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EUROHELICAN—The first helicobacter pylori screen-and-treat population-based study in young adults in Europe
Bojan Tepeš, Tatjana Kofol-Bric, Jernej Završnik, Mitja Oblak, Marcel Kralj, Alja Polajžer, Helena Blažun Vošner, Nataša Maguša Lorber, Jin Young Park, Tamara Matysiak‐Budnik, 2026, izvirni znanstveni članek

Povzetek: Background Most gastric cancer cases are attributable to chronic Helicobacter pylori (H. pylori) infection and can theoretically be prevented. Objective In the EUROHELICAN project, we aimed to assess the feasibility, acceptability, effectiveness, and adverse events of a Helicobacter pylori screen-and-treat program in the 30–34-year age group for the first time in Europe. Design The study was conducted in the Community Healthcare Center dr. Adolf Drolc Maribor, following the methodology prepared by the National Institute of Public Health. We invited asymptomatic individuals aged 30–34 to be tested for H. pylori IgG antibodies; positive results were confirmed by urea breath test (UBT). Results 2102 participants accepted the invitation. The response rate was 24.4% (95% CI: 23.2–25.5), which was higher in women—28.1% (95% CI: 26.4–29.8) than in men—20.5% (95% CI: 0.19.0–22.1, p < 0.001). The serological prevalence of H. pylori infection was 14.2% (95% CI: 12.7–15.9). A confirmatory urea breath test (UBT) was positive in 83.7% of serology-positive patients. The eradication rate of 14-day bismuth-based quadruple therapy (esomeprazole 40 mg BID, amoxicillin 500 mg, metronidazole 400 mg and bismuth oxide 120 mg, all QID) was 94.7% (95% CI: 89.5–97.9). Adverse events (AEs) during treatment were reported more frequently in women (38.8%, 95% CI: 28.1–50.3) than in men (21.5%, 95% CI: 12.3–33.5; p < 0.05). Conclusion H. pylori screening and-treat program in 30–34 age group in Slovenia is feasible; H. pylori treatment is very effective with acceptable rate of AEs. Different approaches to raising public awareness are needed to increase participation rates.
Objavljeno v DiRROS: 17.03.2026; Ogledov: 297; Prenosov: 230
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Helicobacter pylori screen-and-treat programs for gastric cancer prevention : IARC working group report
Jin Young Park, Bojan Tepeš, Helena Blažun Vošner, 2026, izvirni znanstveni članek

Povzetek: The IARC Working Group summarizes guidance on H. pylori screen-and-treat programs for gastric cancer prevention and concludes that prevention is the most effective strategy amid a rising global burden.
Objavljeno v DiRROS: 17.03.2026; Ogledov: 328; Prenosov: 135
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Current transformer status online monitoring platform based on big data
Xurong Jin, Xinrui Zhang, Yunpeng Li, 2025, ni določena

Objavljeno v DiRROS: 28.01.2026; Ogledov: 434; Prenosov: 0

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Comparative simulative analysis and design of single-chain self-assembled protein cages
Fei Xiao, Longfei Luo, Xin Liu, Ajasja Ljubetič, Nengzhi Jin, Roman Jerala, Guang Hu, 2024, izvirni znanstveni članek

Povzetek: Coiled-coil protein origami (CCPO) is a modular strategy for the de novo design of polypeptide nanostructures. It represents a type of modular design based on pairwise-interacting coiled-coil (CC) units with a single-chain protein programmed to fold into a polyhedral cage. However, the mechanisms underlying the self-assembly of the protein tetrahedron are still not fully understood. In the present study, 18 CCPO cages with three different topologies were modeled in silico. Then, molecular dynamics simulations and CC parameters were calculated to characterize the dynamic properties of protein tetrahedral cages at both the local and global levels. Furthermore, a deformed CC unit was redesigned, and the stability of the new cage was significantly improved.
Ključne besede: chemical structure, conformation, genetics oligomers stability
Objavljeno v DiRROS: 03.10.2024; Ogledov: 1254; Prenosov: 797
.pdf Celotno besedilo (2,23 MB)
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Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes
Lenny Ferrer, Melanie Mindt, Maria Suarez-Diez, Tatjana Jilg, Maja Zagorščak, Jin-Ho Lee, Kristina Gruden, Volker F. Wendisch, Katarina Cankar, 2022, izvirni znanstveni članek

Povzetek: Indole is produced in nature by diverse organisms and exhibits a characteristic odor described as animal, fecal, and floral. In addition, it contributes to the flavor in foods, and it is applied in the fragrance and flavor industry. In nature, indole is synthesized either from tryptophan by bacterial tryptophanases (TNAs) or from indole-3-glycerol phosphate (IGP) by plant indole-3-glycerol phosphate lyases (IGLs). While it is widely accepted that the tryptophan synthase α-subunit (TSA) has intrinsically low IGL activity in the absence of the tryptophan synthase β-subunit, in this study, we show that Corynebacterium glutamicum TSA functions as a bona fide IGL and can support fermentative indole production in strains providing IGP. By bioprospecting additional bacterial TSAs and plant IGLs that function as bona fide IGLs were identified. Capturing indole in an overlay enabled indole production to titers of about 0.7 g L–1 in fermentations using C. glutamicum strains expressing either the endogenous TSA gene or the IGL gene from wheat.
Ključne besede: Corynebacterium glutamicum, indole, indole-3-glycerol phosphate lyase, tryptophan synthase α-subunit, bioprospecting, fermentative production
Objavljeno v DiRROS: 16.07.2024; Ogledov: 1178; Prenosov: 741
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RILEM TC 247-DTA round robin test : sulfate resistance, alkali-silica reaction and freeze-thaw resistance of alkali-activated concretes
Frank Winnefeld, Gregor J. G. Gluth, Susan A. Bernal, Maria Chiara Bignozzi, Lorenza Carabba, Sundararaman Chithiraputhiran, Alireza Dehghan, Sabina Dolenec, Katja Dombrowski-Daube, Ashish Dubey, Vilma Ducman, Yu Jin, Karl Peterson, Stephen Dietmar, John L. Provis, 2020, izvirni znanstveni članek

Povzetek: The RILEM technical committee TC 247-DTA ‘Durability Testing of Alkali-Activated Materials’ conducted a round robin testing programme to determine the validity of various durability testing methods, originally developed for Portland cement based-concretes, for the assessment of the durability of alkali-activated concretes. The outcomes of the round robin tests evaluating sulfate resistance, alkali-silica reaction (ASR) and freeze–thaw resistance are presented in this contribution. Five different alkali-activated concretes, based on ground granulated blast furnace slag, fly ash, or metakaolin were investigated. The extent of sulfate damage to concretes based on slag or fly ash seems to be limited when exposed to an Na2SO4 solution. The mixture based on metakaolin showed an excessive, very early expansion, followed by a dimensionally stable period, which cannot be explained at present. In the slag-based concretes, MgSO4 caused more expansion and visual damage than Na2SO4; however, the expansion limits defined in the respective standards were not exceeded. Both the ASTM C1293 and RILEM AAR-3.1 test methods for the determination of ASR expansion appear to give essentially reliable identification of expansion caused by highly reactive aggregates. Alkali-activated materials in combination with an unreactive or potentially expansive aggregate were in no case seen to cause larger expansions; only the aggregates of known very high reactivity were seen to be problematic. The results of freeze–thaw testing (with/without deicing salts) of alkali-activated concretes suggest an important influence of the curing conditions and experimental conditions on the test outcomes, which need to be understood before the tests can be reliably applied and interpreted.
Ključne besede: alkali-activated materials/geopolymers, sulphate resistance, alkali silica reactivity, freeze-thaw resistance, Rilem TC
Objavljeno v DiRROS: 17.08.2023; Ogledov: 3020; Prenosov: 1149
.pdf Celotno besedilo (560,56 KB)
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