| Naslov: | Chemokines during anaphylaxis : the importance of CCL2 and CCL2-dependent chemotactic activity for basophils |
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| Avtorji: | ID Vantur, Romana, Klinika Golnik (Avtor)
ID Rihar, Maruša, Klinika Golnik (Avtor)
ID Koren, Ana, Klinika Golnik (Avtor)
ID Rijavec, Matija, Klinika Golnik, Biotehniška fakulteta UL (Avtor)
ID Kopač, Peter, Klinika Golnik, Medicinska fakulteta UL (Avtor)
ID Bidovec, Urška, Klinika Golnik (Avtor)
ID Eržen, Renato, Klinika Golnik, Medicinska fakulteta UL (Avtor)
ID Korošec, Peter, Klinika Golnik (Avtor) |
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| Datoteke: |  PDF - Predstavitvena datoteka, prenos (2,04 MB)
MD5: 1AED6D7955F03FD6D5BAE345391247D4
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
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| Povzetek: | Background: The role of chemokines in anaphylaxis is unclear. Methods: We prospectively recruited 49 patients presenting to the emergency department with an acute episode of anaphylaxis and 28 healthy subjects. We measured serum levels of the chemokines CCL2, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL21, CCL22, CCL24, and CCL26, tryptase, the absolute number of circulating basophils, monocytes, lymphocytes, and PMNs, and whole blood FCER1A, CPA3 and HDC gene expression at two time points: during the anaphylactic episode and in convalescent samples collected approximately 3 months later. We then investigated the in vitro chemotactic activity of chemokines induced during anaphylaxis for the in vitro migration of the corresponding cells. Results: Only CCL2 chemokine levels were signifcantly increased in anaphylaxis samples (median 514 pg/ml) compared to convalescent samples (284 pg/ml, P<0.0001) and healthy subjects (279 pg/ml, P<0.0001); there was no signifcant diference in any of the other chemokines. There was a signifcant positive correlation between the rates of increase of serum CCL2 (median [range]: 106.0% [-44.7% to 557.4%]) and tryptase (133.8% [-6.6% to 893.4%]; r=0.68, P<0.0001) and between the acute concentration of serum CCL2 and the acute concentration of serum tryptase (r=0.77, P<0.0001). The number of circulating basophils, but not other blood cells, signifcantly decreased during anaphylaxis (median 5.0 vs. 19.1 cells/[micro]l in convalescent samples; P<0.0001); a decrease in whole-blood gene expression of basophil markers (P</=0.0018) confirmed these changes. Anaphylactic serum enhances the in vitro migration of basophils via CCL2-dependent chemotactic activity; in contrast, no CCL2-dependent chemotactic activity was observed for convalescent samples. Conclusions: Our findings imply an important and specifc role for CCL2-mediated chemotactic activity in the pathophysiology of human anaphylaxis. |
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| Ključne besede: | anaphylaxis, chemokines, tryptases, basophils, chemotaxis, CCL2, cell migration |
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| Verzija publikacije: | Objavljena publikacija |
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| Kraj izida: | Velika Britanija |
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| Založnik: | Springer Nature |
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| Leto izida: | 2020 |
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| Št. strani: | str. 1-11 |
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| Številčenje: | Vol. 10 |
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| PID: | 20.500.12556/DiRROS-13682  |
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| UDK: | 616-097 |
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| ISSN pri članku: | 2045-7022 |
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| DOI: | 10.1186/s13601-020-00367-2 |
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| COBISS.SI-ID: | 43048963 |
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| Avtorske pravice: | © The Author(s) 2020 |
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| Opomba: | Nasl. z nasl. zaslona;
Soavtorji: Maruša Rihar, Ana Koren, Matija Rijavec, Peter Kopač, Urška Bidovec-Stojković, Renato Eržen, Peter Korošec;
Opis vira z dne 16. 12. 2020;
Št. članka: 63;
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| Datum objave v DiRROS: | 18.01.2021 |
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| Število ogledov: | 1896 |
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| Število prenosov: | 775 |
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| Metapodatki: |    |
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