261. Platformne storitveAna Robnik, Andrej Ciglič, Aleš Balanč, Dušan Gabrijelčič, Jan Porekar, Arso Savanović, Polona Škraba, Roman Kužnar, Ignac Zupan, 2015, objavljeni znanstveni prispevek na konferenci Objavljeno v DiRROS: 08.12.2020; Ogledov: 1321; Prenosov: 393
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262. Težke odločitve pri zdravljenju bolnikov z vensko trombembolijo in rakomAna Kovač Bograf, Monika Štalc, Gregor Tratar, Alenka Mavri, 2016, pregledni znanstveni članek Povzetek: Venska trombembolija (VTE) je pogost zaplet pri bolnikih z rakom. Zdravljenje VTE pri teh bolnikih predstavlja velik izziv, saj je pri bolnikih z rakom dodatno povečano tako tveganje za trombembolične zaplete kot tudi tveganje za krvavitve. Predstavili bomo nekaj kliničnih situacij, s katerimi se v klinični praksi pogosto srečamo in predstavljajo dileme pri antikoagulacijskem zdravljenju bolnikov z VTE ter rakom, in sicer ponovitve VTE kljub zdravljenju, pojav trombocitopenije, tumorje osrednjega živčevja, uporabo filtrov v spodnji votli veni, uporabo novih peroralnih antikoagulacijskih zdravil in pojav naključno odkrite VTE.
Ključne besede: venska trombembolija, rak (medicina), zdravljenje
Objavljeno v DiRROS: 03.12.2020; Ogledov: 1368; Prenosov: 580
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264. Quantitative, absolute count-based T cell analysis of CD69 upregulation as a new methodology for in vitro diagnosis of delayed-type nickel hypersensitivityAna Koren, Mira Šilar, Helena Rupnik, Mihaela Zidarn, Peter Korošec, 2019, izvirni znanstveni članek Povzetek: Background: T cells play a major role in delayed-type hypersensitivity reactions. Their reactivity can be assessed by measuring the upregulation of the activation marker CD69, followed by proliferation and cytokine production. The aim of our study was to develop a novel, whole blood-based, quantitative, absolute count activation index (AI) analysis of CD69 upregulation on different subsets of T cells in nickel hypersensitive patients and compare it with the previously reported approaches. Methods: Ten patients with nickel allergy and nine healthy controls were included. CD69 expression of CD3+, CD3+CD4+ and CD3+CD8+ T cells in heparinized blood was determined with flow cytometry after incubation with nickel sulfate for 48 h. The absolute cell count of CD69+ cells was determined with microbeads. The production of the cytokines IL-2, IL-5, IL-13, and IFN-[gamma] was determined after nickel sulfate stimulation of PBMNCs for 48 h. Results: We showed that the most sensitive methodology is the absolute AI, which was calculated as the ratio between the absolute count of CD69-positive T cells stimulated with nickel and the absolute count of CD69-positive T cells in non-stimulated blood. This novel quantitative approach was more discriminative than the previously reported approaches in which T cell CD69 percentage AI and cytokine production are measured. Conclusions: Our results demonstrated that measuring the absolute CD69 AI is an accurate new approach to quantify antigen-specific T cells in the blood of patients with hypersensitivity reactions to nickel. This approach may be useful for better in vitro assessment of patients with delayed-type hypersensitivity reactions.
Ključne besede: allergy and immunology, delayed hypresensitivity, nickel, blood, CD antigenes, CD69
Objavljeno v DiRROS: 18.11.2020; Ogledov: 1228; Prenosov: 316
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265. Using citizen science to monitor the spread of tree pests and diseases : outcomes of two projects in Slovenia and the UKPeter Crow, Ana Pérez-Sierra, Andreja Kavčič, Kate Lewthwaite, Marija Kolšek, Nikica Ogris, Barbara Piškur, Jana Kus Veenvliet, Simon Zidar, Suzanne Sancisi-Frey, Maarten De Groot, 2020, izvirni znanstveni članek Povzetek: The trees and forests of Europe are increasingly under threat from new pests and diseases that have originated in other parts of the world. Early detection of alien species when they first appear in European countries allows rapid response and offers the best chance to mitigate against their establishment and spread. Citizen science initiatives such as LIFE ARTEMIS in Slovenia, and Observatree in the UK, provide members of the public with the necessary training and educational resource to identify these tree pests and diseases and report them to the appropriate authorities, thereby increasing the level of surveillance and the capacity of the early warning system. This paper summarises some of the outcomes of these two projects and how they have both become integral parts of the official forest and tree health monitoring systems within their respective countries of Slovenia and the United Kingdom.
Ključne besede: invasive alien species, plant health, tree health, forest health, early warning, rapid response
Objavljeno v DiRROS: 16.11.2020; Ogledov: 1734; Prenosov: 859
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267. Pomen biovarnosti za zdravje gozdov : pregled izkušenj iz tujine in predlogi za SlovenijoAna Brglez, Peter Smolnikar, Barbara Piškur, 2020, strokovni članek Povzetek: V letu 2020 obeležujemo mednarodno leto zdravja rastlin. V kmetijstvu in vrtnarstvu je pojem zdravja rastlin dobro znan, medtem ko se za naravno okolje vse premalokrat omenja. Gozdove ogrožajo številni domači in tujerodni organizmi, ki jim naša dejavnost v okolju omogoča vse lažje in hitrejše širjenje v prostoru. Za preprečitev oz. omejevanje širjenja je ključno delovanje fitosanitarnih inšpekcij in nadzora na mejah ob vstopu tujega blaga, na lokalnem nivoju pa ozaveščenost strokovnih služb, lastnikov zemljišč ter vseh obiskovalcev narave, da upoštevajo osnovne ti. biovarnostne ukrepe. Biovarnost je skupek ukrepov, ki zmanjšajo ali v celoti preprečijo vnos in prenos škodljivih organizmov z ene lokacije na drugo. Po navadi ukrepi zajemajo čiščenje oblačil, obutve, vozil in opreme vseh organskih ostankov rastlin in zemlje ter pregled notranjosti vozila glede prisotnosti žuželk. V prispevku predstavljamo kot primer dobre prakse ozaveščanja o pomenu biovarnosti v gozdovih obširno akcijo Združenega kraljestva Keep it clean ter iščemo ovire in priložnosti za rabo pri nas. V Sloveniji je pojem biovarnosti uveljavljen v kmetijstvu (npr. v prašičjereji in hmeljarstvu), v gozdarstvu pa je dokaj neznan. Z zakonsko dovoljenim prostim dostopom v gozd, gosto mrežo gozdnih cest in v zadnjem času povečanim obiskom gozdov je ob morebitni prisotnosti škodljivih organizmov potencial za njihovo hitro razširjenje izjemno velik. Vendar pa je tudi prostora za izboljšanje sedanje situacije še veliko. Med priložnostmi lahko izpostavimo ozaveščanje v smeri higiene in ukrepov, ki so potrebni za preprečitev vnosa in prenosa škodljivih organizmov, posodobitev veljavne zakonodaje na področju zdravja rastlin in gozdarstva, njuno uskladitev z veljavno evropsko zakonodajo ter nadzor nad njihovim izvajanjem. Nikakor pa ne smemo pozabiti na največjo priložnost vsakega izmed nas, da se vede odgovorno do okolja, v katerem živimo.
Ključne besede: biovarnost, biovarnostni ukrepi, zdravje gozdov, varstvo gozdov, škodljivi organizmi
Objavljeno v DiRROS: 14.11.2020; Ogledov: 1829; Prenosov: 485
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269. CD3+CD4-CD8- mucosal T cells are associated with uncontrolled chronic rhinosinusitis with nasal polypsTanja Soklič, Mira Šilar, Matija Rijavec, Ana Koren, Izidor Kern, Irena Hočevar-Boltežar, Peter Korošec, 2019, kratki znanstveni prispevek Povzetek: Increased mucosal double-negative (DN) CD3+CD4-CD8- T cells were found for the first time in CRS and were much more abundant in uncontrolled CRSwNP than in well-controlled CRSwNP.
Ključne besede: chronic rhinosinusitis, CD3+ T-cells, CD4- T-cells, CD8- T-cells
Objavljeno v DiRROS: 22.10.2020; Ogledov: 1595; Prenosov: 516
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270. In vitro interactions between Eutypella parasitica and some frequently isolated fungi from the wood of the dead branches of young Sycamore Maple (Acer pseudoplatanus)Ana Brglez, Barbara Piškur, Nikica Ogris, 2020, izvirni znanstveni članek Povzetek: The ten most frequently isolated fungi from the wood of the dead branches of Acer pseudoplatanus L. were tested in dual cultures to evaluate their in vitro antagonistic activity against Eutypella parasitica R.W. Davidson and R.C. Lorenz, the causative agent of a destructive disease of maples in Europe and North America. The tested fungi, treated also as challenge isolates, were Diaporthe sp., Eutypa sp., Eu. maura, E. parasitica, Fusarium avenaceum, Neocucurbitaria acerina, Neonectria sp., Peniophora incarnata, Petrakia irregularis, and Phomopsis pustulata. The antagonistic ability of each challenge isolate was evaluated by calculating an index of antagonism (AI) based on the interaction type in the dual cultures. The results of competition between the fungal isolates were quantified after re-isolations from the interaction zone (s). The dual cultures revealed two main types of competitive interactions: Deadlock, consisting of mutual inhibition after mycelial contact or at a distance, and replacement, reflecting in the inhibition of E. parasitica, followed by partial overgrowth by the replacing fungus. Statistical analysis showed significant differences in average AI and s of challenge isolates between different dual culture assays. Based on the results of the antagonism index, Eutypa sp., Eu. maura, Neonectria sp., and P. incarnata had the highest inhibitory effect on E. parasitica growth and were recognized as the most promising candidates for further biocontrol studies of E. parasitica. The mycelium of E. parasitica at the interaction zones remained mostly viable, except in dual cultures with Eutypa sp., F. avenaceum, and Neonectria sp., where re-isolations did not yield any colony of the E. parasitica isolate. Based on the results, we assume that E. parasitica is a weak competitor, which invests less energy in direct mycelial competition. We discuss the potential of the observed antagonists as a possible biocontrol of Eutypella canker of maple. Nevertheless, additional experiments should be performed for a solid conclusion about competitive ability of E. parasitica and usefulness of antagonists as biocontrol.
Ključne besede: Eutypella parasitica, dual culture, hyphal interaction, deadlock, replacement, competition, antagonism, inhibition, re-isolation, biocontrol
Objavljeno v DiRROS: 19.10.2020; Ogledov: 1332; Prenosov: 872
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