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An outbreak of Verticillium dahliae on sycamore maple in a forest stand in Slovenia
Ana Brglez, Zina Devetak, Nikica Ogris, Sebastjan Radišek, Barbara Piškur, 2024, izvirni znanstveni članek

Povzetek: Verticillium dahliae Kleb., the causal agent of Verticillium wilts, is a devastating plant disease affecting many plant species. Fungus V. dahliae was detected in a partially artificially established Acer pseudoplatanus L. forest stand in central Slovenia. This finding incited further study about the risk of different sources of V. dahliae isolates for maples in forests and the pathogenicity of three V. dahliae isolates of different origins was tested on saplings of A. pseudoplatanus, A. platanoides L., and A. campestre L. The inoculated saplings exhibited disease symptoms, i.e., leaf necrosis and wilting. At the end of the pathogenicity test, typical xylem browning was visible on the cross-sections, and the pathogen was successfully re-isolated. The isolates showed significant differences in their pathogenicity to specific maple hosts, with the agricultural isolate (originated from bell pepper) being the most aggressive on all three maple species. The disease severity index (DSI) and relative area under the disease progress curve (rAUDPC), as well as the success of re-isolation, indicate that A. platanoides is the most susceptible to inoculation with V. dahliae. In addition, significant differences in sapling biomass were observed between treated and control plants. These results suggest that maples in forest stands are threatened by V. dahliae, and biosecurity measures should be considered and implemented in forest management to reduce the transmission and potential spread of the pathogen.
Ključne besede: Verticillium wilt, Acer spp., pathogenicity test, Disease severity index, DSI, Area under the disease progress curve, AUDPC, biosecurity
Objavljeno v DiRROS: 07.02.2024; Ogledov: 104; Prenosov: 47
.pdf Celotno besedilo (1,75 MB)
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The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.)
Zina Devetak, Andreja Kavčič, Maarten De Groot, Barbara Piškur, 2023, izvirni znanstveni članek

Povzetek: One of the key steps of the molecular identification of bark beetles is obtaining a sufficient quantity of high-quality DNA extract. In this study, we investigated the influence of different storage procedures for Ips typographus (L.) specimens and various DNA extraction protocols on the quantity and quality of DNA intended for use in molecular diagnostics. Adult beetles were frozen at -20 °C, either dry or in ethanol. We tested four different protocols for DNA extraction. We compared the quantity of extracted DNA and assessed its quality with PCR and Sanger sequencing. Different storage protocols had no significant effect on the quantity of DNA extracted. However, freezing specimens in ethanol provided higher-quality DNA for molecular applications. Only two of the extraction protocols produced sequenceable amplicons, and the difference in the amount of extracted DNA between them was not significant. We propose the optimal combination of storing specimens in ethanol at -20°C and using the Nucleospin Insect DNA extraction kit from Macherey Nagel, enabling a timeefficient identification process.
Ključne besede: early detection, specimen storage, total DNA extraction, PCR, polymerase chain reaction, Sanger sequencing, molecular diagnostics
Objavljeno v DiRROS: 02.02.2024; Ogledov: 244; Prenosov: 75
.pdf Celotno besedilo (1,17 MB)
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Patogenost izolatov izbranih vrst gliv na Pinus sylvestris L. IN P. nigra Arnold
Ana Brglez, Barbara Piškur, Nikica Ogris, 2023, izvirni znanstveni članek

Povzetek: Na sadikah rdečega in črnega bora smo testirali patogenost osmih izbranih izolatov gliv (Corinectria fuckeliana, Diaporthe eres, Diplodia pinea, Fusarium sp. 1 in 2, Fusicolla sp., Nectria dematiosa in Tympanis sp.), ki smo jih izolirali iz obolelih borovih dreves v Sloveniji v letih 2020 in 2021. Inokulirali smo skupno 115 sadik borov (vključno s kontrolo), periodično smo preverjali njihovo zdravstveno stanje in ob prvih znakih sušenja opravili reizolacije iz robov nastalih nekroz. Reizolacije so bile uspešne le v primeru inokulacije z vrstami gliv Diplodia pinea, Diaporthe eres in Fusarium sp. 2. Povprečna dolžina nekroz sadik rdečega bora, inokuliranih z D. pinea, Di. eres in Tympanis sp., se je statistično značilno razlikovala od kontrolnih sadik (p < 0,05). Pri sadikah črnega bora pa so se za statistično značilne izkazale povprečne dolžine nekroz pri kontroli in inokulacijah z glivama D. pinea in Tympanis sp. (p < 0,05).
Ključne besede: test patogenosti, reizolacije, Pinus sylvestris, rdeči bor, Pinus nigra, črni bor
Objavljeno v DiRROS: 25.01.2024; Ogledov: 257; Prenosov: 101
.pdf Celotno besedilo (2,17 MB)
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