1. Cryo-EM structures of a protein pore reveal a cluster of cholesterol molecules and diverse roles of membrane lipidsGašper Šolinc, Marija Srnko, Franci Merzel, Ana Crnković, Mirijam Kozorog, Marjetka Podobnik, Gregor Anderluh, 2025, izvirni znanstveni članek Povzetek: The structure and function of membrane proteins depend on their interactions with lipids that constitute membranes. Actinoporins are α-pore-forming proteins that bind preferentially to sphingomyelin-containing membranes, where they oligomerize and form transmembrane pores. Through a comprehensive cryo-electron microscopic analysis of a pore formed by an actinoporin Fav from the coral Orbicella faveolata, we show that the octameric pore interacts with 112 lipids in the upper leaflet of the membrane, reveal the roles of lipids, and demonstrate that the actinoporin surface is suited for binding multiple receptor sphingomyelin molecules. When cholesterol is present in the membrane, it forms a cluster of four molecules associated with each protomer. Atomistic simulations support the structural data and reveal additional effects of the pore on the lipid membrane. These data reveal a complex network of protein-lipid and lipid-lipid interactions and an underrated role of lipids in the structure and function of transmembrane protein complexes.
Objavljeno v DiRROS: 12.05.2025; Ogledov: 66; Prenosov: 33
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6. Microfluidics for electrochemical energy conversion and storage : prospects toward sustainable ammonia productionErvin Rems, Ana Herceg, Desislava Apostolova, Robert Dominko, Primož Jovanovič, Boštjan Genorio, 2025, pregledni znanstveni članek Ključne besede: ammonia, electrochemistry, energy conversion, microreactors, nitrogen reduction reaction
Objavljeno v DiRROS: 16.04.2025; Ogledov: 166; Prenosov: 66
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7. Osteosarcoma cells and undifferentiated human mesenchymal stromal cells are more susceptible to ferroptosis than differentiated human mesenchymal stromal cellsYuliya D. Smirnova, Dominik Hanetseder, Lukas Derigo, Andreas Sebastian Gasser, Annette Vaglio-Garro, Simon Sperger, Regina Brunauer, Olga S. Korneeva, Johanna Catharina Duvigneau, Darja Marolt Presen, Andrey V. Kozlov, 2025, izvirni znanstveni članek Objavljeno v DiRROS: 15.04.2025; Ogledov: 142; Prenosov: 63
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8. Catalytic hydrodenitrogenation, hydrodeoxygenation and hydrogenation reactions of amides, amines, and nitriles over ▫$NiMoS_X/Al_2O_3$▫ catalysts : mechanisms, kinetics and transportMatej Žula, Vid Bačar, Michal Mazur, Blaž Likozar, 2025, izvirni znanstveni članek Objavljeno v DiRROS: 15.04.2025; Ogledov: 173; Prenosov: 75
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9. Spectroscopic characterization using ▫$^1H$▫ and ▫$^{13}C$▫ nuclear magnetic resonance and computational analysis of the complex of donepezil with 2,6-methyl-β-cyclodextrin and hydroxy propyl methyl celluloseNikoletta Zoupanou, Paraskevi Papakyriakopoulou, Nikitas Georgiou, Antigoni Cheilari, Uroš Javornik, Peter Podbevšek, Demeter Tzeli, Georgia Valsami, Thomas Michael Mavromoustakos, 2025, izvirni znanstveni članek Objavljeno v DiRROS: 15.04.2025; Ogledov: 120; Prenosov: 63
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10. Efficient and selective biosynthesis of a precursor-directed FK506 analogue : paving the way for click chemistryDušan Goranovič, Branko Jenko, Barbara Ramšak, Ajda Podgoršek Berke, Leon Bedrač, Jaka Horvat, Martin Šala, Damjan Makuc, Guilhermina M. Carriche, Luana Silva, Aleksandra Lopez Krol, Alen Pšeničnik, Maria Beatriz Duran Alonso, Martina Avbelj, Stojan Stavber, Janez Plavec, Tim Sparwasser, Rolf Müller, Gregor Kosec, Štefan Fujs, Hrvoje Petković, 2025, izvirni znanstveni članek Povzetek: The medically important immunosuppressant FK506 is a structurally complex macrolactone biosynthesized by a combined polyketide synthase and a nonribosomal peptide synthetase enzyme complex. Its acyltransferase domain 4 (AT4) selects an unusual extender unit, resulting in an allyl moiety on carbon 21 of the macrolactone backbone. Based on the AT4 domain, chemobiosynthetic processes have been developed that enable the introduction of diverse moieties at the carbon 21 position. However, the novel moieties that were introduced into the polyketide backbone are chemically inert. Reported here is a novel and efficient chemobiosynthetic approach that ensures high titer of an FK506 analogue containing a propargyl moiety. The novel FK506 analogue displays lower immunosuppression activity than FK506 with significantly reduced cytotoxicity. More importantly, the propargyl moiety contains a terminal alkyl group, which makes click chemistry reactions possible; this approach may potentially be translated to other medically important drugs of polyketide origin.
Objavljeno v DiRROS: 15.04.2025; Ogledov: 158; Prenosov: 44
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