| Naslov: | Fast assay to predict multipotent mesenchymal stromal cell replicative senescence dynamics |
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| Avtorji: | ID Kološa, Katja (Avtor)
ID Leskovšek, Aleš (Avtor)
ID Rajar, Teja (Avtor)
ID Lah Turnšek, Tamara (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://www.future-science.com/doi/10.2144/btn-2021-0087
 PDF - Predstavitvena datoteka, prenos (1,19 MB)
MD5: 0B81386B969524176A30F380A871DE12
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
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| Povzetek: | The major obstacle to the application of mesenchymal stromal cells (MSCs) in regenerative medicine is the expansion of the donor-derived cells in vitro to obtain high cell numbers in the shortest possible time. However, MSCs gradually undergo replicative senescence after a variable number of divisions that reduce their therapeutic efficacy, which needs to be determined before administration. The authors developed a fast and simple evaluation assay testing two senescence inducers, mitoxantrone (Mxt) and trichostatin A (TSA), to predict the onset of spontaneous replicative senescence of adipose-derived mesenchymal stromal cells (ASCs) and have confirmed the correlation between induced senescence and spontaneous replicative senescence in the assay using Mxt. This protocol facilitates the standardization of therapeutic ASCs and MSCs from other origins before application. |
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| Ključne besede: | adipose mesenchymal stromal (stem) cells (ASCs), cell longevity, induced senescence, mesenchymal stromal (stem) cells (MSCs), mitoxantrone, replicative senescence, trichostatin A |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 17.02.2022 |
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| Leto izida: | 2022 |
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| Št. strani: | str. 1-10 |
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| Številčenje: | Vol. 72, no. 3 |
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| PID: | 20.500.12556/DiRROS-19343  |
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| UDK: | 577.2 |
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| ISSN pri članku: | 0736-6205 |
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| DOI: | 10.2144/btn-2021-0087 |
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| COBISS.SI-ID: | 100663043 |
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| Datum objave v DiRROS: | 16.07.2024 |
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| Število ogledov: | 397 |
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| Število prenosov: | 178 |
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| Metapodatki: |    |
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