Digitalni repozitorij raziskovalnih organizacij Slovenije

Izpis gradiva
A+ | A- | SLO | ENG

Naslov:Neuromuscular junction instability and altered intracellular calcium handling as early determinants of force loss during unloading in humans
Avtorji:ID Monti, Elena (Avtor)
ID Reggiani, Carlo (Avtor)
ID Franchi, Martino V. (Avtor)
ID Toniolo, Luana (Avtor)
ID Sandri, Marco (Avtor)
ID Armani, Andrea (Avtor)
ID Zampieri, Sandra (Avtor)
ID Šimunič, Boštjan (Avtor)
ID Pišot, Rado (Avtor)
ID Narici, Marco Vincenzo (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (3,39 MB)
MD5: 6F943C20D4E806A13232B11641601867
Opis: This is an open access article under the terms of the Creative Commons Attribution-Non Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
 
URL URL - Izvorni URL, za dostop obiščite https://physoc.onlinelibrary.wiley.com/doi/10.1113/JP281365
 
URL URL - Izvorni URL, za dostop obiščite https://doi.org/10.1113/JP281365
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo ZRS Koper - Znanstveno-raziskovalno središče Koper / Centro di Ricerche Scientifiche Capodistria
Povzetek:Unloading induces rapid skeletal muscle atrophy and functional decline. Importantly, force is lost at a much higher rate than muscle mass. We aimed to investigate the early determinants of the disproportionate loss of force compared to that of muscle mass in response to unloading. Ten young participants underwent 10 days of bed rest (BR). At baseline (BR0) and at 10 days (BR10), quadriceps femoris (QF) volume (VOL) and isometric maximum voluntary contraction (MVC) were assessed. At BR0 and BR10 blood samples and biopsies of vastus lateralis (VL) muscle were collected. Neuromuscular junction (NMJ) stability and myofibre innervation status were assessed, together with single fibre mechanical properties and sarcoplasmic reticulum (SR) calcium handling. From BR0 to BR10, QFVOL and MVC decreased by 5.2% (P = 0.003) and 14.3% (P < 0.001), respectively. Initial and partial denervation was detected from increased neural cell adhesion molecule (NCAM)-positive myofibres at BR10 compared with BR0 (+3.4%, P = 0.016). NMJ instability was further inferred from increased C-terminal agrin fragment concentration in serum (+19.2% at BR10, P = 0.031). Fast fibre cross-sectional area (CSA) showed a trend to decrease by 15% (P = 0.055) at BR10, while single fibre maximal tension (force/CSA) was unchanged. However, at BR10 SR Ca2+ release in response to caffeine decreased by 35.1% (P < 0.002) and 30.2% (P < 0.001) in fast and slow fibres, respectively, pointing to an impaired excitation%contraction coupling. These findings support the view that the early onset of NMJ instability and impairment in SR function are eligible mechanisms contributing to the greater decline in muscle force than in muscle size during unloading.
Ključne besede:Ca2+ dynamics, muscle atrophy, neuromuscular junction instability, sarcoplasmic reticulum, single fibre atrophy, single fibre contractile impairment, unloading
Leto izida:2021
Status objave pri reviji:Objavljeno
Verzija članka:Založnikova različica članka
Št. strani:str. 3037-3061
Številčenje:Vol. 599 , iss. 12
UDK:612.74
ISSN pri članku:1469-7793
DOI:10.1113/JP281365 Novo okno
COBISS.SI-ID:64735747 Novo okno
Avtorske pravice:© 2021 The Authors
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 26. 5. 2021; Soavtorji: Elena Monti, Carlo Reggiani, Martino V. Franchi, Luana Toniolo, Marco Sandri, Andrea Armani, Sandra Zampieri, Emiliana Giacomello, Fabio Sarto, Giuseppe Sirago, Marta Murgia, Leonardo Nogara, Lorenzo Marcucci, Stefano Ciciliot, Boštjan Šimunic, Rado Pišot and Marco V. Narici;
Datum objave v DiRROS:16.06.2021
Število ogledov:555
Število prenosov:532
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
:
  
Objavi na:AddThis
AddThis uporablja piškotke, za katere potrebujemo vaše privoljenje.
Uredi privoljenje...

Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše podrobnosti ali sproži prenos.

Gradivo je del revije

Naslov:The journal of physiology
Skrajšan naslov:J. physiol.
Založnik:Cambridge University Press
ISSN:1469-7793
COBISS.SI-ID:515030553 Novo okno

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:15.06.2021

Sekundarni jezik

Jezik:Ni določen
Ključne besede:Ca2+ dinamika, mišičnoskeletni sistem, mišice, mišična atrofija, sarkoplazemski retikulum


Nazaj