20.500.12556/DiRROS-13682 Chemokines during anaphylaxis : the importance of CCL2 and CCL2-dependent chemotactic activity for basophils Background: The role of chemokines in anaphylaxis is unclear. Methods: We prospectively recruited 49 patients presenting to the emergency department with an acute episode of anaphylaxis and 28 healthy subjects. We measured serum levels of the chemokines CCL2, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL21, CCL22, CCL24, and CCL26, tryptase, the absolute number of circulating basophils, monocytes, lymphocytes, and PMNs, and whole blood FCER1A, CPA3 and HDC gene expression at two time points: during the anaphylactic episode and in convalescent samples collected approximately 3 months later. We then investigated the in vitro chemotactic activity of chemokines induced during anaphylaxis for the in vitro migration of the corresponding cells. Results: Only CCL2 chemokine levels were signifcantly increased in anaphylaxis samples (median 514 pg/ml) compared to convalescent samples (284 pg/ml, P<0.0001) and healthy subjects (279 pg/ml, P<0.0001); there was no signifcant diference in any of the other chemokines. There was a signifcant positive correlation between the rates of increase of serum CCL2 (median [range]: 106.0% [-44.7% to 557.4%]) and tryptase (133.8% [-6.6% to 893.4%]; r=0.68, P<0.0001) and between the acute concentration of serum CCL2 and the acute concentration of serum tryptase (r=0.77, P<0.0001). The number of circulating basophils, but not other blood cells, signifcantly decreased during anaphylaxis (median 5.0 vs. 19.1 cells/[micro]l in convalescent samples; P<0.0001); a decrease in whole-blood gene expression of basophil markers (P</=0.0018) confirmed these changes. Anaphylactic serum enhances the in vitro migration of basophils via CCL2-dependent chemotactic activity; in contrast, no CCL2-dependent chemotactic activity was observed for convalescent samples. Conclusions: Our findings imply an important and specifc role for CCL2-mediated chemotactic activity in the pathophysiology of human anaphylaxis. anaphylaxis chemokines tryptases basophils chemotaxis CCL2 cell migration anafilaksija kemokini triptaze bazofilci kemotaksa CCL2 celična migracija true false true Springer Nature Angleški jezik Ni določen © The Author(s) 2020 Neznano 2021-01-18 12:03:04 2021-01-18 12:03:05 2022-08-19 03:36:02 0000-00-00 00:00:00 2020 0 Velika Britanija 0 Nasl. z nasl. zaslona; Soavtorji: Maruša Rihar, Ana Koren, Matija Rijavec, Peter Kopač, Urška Bidovec-Stojković, Renato Eržen, Peter Korošec; Opis vira z dne 16. 12. 2020; Št. članka: 63; str. 1-11 Vol. 10 2020 0000-00-00 Zaloznikova NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-097 2045-7022 10.1186/s13601-020-00367-2 43048963 31507929 s13601-020-00367-2.pdf s13601-020-00367-2.pdf 1 1AED6D7955F03FD6D5BAE345391247D4 ea9f4a758b7ae762d051287ca0807449e43369ac50be402913edd572672b24aa 70bc8d09-17b6-11ed-b6b8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=16699 Univerzitetna klinika za pljučne bolezni in alergijo Golnik 0 0 0