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Naslov:Evaluation of a novel plasmid for simultaneous gene electrotransfer-mediated silencing of CD105 and CD146 in combination with irradiation
Avtorji:ID Savarin, Monika (Avtor)
ID Kamenšek, Urška (Avtor)
ID Žnidar, Katarina (Avtor)
ID Todorović, Vesna (Avtor)
ID Serša, Gregor (Avtor)
ID Čemažar, Maja (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://www.mdpi.com/journal/ijms
 
.pdf PDF - Predstavitvena datoteka, prenos (4,74 MB)
MD5: 1AA6AAB5410529ADAF159488E9F205DE
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo OI - Onkološki inštitut Ljubljana
Povzetek:Targeting tumor vasculature through specific endothelial cell markers represents a promising approach for cancer treatment. Here our aim was to construct an antibiotic resistance gene-free plasmid encoding shRNAs to simultaneously target two endothelial cell markers, CD105 and CD146, and to test its functionality and therapeutic potential in vitro when delivered by gene electrotransfer (GET) and combined with irradiation (IR). Functionality of the plasmid was evaluated by determining the silencing of the targeted genes using qRT-PCR. Antiproliferative and antiangiogenic effects were determined by the cytotoxicity assay tube formation assay and wound healing assay in murine endothelial cells 2H-11. The functionality of the plasmid construct was also evaluated in malignant melanoma tumor cell line B16F10. Additionally, potential activation of immune response was measured by induction of DNA sensor STING and proinflammatory cytokines by qRT-PCR in endothelial cells 2H-11. We demonstrated that the plasmid construction was successful and can efficiently silence the expression of the two targeted genes. As a consequence of silencing, reduced migration rate and angiogenic potential was confirmed in 2H-11 endothelial cells. Furthermore, induction of DNA sensor STING and proinflammatory cytokines were determined, which could add to the therapeutic effectiveness when used in vivo. To conclude, we successfully constructed a novel plasmid DNA with two shRNAs, which holds a great promise for further in vivo testing.
Ključne besede:CD105, CD146, plasmid, gene electrotransfer
Leto izida:2021
Status objave pri reviji:Objavljeno
Verzija članka:Založnikova različica članka
Datum objave:17.03.2021
Založnik:MDPI
Št. strani:str. 3069-1-3069-18
Številčenje:Vol. 22
PID:20.500.12556/DiRROS-15568 Novo okno
UDK:602.6/.7
ISSN pri članku:1422-0067
DOI:10.3390/ijms22063069 Novo okno
COBISS.SI-ID:56427011 Novo okno
Avtorske pravice:by Authors
Opomba:Opis vira z dne 19. 3. 2021; Nasl. z nasl. zaslona;
Datum objave v DiRROS:21.09.2022
Število ogledov:38
Število prenosov:19
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:International journal of molecular sciences
Skrajšan naslov:Int. j. mol. sci.
Založnik:MDPI
ISSN:1422-0067
COBISS.SI-ID:2779162 Novo okno

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:Javna agencija Republike Slovenije za raziskovalno dejavnost
Številka projekta:Z3-1873-2019
Naslov:Nov pristop žilno ciljane terapije, kjer z gensko terapijo utišamo dve neodvisni signalni poti,v kombinaciji z radioterapijo

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:Javna agencija Republike Slovenije za raziskovalno dejavnost
Številka projekta:P3-0003-2015
Naslov:Razvoj in ovrednotenje novih terapij za zdravljenje malignih tumorjev

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:21.09.2022

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:CD105, CD146, plazmidi, elektroprenos genov


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