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Histology basics and cell death detection in honeybee tissue
Maja Ivana Smodiš Škerl, izvirni znanstveni članek

Povzetek: Honeybees (Apis mellifera L.) inside the hive (nurse workers and other hive bees) and outside the hive (foragers) are exposed to climate and weather changes, various pesticides, pathogens, and malnutrition, mainly entering through the mouth and primarily affecting the digestive tracts of adult bees. To understand and prevent the effects of such external and internal stressors on honeybees, one useful research method is the immunohistochemical method. A basic protocol is described to prepare the midgut (ventriculus) and hypopharyngeal glands (HPGs) of adult bees for histological analysis. A detailed methodology is described to assess the level of cell damage and distinguish necrosis from programmed cell death (apoptosis) as a natural process of tissue regeneration. The results of adult honeybee treatment with oxalic acid and pesticides (insecticide and acaricide) and the determination of cell death in the ventriculus and HPGs are presented. The pros and cons of the methodology are also discussed.
Ključne besede: cell death, honeybee, histology, environment
Objavljeno v DiRROS: 08.07.2022; Ogledov: 92; Prenosov: 16
.pdf Celotno besedilo (1,31 MB)
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Hyperspectral remote sensing of grapevine drought stress
Monika Zovko, Uroš Žibrat, Matej Knapič, Marina Bubalo, Davor Romić, 2019, izvirni znanstveni članek

Ključne besede: vineyard, soil, precision, irrigation, water stress, hyperspectral imagery
Objavljeno v DiRROS: 14.05.2020; Ogledov: 1726; Prenosov: 791
.pdf Celotno besedilo (1,70 MB)

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