Cervical Cancer Screening Programme ZORA in Slovenia. This subset cohort of patients was invited to the colposcopy clinic, underwent p16/Ki-67 DS cervical cytology and had the number of p16/Ki-67 positive cells determined. Results. Among analysed women, 42/174 (24.1%) had histologically confirmed CIN2+. The risk for CIN2+ was increasing with the number of positive cells (p < 0.001). The sensitivity of p16/Ki-67 DS for detection of CIN2+ was 88.1%, specificity was 65.2%, positive predictive value was 44.6% and negative predictive value was 94.5%. Conclusions. Dual p16/Ki-67 immunostaining for the detection of CIN2+ has shown high sensitivity and high negative predictive value in our study, which is comparable to available published data. The number of p16/Ki-67 positive cells was significantly associated with the probability of CIN2+ detection. We observed a statistically significant and clinically relevant increase in specificity if the cut-off for a positive test was shifted from one cell to three cells. cervical cytology high-grade dysplasia p16/Ki-67 immunostaining true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-07-22 15:12:33 2024-07-22 15:12:33 2024-07-23 03:55:57 0000-00-00 00:00:00 2021 0 0 Soavtorji: Andraz Dovnik, Iztok Takac, Urska Ivanus, Tine Jerman, Simona Sramek Zatler, Alenka Repse Fokter; str. 426-432, IV no. 4 Vol. 55 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-01-01 616-07 1318-2099 10.2478/raon-2021-0043 89445891 RAZ_Salobir_Gajsek_Ursula_2021.pdf RAZ_Salobir_Gajsek_Ursula_2021.pdf 1 41B9F7BC8F3B144133EDA11E722F9FB2 3b1c6e7a1dcd891ffc3cfebaffc0821026d1a45f47cc54b37fb5dc8470ebff22 73045949-481b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27127 Onkološki inštitut Ljubljana 0 0 0