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Naslov:Detection of EGFR variants in plasma : a multilaboratory comparison of a real-time PCR EGFR mutation test in Europe
Avtorji:ID Keppens, Cleo (Avtor)
ID Palma, John (Avtor)
ID Das, Partha (Avtor)
ID Scudder, Sidney (Avtor)
ID Wen, Wei (Avtor)
ID Normanno, Nicola (Avtor)
ID van Krieken, Han J. J. M. (Avtor)
ID Sacco, Alessandra (Avtor)
ID Fenizia, Francesca (Avtor)
ID Gonzalez de Castro, David (Avtor)
ID Hönigschnabl, Selma (Avtor)
ID Kern, Izidor, Klinika Golnik (Avtor)
ID Lopez-Rios, Fernando (Avtor)
ID Lozano, Maria D. (Avtor)
ID Marchetti, Antonio (Avtor)
ID Halfon, Philippe (Avtor)
ID Schuuring, Ed (Avtor)
ID Setinek, Ulrike (Avtor)
ID Sorensen, Boe (Avtor)
ID Taniere, Phillipe (Avtor)
ID Tiemann, Markus (Avtor)
ID Vosmikova, Hana (Avtor)
ID Dequeker, Elisabeth (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite https://jmd.amjpathol.org/article/S1525-1578(17)30514-7/fulltext
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
Povzetek:Molecular testing of EGFR is required to predict the response likelihood to targeted therapy in non-small cell lung cancer. Analysis of circulating tumor DNA in plasma may complement limitations of tumor tissue. This study evaluated the interlaboratory performance and reproducibility of a real-time PCR EGFR mutation test (cobas EGFR Mutation Test v2) to detect EGFR variants in plasma. Fourteen laboratories received two identical panels of 27 single-blinded plasma samples. Samples were wild type or spiked with plasmid DNA to contain seven common EGFR variants at six predefined concentrations from 50 to 5000 copies per milliliter. The circulating tumor DNA was extracted by a cell-free circulating DNA sample preparation kit (cobas cfDNA Sample Preparation Kit), followed by duplicate analysis with the real-time PCR EGFR mutation test (Roche Molecular Systems, Pleasanton, CA). Lowest sensitivities were obtained for the c.2156G>C p.(Gly719Ala) and c.2573T>G p.(Leu858Arg) variants for the lowest target copies. For all other variants, sensitivities varied between 96.3% and 100.0%. All specificities were 98.8% to 100.0%. Coefficients of variation indicated good intralaboratory and interlaboratory repeatability and reproducibility but increased for decreasing concentrations. Prediction models revealed a significant correlation for all variants between the predefined copy number and the observed semiquantitative index values, which reflect the samples' plasma mutation load. This study demonstrates an overall robust performance of the real-time PCR EGFR mutation test kit in plasma. Prediction models may be applied to estimate the plasma mutation load for diagnostic or research purposes.
Ključne besede:non-small cell lung cancer, plasma, EGFR, molecular testing
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Kraj izida:ZDA
Založnik:Elsevier
Leto izida:2018
Št. strani:str. 483-494
Številčenje:Vol. 20, no. 4
PID:20.500.12556/DiRROS-12695 Novo okno
UDK:616-006
ISSN pri članku:1943-7811
DOI:10.1016/j.jmoldx.2018.03.006 Novo okno
COBISS.SI-ID:2048314481 Novo okno
Avtorske pravice:2018 American Society for Investigative Pathology and the Association for Molecular Pathology
Opomba:Soavtorji: John F. Palma, Partha M. Das, Sidney Scudder, Wei Wen, Nicola Normanno, J. Han van Krieken, Alessandra Sacco, Francesca Fenizia, David Gonzalez de Castro, Selma Hönigschnabl, Izidor Kern, Fernando Lopez-Rios, Maria D. Lozano, Antonio Marchetti, Philippe Halfon, Ed Schuuring, Ulrike Setinek, Boe Sorensen, Phillipe Taniere, Markus Tiemann, Hana Vosmikova, and Elisabeth M.C. Dequeker; Nasl. z nasl. zaslona; Opis vira z dne 9. 7. 2018;
Datum objave v DiRROS:23.11.2020
Število ogledov:1723
Število prenosov:363
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Gradivo je del revije

Naslov:The journal of molecular diagnostics
Skrajšan naslov:J. mol. diagn.
Založnik:American Society for Investigative Pathology and the Association for Molecular Pathology
ISSN:1943-7811
COBISS.SI-ID:3615508 Novo okno

Sekundarni jezik

Jezik:Ni določen
Ključne besede:nedrobnocelični pljučni rak, plazma, molekularno testiranje


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