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Naslov:Feasibility of droplet digital PCR analysis of plasma cell-free DNA from kidney transplant patients
Avtorji:ID Jerič Kokelj, Barbara (Avtor)
ID Štalekar, Maja (Avtor)
ID Vencken, Sebastian (Avtor)
ID Dobnik, David (Avtor)
ID Kogovšek, Polona (Avtor)
ID Stanonik, Matjaž (Avtor)
ID Arnol, Miha (Avtor)
ID Ravnikar, Maja (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://www.frontiersin.org/articles/10.3389/fmed.2021.748668/full
 
.pdf PDF - Predstavitvena datoteka, prenos (842,05 KB)
MD5: 7FE0006D58E14DFED953376D087E1AAB
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo NIB - Nacionalni inštitut za biologijo
Povzetek:Increasing research demonstrates the potential of donor-derived cell-free DNA (dd-cfDNA) as a biomarker for monitoring the health of various solid organ transplants. Several methods have been proposed for cfDNA analysis, including real-time PCR, digital PCR, and next generation sequencing-based approaches. We sought to revise the droplet digital PCR (ddPCR)-based approach to quantify relative dd-cfDNA in plasma from kidney transplant (KTx) patients using a novel pilot set of assays targeting single nucleotide polymorphisms that have a very high potential to distinguish cfDNA from two individuals. The assays are capable of accurate quantification of down to 0.1% minor allele content when analyzing 165 ng of human DNA. We found no significant differences in the yield of extracted cfDNA using the three different commercial kits tested. More cfDNA was extracted from the plasma of KTx patients than from healthy volunteers, especially early after transplantation. The median level of donor-derived minor alleles in KTx samples was 0.35%. We found that ddPCR using the evaluated assays within specific range is suitable for analysis of KTx patientsʼ plasma but recommend prior genotyping of donor DNA and performing reliable preamplification of cfDNA.
Ključne besede:kidney transplantation, droplet digital PCR, plasma cell-free DNA, minor allele quantification, assay evaluation, graft health monitoring
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:08.10.2021
Leto izida:2021
Št. strani:str. 1-9
Številčenje:Vol. 8
PID:20.500.12556/DiRROS-19480 Novo okno
UDK:616.6:615
ISSN pri članku:2296-858X
DOI:10.3389/fmed.2021.748668 Novo okno
COBISS.SI-ID:79997955 Novo okno
Opomba:Soavtorji: Maja Štalekar, Sebastian Vencken, David Dobnik, Polona Kogovšek, Matjaž Stanonik, Miha Arnol and Maja Ravnikar; Nasl. z nasl. zaslona; Opis vira z dne 11. 10. 2021; Št. članka: 748668;
Datum objave v DiRROS:19.07.2024
Število ogledov:717
Število prenosov:282
Metapodatki:XML DC-XML DC-RDF
:
JERIČ KOKELJ, Barbara, ŠTALEKAR, Maja, VENCKEN, Sebastian, DOBNIK, David, KOGOVŠEK, Polona, STANONIK, Matjaž, ARNOL, Miha in RAVNIKAR, Maja, 2021, Feasibility of droplet digital PCR analysis of plasma cell-free DNA from kidney transplant patients. Frontiers in medicine [na spletu]. 2021. Vol. 8, p. 1–9. [Dostopano 21 april 2025]. DOI 10.3389/fmed.2021.748668. Pridobljeno s: https://dirros.openscience.si/IzpisGradiva.php?lang=slv&id=19480
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Gradivo je del revije

Naslov:Frontiers in medicine
Skrajšan naslov:Front. med.
Založnik:Frontiers Media S.A.
ISSN:2296-858X
COBISS.SI-ID:523095065 Novo okno

Gradivo je financirano iz projekta

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0165-2015
Naslov:Biotehnologija in sistemska biologija rastlin
Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P4-0407-2019
Naslov:Okoljska in aplikativna virologija: virusi, prijatelji in sovražniki
Financer:Drugi - Drug financer ali več financerjev
Program financ.:Ministry of Education, Science and Sport of the Republic of Slovenia
Številka projekta:C3330-17-529023
Naslov:Raziskovalci-2.0-NIB-529023

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

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