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Title:Chemokines during anaphylaxis : the importance of CCL2 and CCL2-dependent chemotactic activity for basophils
Authors:Vantur, Romana (Author)
Rihar, Maruša (Author)
Koren, Ana (Author)
Rijavec, Matija (Author)
Kopač, Peter (Author)
Bidovec, Urška (Author)
Eržen, Renato (Author)
Korošec, Peter (Author)
Language:English
Tipology:1.01 - Original Scientific Article
Organisation:Logo UKPBAG - University Clinic of Respiratory and Allergic Diseases Golnik
Abstract:Background: The role of chemokines in anaphylaxis is unclear. Methods: We prospectively recruited 49 patients presenting to the emergency department with an acute episode of anaphylaxis and 28 healthy subjects. We measured serum levels of the chemokines CCL2, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL21, CCL22, CCL24, and CCL26, tryptase, the absolute number of circulating basophils, monocytes, lymphocytes, and PMNs, and whole blood FCER1A, CPA3 and HDC gene expression at two time points: during the anaphylactic episode and in convalescent samples collected approximately 3 months later. We then investigated the in vitro chemotactic activity of chemokines induced during anaphylaxis for the in vitro migration of the corresponding cells. Results: Only CCL2 chemokine levels were signifcantly increased in anaphylaxis samples (median 514 pg/ml) compared to convalescent samples (284 pg/ml, P<0.0001) and healthy subjects (279 pg/ml, P<0.0001); there was no signifcant diference in any of the other chemokines. There was a signifcant positive correlation between the rates of increase of serum CCL2 (median [range]: 106.0% [-44.7% to 557.4%]) and tryptase (133.8% [-6.6% to 893.4%]; r=0.68, P<0.0001) and between the acute concentration of serum CCL2 and the acute concentration of serum tryptase (r=0.77, P<0.0001). The number of circulating basophils, but not other blood cells, signifcantly decreased during anaphylaxis (median 5.0 vs. 19.1 cells/[micro]l in convalescent samples; P<0.0001); a decrease in whole-blood gene expression of basophil markers (P
Keywords:anaphylaxis, chemokines, tryptases, basophils, chemotaxis, CCL2, cell migration
Year of publishing:2020
Publisher:Springer Nature
Source:Velika Britanija
COBISS_ID:43048963 Link is opened in a new window
UDC:616-097
ISSN on article:2045-7022
OceCobissID:31507929 Link is opened in a new window
DOI:10.1186/s13601-020-00367-2 Link is opened in a new window
Note:Nasl. z nasl. zaslona; Soavtorji: Maruša Rihar, Ana Koren, Matija Rijavec, Peter Kopač, Urška Bidovec-Stojković, Renato Eržen, Peter Korošec; Opis vira z dne 16. 12. 2020; Št. članka: 63;
Views:732
Downloads:299
Files:.pdf PDF - Presentation file, download (2,04 MB)
 
Journal:Clinical and translational allergy
BioMed Central
 
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Rights:© The Author(s) 2020
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License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:15.12.2020

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