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Iskalni niz: "polno besedilo" AND "organizacija" (Kemijski inštitut) .

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Hyaluronic acid conjugates of glycine peptides and L-tryptophan
Fazilet Gürer, Tamilselvan Mohan, Matej Bračič, Ariana Barlič, Damjan Makuc, Janez Plavec, Karin Stana-Kleinschek, Rupert Kargl, 2024, izvirni znanstveni članek

Objavljeno v DiRROS: 24.07.2024; Ogledov: 7; Prenosov: 4
.pdf Celotno besedilo (5,18 MB)
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Solution-state structure of a long-loop G-quadruplex formed within promoters of plasmodium falciparum B var genes
Marina Juribašić Kulcsár, Valerie Gabelica, Janez Plavec, 2024, izvirni znanstveni članek

Objavljeno v DiRROS: 24.07.2024; Ogledov: 7; Prenosov: 3
.pdf Celotno besedilo (1,87 MB)
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Structural basis for the multitasking nature of the potato virus Y coat protein
Andreja Kežar, Luka Kavčič, Martin Pólak, Jiři Nováček, Ion Gutiérrez-Aguirre, Magda Tušek-Žnidarič, Anna Coll Rius, Katja Stare, Kristina Gruden, Maja Ravnikar, David Pahovnik, Ema Žagar, Franci Merzel, Gregor Anderluh, Marjetka Podobnik, 2019, izvirni znanstveni članek

Povzetek: Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY’s flexuous virions, revealing a previously unknown lumenal interplay between extended carboxyl-terminal regions of the coat protein units and viral RNA. RNA–coat protein interactions are crucial for the helical configuration and stability of the virion, as revealed by the unique near-atomic structure of RNA-free virus-like particles. The structures offer the first evidence for plasticity of the coat protein’s amino- and carboxyl-terminal regions. Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. Moreover, the high modularity of PVY virus-like particles suggests their potential as a new molecular scaffold for nanobiotechnological applications.
Ključne besede: plant pathogens, potato virus Y, viral RNA
Objavljeno v DiRROS: 23.07.2024; Ogledov: 30; Prenosov: 5
.pdf Celotno besedilo (4,43 MB)
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α-hydrazino acid insertion governs peptide organization in solution by local structure ordering
Luka Kavčič, Gregor Ilc, Baifan Wang, Kristina Vlahoviček-Kahlina, Ivanka Jerić, Janez Plavec, 2024, izvirni znanstveni članek

Objavljeno v DiRROS: 23.07.2024; Ogledov: 22; Prenosov: 10
.pdf Celotno besedilo (3,99 MB)
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A small bacteriophage protein determines the hierarchy over co-residential jumbo phage in Bacillus thuringiensis serovar israelensis
Anja Pavlin, Anže Lovše, Gregor Bajc, Jan Otoničar, Amela Kujović, Živa Lengar, Ion Gutiérrez-Aguirre, Rok Kostanjšek, Janez Konc, Nadine Fornelos, Matej Butala, 2022, izvirni znanstveni članek

Povzetek: Bacillus thuringiensis serovar israelensis is the most widely used biopesticide against insects, including vectors of animal and human diseases. Among several extrachromosomal elements, this endospore-forming entomopathogen harbors two bacteriophages: a linear DNA replicon named GIL01 that does not integrate into the chromosome during lysogeny and a circular-jumbo prophage known as pBtic235. Here, we show that GIL01 hinders the induction of cohabiting prophage pBtic235. The GIL01-encoded small protein, gp7, which interacts with the host LexA repressor, is a global transcription regulator and represses the induction of pBtic235 after DNA damage to presumably allow GIL01 to multiply first. In a complex with host LexA in stressed cells, gp7 down-regulates the expression of more than 250 host and pBtic235 genes, many of which are involved in the cellular functions of genome maintenance, cell-wall transport, and membrane and protein stability. We show that gp7 homologs that are found exclusively in bacteriophages act in a similar fashion to enhance LexA’s binding to DNA, while likely also affecting host gene expression. Our results provide evidence that GIL01 influences both its host and its co-resident bacteriophage.
Objavljeno v DiRROS: 18.07.2024; Ogledov: 66; Prenosov: 28
.pdf Celotno besedilo (3,46 MB)
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Cytotoxicity and antibacterial efficacy of betaine- and choline-substituted polymers
Lucija Jurko, Damjan Makuc, Alja Štern, Janez Plavec, Bojana Žegura, Perica Bošković, Rupert Kargl, 2023, izvirni znanstveni članek

Povzetek: Cationic charge has been widely used to increase polymer adsorption and flocculation of dispersions or to provide antimicrobial activity. In this work, cationization of hydroxyethyl cellulose (HEC) and polyvinyl alcohol (PVA) was achieved by covalently coupling betaine hydrochloride and choline chloride to the polymer backbones through carbonyl diimidazole (CDI) activation. Two approaches for activation were investigated. CDI in excess was used to activate the polymers’ hydroxyls followed by carbonate formation with choline chloride, or CDI was used to activate betaine hydrochloride, followed by ester formation with the polymers’ hydroxyls. The first approach led to a more significant cross-linking of PVA, but not of HEC, and the second approach successfully formed ester bonds. Cationic, nitrogen-bearing materials with varying degrees of substitution were obtained in moderate to high yields. These materials were analyzed by Fourier transform infrared spectroscopy, nuclear magnetic resonance, polyelectrolyte titration, and kaolin flocculation. Their dose-dependent effect on the growth of Staphylococcus aureus and Pseudomonas aeruginosa, and L929 mouse fibroblasts, was investigated. Significant differences were found between the choline- and betaine-containing polymers, and especially, the choline carbonate esters of HEC strongly inhibited the growth of S. aureus in vitro but were also cytotoxic to fibroblasts. Fibroblast cytotoxicity was also observed for betaine esters of PVA but not for those of HEC. The materials could potentially be used as antimicrobial agents for instance by coating surfaces, but more investigations into the interaction between cells and polysaccharides are necessary to clarify why and how bacterial and human cells are inhibited or killed by these derivatives, especially those containing choline.
Ključne besede: hydroxyethyl cellulose, polyvinyl alcohol, antimicrobial, S. aureus, P. aeruginosa, L929 mouse fibroblast, cationic polymer
Objavljeno v DiRROS: 12.07.2024; Ogledov: 75; Prenosov: 54
.pdf Celotno besedilo (3,21 MB)
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Composition of colloidal organic matter in phytoplankton exudates
Katja Klun, Primož Šket, Alfred Beran, Ingrid Falnoga, Jadran Faganeli, 2023, izvirni znanstveni članek

Objavljeno v DiRROS: 12.07.2024; Ogledov: 81; Prenosov: 38
.pdf Celotno besedilo (1,31 MB)
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