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Title:Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathon
Authors:ID Jan, Zala (Author)
ID Drab, Mitja (Author)
ID Drobne, Damjana (Author)
ID Bedina Zavec, Apolonija (Author)
ID Benčina, Mojca (Author)
ID Drašler, Barbara (Author)
ID Hočevar, Matej (Author)
ID Krek, Judita Lea (Author)
ID Pađen, Ljubiša (Author)
ID Pajnič, Manca (Author)
ID Repar, Neža (Author)
ID Šimunič, Boštjan (Author)
ID Štukelj, Roman (Author)
ID Kralj-Iglič, Veronika (Author)
Files:.pdf PDF - Presentation file, download (1,88 MB)
MD5: 15BA5FE84BDD421A0549244AB94233B7
 
URL URL - Source URL, visit https://doi.org/10.2147/IJN.S282200
 
URL URL - Source URL, visit https://www.dovepress.com/decrease-in-cellular-nanovesicles-concentration-in-blood-of-athletes-m-peer-reviewed-article-IJN
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo ZRS Koper - Science and Research Centre Koper
Abstract:Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.
Keywords:membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon
Publication status:Published
Publication version:Version of Record
Year of publishing:2021
Number of pages:str. 443-456
Numbering:Vol. 16
PID:20.500.12556/DiRROS-13691 New window
UDC:577.1:796.422.16
ISSN on article:1178-2013
DOI:10.2147/IJN.S282200 New window
COBISS.SI-ID:48113411 New window
Copyright:© 2021 Jan et al.
Note:Nasl. z nasl. zaslona; Opis vira z dne 21. 1. 2021; Soavtorji: Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drasler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič;
Publication date in DiRROS:25.01.2021
Views:1243
Downloads:1014
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Record is a part of a journal

Title:International journal of nanomedicine
Publisher:Dove Medical Press
ISSN:1178-2013
COBISS.SI-ID:3223828 New window

Licences

License:CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
Link:http://creativecommons.org/licenses/by-nc/4.0/
Description:A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.
Licensing start date:20.01.2021

Secondary language

Language:Undetermined
Keywords:vesikulacija membrane, fizični napor, krvni vzorci, vnetni procesi, celični nano vezikli, maraton


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