1. Metabolic rates of groundwater species as a function of body mass and temperatureTiziana Di Lorenzo, Nataša Mori, Tatjana Simčič, 2024, pregledni znanstveni članek Povzetek: Research on the metabolic physiology of groundwater species, particularly regarding oxygen consumption
rates (OCR), has made significant advancement, revealing valuable insights into the adaptations of exclusively
groundwater-dwelling (stygobitic) species. However, a comprehensive understanding of how these metabolic
rates scale with body mass and respond to temperature changes remains elusive. This study aims to bridge this
gap by reviewing published data on OCR across a variety of groundwater organisms to elucidate patterns of
metabolic rates in relation to body size and temperature. We employed a combination of literature review and
quantitative analyses, focusing on the allometric scaling of OCR with body weight and the effect of temperature on metabolic rates. Our findings indicate that OCR scales with body weight in an allometric pattern,
with an inter-species slope of 0.80, suggesting non-isometric scaling. Furthermore, our analysis showed that
stygobitic species’ metabolic rates are less responsive to warming than those of non-stygobitic species at low to
moderate temperatures. However, at higher temperatures, metabolic rates in stygobitic species decline faster
than in non-stygobitic taxa, highlighting a potential vulnerability to global climate change. This study contributes to our understanding of the metabolic strategies of groundwater species, underscoring the need for further
research to fully grasp the eco-evolutionary implications of these findings for groundwater conservation. Ključne besede: allometry, Arrhenius equation, ectotherms, metabolism, oxygen consumption rate, respiration, stygobiont Objavljeno v DiRROS: 13.09.2024; Ogledov: 88; Prenosov: 72 Celotno besedilo (1,63 MB) Gradivo ima več datotek! Več... |
2. Intertwined roles of reactive oxygen species and salicylic acid signaling are crucial for the plant response to biotic stressTjaša Lukan, Anna Coll Rius, 2022, pregledni znanstveni članek Povzetek: One of the earliest hallmarks of plant immune response is production of reactive oxygen species (ROS) in different subcellular compartments, which regulate plant immunity. A suitable equilibrium, which is crucial to prevent ROS overaccumulation leading to oxidative stress, is maintained by salicylic acid (SA), a chief regulator of ROS. However, ROS not only act downstream of SA signaling, but are also proposed to be a central component of a self-amplifying loop that regulates SA signaling as well as the interaction balance between different phytohormones. The exact role of this crosstalk, the position where SA interferes with ROS signaling and ROS interferes with SA signaling and the outcome of this regulation, depend on the origin of ROS but also on the pathosystem. The precise spatiotemporal regulation of organelle-specific ROS and SA levels determine the effectiveness of pathogen arrest and is therefore crucial for a successful immune response. However, the regulatory interplay behind still remains poorly understood, as up until now, the role of organelle-specific ROS and SA in hypersensitive response (HR)-conferred resistance has mostly been studied by altering the level of a single component. In order to address these aspects, a sophisticated combination of research methods for monitoring the spatiotemporal dynamics of key players and transcriptional activity in plants is needed and will most probably consist of biosensors and precision transcriptomics. Ključne besede: plant immune response, reactive oxygen species, ROS, salicylic acid, programmed cell death, biotic stress, biotechnology Objavljeno v DiRROS: 05.08.2024; Ogledov: 179; Prenosov: 91 Celotno besedilo (812,79 KB) Gradivo ima več datotek! Več... |
3. Setting sail for Paris 2024 : retrospective analysis ofworld-class ILCA 7 olympic sailors’ cardiorespiratory fitness (2015–2020)Damir Zubac, Zoran Valić, Vladimir Ivančev, 2024, drugi znanstveni članki Povzetek: The aim of this retrospective analysis was to provide a more comprehensive understanding of the cardiorespiratory profile of world-class ILCA-7 sailors (n = 3, all males), through a longitudinal evaluation offering real-world data on physiological profile and exercise intensity domains. The cardiopulmonary exercise testing (CPET) was performed by the same researchers using the same equipment during the study. Assessments took place twice a year, aligning with major international competition preparations. Participants trained and competed at the same sailing club in Split, Croatia, under consistent supervision from the same team throughout the study, winning a total of 21 medals at major international competitions. The recorded
ranged from 51.7 ± 1.6 to 61.9 ± 3.0 mL min−1 kg−1, respectively. Similarly, peak power output varied from 352 ± 10 to 426 ± 34 W. The changes in physiological responses at the ventilatory thresholds were proportional to the changes in peak cardiorespiratory fitness capacity. Interestingly, the oxygen pulse measured in 2015 was 25 ± 1 mL O2 beat−1. Over the subsequent 6 years, the O2 pulse marginally increased and appeared to stabilize at 27 ± 1 mL O2 beat−1 in 2020, when these athletes were 32 ± 3 years old. This work offers a broader understanding of world-class Olympic sailors’ cardiorespiratory fitness, going beyond the standard assessment of peak
to incorporate an analysis of ventilatory thresholds. While a direct link between cardiorespiratory fitness and competitive success remains ambiguous, the importance of a well-rounded aerobic capacity for excellence in ILCA-7 sailing class is evident. Ključne besede: olypmipcs, oxygen uptake, sailing Objavljeno v DiRROS: 31.07.2024; Ogledov: 229; Prenosov: 258 Celotno besedilo (569,94 KB) Gradivo ima več datotek! Več... |
4. The effects of normobaric and hyperbaric oxygenation on MRI signal intensities in T1-weighted, T2-weighted and FLAIR images in human brainVida Velej, Ksenija Cankar, Jernej Vidmar, 2023, izvirni znanstveni članek Povzetek: Dissolved oxygen has known paramagnetic effects in magnetic resonance imaging (MRI). The aim ofthis study was to compare the effects of normobaric oxygenation (NBO) and hyperbaric oxygenation (HBO) on hu-man brain MRI signal intensities.Patients and methods. Baseline brain MRI was performed in 17 healthy subjects (mean age 27.8 ± 3.2). MRI wasrepeated after exposure to the NBO and HBO at different time points (0 min, 25 min, 50 min). Signal intensities in T1-weighted, T2-weighted images and fluid attenuated inversion recovery (FLAIR) signal intensities of several intracranialstructures were compared between NBO and HBO.Results. Increased T1-weighted signal intensities were observed in white and deep grey brain matter, cerebrospinalfluid (CSF), venous blood and vitreous body after exposure to NBO as well as to HBO compared to baseline (Dunnett’stest, p < 0.05) without significant differences between both protocols. There was also no significant difference in T2-weighted signal intensities between NBO and HBO. FLAIR signal intensities were increased only in the vitreous bodyafter NBO and HBO and FLAIR signal of caudate nucleus was decreased after NBO (Dunnett’s test, p < 0.05). Thestatistically significant differences in FLAIR signal intensities were found between NBO and HBO (paired t-test, p < 0.05)in most observed brain structures (paired t-test, p < 0.05).Conclusions. Our results show that NBO and HBO alters signal intensities T1-weighted and FLAIR images of humanbrain. The differences between NBO and HBO are most pronounced in FLAIR imaging. Ključne besede: hyperbaric oxygen, normobaric oxygen, magnetic resonance, human brain Objavljeno v DiRROS: 25.07.2024; Ogledov: 200; Prenosov: 89 Celotno besedilo (1008,50 KB) |
5. Precision transcriptomics of viral foci reveals the spatial regulation of immune-signaling genes and identifies RBOHD as an important player in the incompatible interaction between potato virus Y and potatoTjaša Lukan, Maruša Pompe Novak, Špela Baebler, Magda Tušek-Žnidarič, Aleš Kladnik, Maja Križnik, Andrej Blejec, Maja Zagorščak, Katja Stare, Barbara Dušak, Anna Coll Rius, Stephan Pollmann, Karolina Morgiewicz, Jacek Hennig, Kristina Gruden, 2020, izvirni znanstveni članek Povzetek: Whereas the activation of resistance (R) proteins has been intensively studied, the downstream signaling mechanisms leading to the restriction of the pathogen remain mostly unknown. We studied the immunity network response conditioned by the potato Ny-1 gene against potato virus Y. We analyzed the processes in the cell death zone and surrounding tissue on the biochemical and gene expression levels in order to reveal the spatiotemporal regulation of the immune response. We show that the transcriptional response in the cell death zone and surrounding tissue is dependent on salicylic acid (SA). For some genes the spatiotemporal regulation is completely lost in the SA-deficient line, whereas other genes show a different response, indicating multiple connections between hormonal signaling modules. The induction of NADPH oxidase RBOHD expression occurs specifically on the lesion border during the resistance response. In plants with silenced RBOHD, the functionality of the resistance response is perturbed and the spread of the virus is not arrested at the site of infection. RBOHD is required for the spatial accumulation of SA, and conversely RBOHD is under the transcriptional regulation of SA. Using spatially resolved RNA-seq, we also identified spatial regulation of an UDP-glucosyltransferase, another component in feedback activation of SA biosynthesis, thus deciphering a novel aspect of resistance signaling. Ključne besede: immune signaling network, NADPH oxidase RBOHD, reactive oxygen species, salicylic acid, Sola-num tuberosum (potato), spatiotemporal response analysis, virus resistance, Potyvirus Objavljeno v DiRROS: 22.07.2024; Ogledov: 207; Prenosov: 155 Celotno besedilo (2,87 MB) Gradivo ima več datotek! Več... |
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7. Inflammatory, oxidative stress and small cellular particle response in HUVEC induced by debris from endoprosthesis processingZala Jan, Matej Hočevar, Veno Kononenko, Sara Michelini, Neža Repar, Maja Caf, Boštjan Kocjančič, Drago Dolinar, Slavko Kralj, Darko Makovec, Aleš Iglič, Damjana Drobne, Monika Jenko, Veronika Kralj-Iglič, 2023, izvirni znanstveni članek Ključne besede: endoprosthesis failure, corundum, cytokines, reactive oxygen species, lipid droplets Objavljeno v DiRROS: 02.02.2024; Ogledov: 566; Prenosov: 227 Celotno besedilo (4,34 MB) Gradivo ima več datotek! Več... |
8. Solidification behavior of Fe-6.5Si alloy powder for AM-SLM processing, as assessed by differential scanning calorimetryDarja Steiner Petrovič, Črtomir Donik, Irena Paulin, Matjaž Godec, Maja Vončina, Martin Petrun, 2023, izvirni znanstveni članek Ključne besede: soft magnetic alloy, differential scanning calorimetry, oxygen, high-temperature phase, microstructure, eutectics Objavljeno v DiRROS: 02.02.2024; Ogledov: 465; Prenosov: 210 Celotno besedilo (9,05 MB) Gradivo ima več datotek! Več... |
9. High sensitivity measurements of gas transport through films for food packaging and the ▫$O_2$▫ adsorption issueMarine Schott, Janez Šetina, Espedito Vassallo, Matteo Pedroni, Roberta Campardelli, Patrizia Perego, Luca Repetto, Giuseppe Firpo, 2023, izvirni znanstveni članek Ključne besede: oxygen transmission rate, food packaging, gas permeability, O2adsorption Objavljeno v DiRROS: 26.01.2024; Ogledov: 382; Prenosov: 225 Celotno besedilo (2,56 MB) Gradivo ima več datotek! Več... |
10. First experience with a novel luminescence-based optical sensor for measurement of oxygenation in tumorsTomaž Jarm, Hotimir Lešničar, Gregor Serša, Damijan Miklavčič, 2001, izvirni znanstveni članek Povzetek: Background. The purpose of this preliminary study was to evaluate a novel luminescence-based fiber-optic sensor (OxyLite system) for the measurement of partial pressure of oxygen (pO2) in tumors and for the detection of changes inpO2 as a function of time. The new method was used simultaneously with the laser Doppler flowmetry method for the measurement of relative tissue perfusion. Materials and methods. Blood perfusion and pO2 were measured continuously via fiber-optic sensors inserted into SA-1 tumors in anesthetizedAžJ mice. The changes in blood flow and oxygenation of tumors wereinduced by transient changes of the parameters of anesthesia and by injection of a vasoactive drug hydralazine. Results. Both optical methods usedin the study successfully detected the induced changes in blood flow and pO2. The measurements of pO2 were well correlated with measurements of microcirculatory blood perfusion. In the majority of pO2 measurements, we observed an unexpected behavior of the signal during the stabilization processimmediately after the insertion of the probe into tumor. This behaviourof the pO2 signal was most probably caused by local tissue damage induced by the insertion of the probe. Conclusion. The novel luminescence-based optical oximetry can reliably detect local pO2 changes in tumors as a function of time but some aspects of prolonged pO2 measurement by this method require further investigation. Ključne besede: sarcoma experimental, blood supply, laser-doppler flowmetry, oxygen, luminiscence Objavljeno v DiRROS: 25.01.2024; Ogledov: 435; Prenosov: 123 Celotno besedilo (231,19 KB) |