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Iskalni niz: "ključne besede" (molecular identification) .

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Description, identification, and growth of Tuber borchii Vittad. mycorrhized Pinus sylvestris L. seedlings on different lime contents
Tanja Mrak, Tine Grebenc, Silke Friedrich, Babette Münzenberger, 2024, izvirni znanstveni članek

Povzetek: Tuber borchii forms ectomycorrhiza with oaks, hazel, and pines, including Pinus sylvestris. However, its ectomycorrhiza morphotype with P. sylvestris was not comprehensively described so far, and molecular analyses are missing despite a high danger of misidentification of T. borchii ectomycorrhiza with other closely related and less valuable truffle species. We described for the first time the morphology and anatomy of T. borchii-P. sylvestris ectomycorrhiza using differential interference contrast technique and semi-thin sections in combination with molecular confirmation of identity. Color of ectomycorrhiza is reddish to dark brown, and morphotypes are unevenly but densely covered by warts-bearing pin-like cystidia. All layers of the hyphal mantle are pseudoparenchymatous with outer mantle layer formed of epidermoid cells. T. borchii ectomycorrhiza was identified by a molecular comparison with fruitbodies used for inoculation and its respective ectomycorrhizae. T. borchii has a wide ecological amplitude. To get a better insight in mycorrhization requirements, we investigated growth of P. sylvestris and its ectomycorrhiza infection rate with T. borchii in substrate with different lime content. The mycorrhization of P. sylvestris with T. borchii in the mycorrhization substrate and cultivation in greenhouse conditions was successful, with colonization of P. sylvestris varying between 36.5 and 48.1%. There was no significant correlation of mycorrhization to applied lime contents, and consequently to pH in substrate, while the increased levels of lime improved growth of the P. sylvestris seedlings.
Ključne besede: root anatomy, ectomycorrhiza, Limestone content, molecular identification, Pinus sylvestris, Tuber borchii
Objavljeno v DiRROS: 22.01.2024; Ogledov: 189; Prenosov: 92
.pdf Celotno besedilo (1,30 MB)
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Ectomycorrhizal fungal community in mature white poplar plantation
Marina Milović, Saša Orlović, Tine Grebenc, Marko Bajc, Branislav Kovačević, Hojka Kraigher, 2021, izvirni znanstveni članek

Povzetek: Ectomycorrhizal communities are rarely studied on seasonal basis, especially in poplar plantations. In this study we analysed the ectomycorrhizal community in a mature twenty-year-old white poplar (Populus alba L.) plantation during four consecutive seasons. Using morpho-anatomical and molecular identification 30 taxa of ectomycorrhizal fungi were recorded of which 15 were identified to the species level, 12 to the genus level, 2 to the family, and one morphotype of ectomycorrhizae remained unidentified. The most abundant among identified ectomycorrhizal fungi were: Inocybe griseovelata, Inocybe splendens, Tuber rufum, and Tomentella sp. 2, which together represented up to 50% of all ectomycorrhizal root tips. The number of ectomycorrhizal fungal taxa and the percentage of vital ectomycorrhizal root tips were highest in winter and spring, respectively. The diversity indices of ectomycorrhizae, number of vital ectomycorrhizal root tips, and total fine roots in the studied poplar plantation did not differ between seasons. Ectomycorrhizal fungi belonging to Inocybaceae family and the short-distance exploration strategy were dominant in all four seasons. On the other hand, the abundance of ectomycorrhizal root tips belonging to the medium-distance exploration strategy type was significantly higher in spring in comparison with autumn and winter.
Ključne besede: Populus alba L., Ectomycorrhizal diversity, morpho-anatomical characterization, molecular identification, seasons
Objavljeno v DiRROS: 03.12.2021; Ogledov: 825; Prenosov: 375
.pdf Celotno besedilo (847,82 KB)
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Eutypella parasitica and other frequently isolated fungi in wood of dead branches of young Sycamore Maple (Acer pseudoplatanus) in Slovenia
Ana Brglez, Barbara Piškur, Nikica Ogris, 2020, izvirni znanstveni članek

Povzetek: Eutypella parasitica R.W. Davidson and R.C. Lorenz is the causative agent of Eutypella canker of maple, a destructive disease of maples in Europe and North America. The fungus E. parasitica infects the trunk through a branch stub or bark wound. Because the fungal community may have an impact on infection and colonization by E. parasitica, the composition of fungi colonizing wood of dead branches of sycamore maple (Acer pseudoplatanus L.) was investigated in five sampling sites in Slovenia. Forty samples from each sampling site were collected between the November 2017 and March 2018 period. Isolations were made from the wood in the outer part of dead branches and from discoloured wood in the trunk that originated from a dead branch. Pure cultures were divided into morphotypes, and one representative culture per morphotype was selected for further molecular identification. From a total of 2700 cultured subsamples, 1744 fungal cultures were obtained, which were grouped into 212 morphotypes. The investigated samples were colonized by a broad spectrum of fungi. The most frequently isolated species were Eutypa maura (Fr.) Sacc., Eutypa sp. Tul. and C. Tul., Fusarium avenaceum (Fr.) Sacc., Neocucurbitaria acerina Wanas., Camporesi, E.B.G. Jones and K.D. Hyde and E. parasitica. In this study, we distinguished species diversity and the fungal community. There were no significant differences in the diversity of fungal species between the five sampling sites, and branch thickness did not prove to be a statistically significant factor in fungal species diversity. Nevertheless, relatively low Jaccard similarity index values suggested possible differences in the fungal communities from different sampling sites. This was confirmed by an analysis of similarities, which showed that the isolated fungal community distinctly differed between the five sampling sites and between the different isolation sources. Eutypella parasitica was isolated from all five investigated sampling sites, although Eutypella cankers were observed in only three sampling sites, indicating the possibility of asymptomatic infection.
Ključne besede: fungal composition, invasive species, diversity, fungal communities, molecular identification, frequencies, Jaccard similarity index, Eutypella parasitica, analysis of similarities, colonization
Objavljeno v DiRROS: 26.05.2020; Ogledov: 2054; Prenosov: 1215
.pdf Celotno besedilo (937,18 KB)
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