20.500.12556/DiRROS-13691 Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathon Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds. membrane vesiculation physical effort blood samples inflammation process cellular nanovesicles marathon vesikulacija membrane fizični napor krvni vzorci vnetni procesi celični nano vezikli maraton true false true Angleški jezik Ni določen © 2021 Jan et al. Neznano 2021-01-25 09:06:34 2021-01-25 09:06:36 2022-08-19 03:36:06 0000-00-00 00:00:00 2021 0 0 Nasl. z nasl. zaslona; Opis vira z dne 21. 1. 2021; Soavtorji: Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drasler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič; str. 443-456 Vol. 16 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.1:796.422.16 1178-2013 10.2147/IJN.S282200 48113411 3223828 ZRS_Jan_Zala_i2021.pdf ZRS_Jan_Zala_i2021.pdf 1 15BA5FE84BDD421A0549244AB94233B7 ad56bfcfce6908dee554bc580c105fc29a47f7ab2e28d65509125e03da436552 716a7210-17b6-11ed-b6b8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=16719 https://www.dovepress.com/decrease-in-cellular-nanovesicles-concentration-in-blood-of-athletes-m-peer-reviewed-article-IJN 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=16718 https://doi.org/10.2147/IJN.S282200 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=20221 Znanstveno-raziskovalno središče Koper / Centro di Ricerche Scientifiche Capodistria 0 0 0