| Naslov: | Validating the potential of double-stranded RNA targeting Colorado potato beetle mesh gene in laboratory and field trials |
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| Avtorji: | ID Petek, Marko (Avtor)
ID Coll Rius, Anna (Avtor)
ID Ferenc, Rok (Avtor)
ID Razinger, Jaka (Avtor)
ID Gruden, Kristina (Avtor) |
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| Datoteke: |  PDF - Predstavitvena datoteka, prenos (438,63 KB)
MD5: CCB4911D13648CB638D5FF2AB7176212
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
 KIS - Kmetijski inštitut Slovenije
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| Povzetek: | Colorado potato beetle (CPB) is an agricultural pest of solanaceous crops, notorious for its rapid resistance development to chemical pesticides. Foliar spraying of dsRNA formulations is a promising innovative technology providing highly specific and environmentally acceptable option for CPB management. We designed dsRNA to silence CPB mesh gene (dsMESH) and performed laboratory feeding trials to assess impacts on beetle survival and development. We compared the effectiveness of in vivo and in vitro produced dsRNA in a series of laboratory experiments. We additionally performed a field trial in which the efficacy of dsRNA sprayed onto potato foliage was compared to a spinosad-based insecticide. We showed that dsMESH ingestion consistently and significantly impaired larval growth and decreased larval survival in laboratory feeding experiments. In vivo produced dsRNA performed similarly as in vitro synthesized dsRNA in laboratory settings. In the field trial, dsMESH was as effective in controlling CPB larvae as a commercial spinosad insecticide, its activity was however slower. We discuss limitations and benefits of a potential dsMESH-based CPB management strategy and list some important RNAi based CPB research topics, which will have to be addressed in future. |
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| Ključne besede: | RNA interference, RNA interference feeding, ds RNA, gene silencing, RNAi pest control, survival analysis, field trial |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 19.08.2020 |
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| Leto izida: | 2020 |
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| Št. strani: | Str. 1-9 |
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| Številčenje: | Vol. 11, article no.ǂ1250 |
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| PID: | 20.500.12556/DiRROS-19523  |
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| UDK: | 632 |
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| ISSN pri članku: | 1664-462X |
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| DOI: | 10.3389/fpls.2020.01250 |
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| COBISS.SI-ID: | 25419523 |
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| Opomba: | Nasl. iz nasl. zaslona;
Opis vira z dne 18.8.2020;
ǂarticle no.ǂ1250;
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| Datum objave v DiRROS: | 22.07.2024 |
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| Število ogledov: | 297 |
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| Število prenosov: | 118 |
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| Metapodatki: |    |
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