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Iskalni niz: "vrsta gradiva" (1) AND "polno besedilo" AND "organizacija" (Kemijski inštitut) .

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A small bacteriophage protein determines the hierarchy over co-residential jumbo phage in Bacillus thuringiensis serovar israelensis
Anja Pavlin, Anže Lovše, Gregor Bajc, Jan Otoničar, Amela Kujović, Živa Lengar, Ion Gutiérrez-Aguirre, Rok Kostanjšek, Janez Konc, Nadine Fornelos, Matej Butala, 2022, izvirni znanstveni članek

Povzetek: Bacillus thuringiensis serovar israelensis is the most widely used biopesticide against insects, including vectors of animal and human diseases. Among several extrachromosomal elements, this endospore-forming entomopathogen harbors two bacteriophages: a linear DNA replicon named GIL01 that does not integrate into the chromosome during lysogeny and a circular-jumbo prophage known as pBtic235. Here, we show that GIL01 hinders the induction of cohabiting prophage pBtic235. The GIL01-encoded small protein, gp7, which interacts with the host LexA repressor, is a global transcription regulator and represses the induction of pBtic235 after DNA damage to presumably allow GIL01 to multiply first. In a complex with host LexA in stressed cells, gp7 down-regulates the expression of more than 250 host and pBtic235 genes, many of which are involved in the cellular functions of genome maintenance, cell-wall transport, and membrane and protein stability. We show that gp7 homologs that are found exclusively in bacteriophages act in a similar fashion to enhance LexA’s binding to DNA, while likely also affecting host gene expression. Our results provide evidence that GIL01 influences both its host and its co-resident bacteriophage.
Objavljeno v DiRROS: 18.07.2024; Ogledov: 15; Prenosov: 8
.pdf Celotno besedilo (3,46 MB)
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Cytotoxicity and antibacterial efficacy of betaine- and choline-substituted polymers
Lucija Jurko, Damjan Makuc, Alja Štern, Janez Plavec, Bojana Žegura, Perica Bošković, Rupert Kargl, 2023, izvirni znanstveni članek

Povzetek: Cationic charge has been widely used to increase polymer adsorption and flocculation of dispersions or to provide antimicrobial activity. In this work, cationization of hydroxyethyl cellulose (HEC) and polyvinyl alcohol (PVA) was achieved by covalently coupling betaine hydrochloride and choline chloride to the polymer backbones through carbonyl diimidazole (CDI) activation. Two approaches for activation were investigated. CDI in excess was used to activate the polymers’ hydroxyls followed by carbonate formation with choline chloride, or CDI was used to activate betaine hydrochloride, followed by ester formation with the polymers’ hydroxyls. The first approach led to a more significant cross-linking of PVA, but not of HEC, and the second approach successfully formed ester bonds. Cationic, nitrogen-bearing materials with varying degrees of substitution were obtained in moderate to high yields. These materials were analyzed by Fourier transform infrared spectroscopy, nuclear magnetic resonance, polyelectrolyte titration, and kaolin flocculation. Their dose-dependent effect on the growth of Staphylococcus aureus and Pseudomonas aeruginosa, and L929 mouse fibroblasts, was investigated. Significant differences were found between the choline- and betaine-containing polymers, and especially, the choline carbonate esters of HEC strongly inhibited the growth of S. aureus in vitro but were also cytotoxic to fibroblasts. Fibroblast cytotoxicity was also observed for betaine esters of PVA but not for those of HEC. The materials could potentially be used as antimicrobial agents for instance by coating surfaces, but more investigations into the interaction between cells and polysaccharides are necessary to clarify why and how bacterial and human cells are inhibited or killed by these derivatives, especially those containing choline.
Ključne besede: hydroxyethyl cellulose, polyvinyl alcohol, antimicrobial, S. aureus, P. aeruginosa, L929 mouse fibroblast, cationic polymer
Objavljeno v DiRROS: 12.07.2024; Ogledov: 70; Prenosov: 49
.pdf Celotno besedilo (3,21 MB)
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Composition of colloidal organic matter in phytoplankton exudates
Katja Klun, Primož Šket, Alfred Beran, Ingrid Falnoga, Jadran Faganeli, 2023, izvirni znanstveni članek

Objavljeno v DiRROS: 12.07.2024; Ogledov: 71; Prenosov: 27
.pdf Celotno besedilo (1,31 MB)
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Optimization of starch–tannin adhesives for solid wood gluing
Annalisa Magnabosco, Illya Kulyk, Maurizio Avancini, Primož Šket, Jonas Eckardt, Emanuele Cesprini, Francesco Marinello, Gianluca Tondi, 2024, izvirni znanstveni članek

Objavljeno v DiRROS: 01.07.2024; Ogledov: 114; Prenosov: 56
.pdf Celotno besedilo (2,93 MB)
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