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Naslov:Effects of desiccation and freezing on microbial ionizing radiation survivability : considerations for Mars sample return
Avtorji:ID Horne, William H. (Avtor)
ID Grebenc, Tine (Avtor)
ID Tkavc, Rok (Avtor)
ID Gostinčar, Cene (Avtor)
ID Gunde-Cimerman, Nina (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://www.liebertpub.com/doi/10.1089/ast.2022.0065
 
.pdf PDF - Predstavitvena datoteka, prenos (922,44 KB)
MD5: 49620760C9F60896644466FA840C161F
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo SciVie - Gozdarski inštitut Slovenije
Povzetek:Increasingly, national space agencies are expanding their goals to include Mars exploration with sample return. To better protect Earth and its biosphere from potential extraterrestrial sources of contamination, as set forth in the Outer Space Treaty of 1967, international efforts to develop planetary protection measures strive to understand the danger of cross-contamination processes in Mars sample return missions. We aim to better understand the impact of the martian surface on microbial dormancy and survivability. Radiation resistance of microbes is a key parameter in considering survivability of microbes over geologic times on the frigid, arid surface of Mars that is bombarded by solar and galactic cosmic radiation. We tested the influence of desiccation and freezing on the ionizing radiation survival of six model microorganisms: vegetative cells of two bacteria (Deinococcus radiodurans, Escherichia coli) and a strain of budding yeast (Saccharomyces cerevisiae); and vegetative cells and endospores of three Bacillus bacteria (B. subtilis, B. megaterium, B. thuringiensis). Desiccation and freezing greatly increased radiation survival of vegetative polyploid microorganisms when applied separately, and when combined, desiccation and freezing increased radiation survival even more so. Thus, the radiation survival threshold of polyploid D. radiodurans cells can be extended from the already high value of 25 kGy in liquid culture to an astonishing 140 kGy when the cells are both desiccated and frozen. However, such synergistic radioprotective effects of desiccation and freezing were not observed in monogenomic or digenomic Bacillus cells and endospores, which are generally sterilized by 12 kGy. This difference is associated with a critical requirement for survivability under radiation, that is, repair of genome damage caused by radiation. Deinococcus radiodurans and S. cerevisiae accumulate similarly high levels of the Mn antioxidants that are required for extreme radiation resistance, as do endospores, though they greatly exceed spores in radioresistance because they contain multiple identical genome copies, which in D. radiodurans are joined by persistent Holliday junctions. We estimate ionizing radiation survival limits of polyploid DNA-based life-forms to be hundreds of millions of years of background radiation while buried in the martian subsurface. Our findings imply that forward contamination of Mars will essentially be permanent, and backward contamination is a possibility if life ever existed on Mars.
Ključne besede:ionizing radiation, life on Mars, astrobiology, radiation, fungi, bacteria, radiotolerance
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Datum objave:01.01.2022
Leto izida:2022
Št. strani:str. 1337-1350
Številčenje:Vol. 22, no. 11
PID:20.500.12556/DiRROS-15699 Novo okno
UDK:579:523.43
ISSN pri članku:1531-1074
DOI:10.1089/ast.2022.0065 Novo okno
COBISS.SI-ID:127593987 Novo okno
Datum objave v DiRROS:03.11.2022
Število ogledov:559
Število prenosov:268
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Astrobiology
Založnik:Mary Ann Liebert
ISSN:1531-1074
COBISS.SI-ID:517339673 Novo okno

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:J4-2549-2020
Naslov:Razgradnja plastike s poliekstremotolerantnimi glivami

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:J4-3098-2021
Naslov:Izcedne talne vode kot neizkoriščen vir informacij o biotski raznovrstnosti tal

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P1-0198-2018
Naslov:Molekularno-biološke raziskave mikroorganizmov

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0432-2022
Naslov:Morska in mikrobna biotehnologija

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0107-2020
Naslov:Gozdna biologija, ekologija in tehnologija

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:03.11.2022

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:ekstremotoleranca, ionizirajoče sevanje, bakterije, glive, radiacija, astrobiologija, Mars, življenje na drugih planetih, življenje na Marsu


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