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Iskalni niz: "avtor" (Michael P. O'Connell) .

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1.
Comparative study of interactions of aliskiren and AT [sub] 1 receptor antagonists with lipid bilayers
Gregor Mali, Eleni Siapi, M. Papadopoulos, Grigorios Megariotis, Kyriakos Viras, Petros Chatzigeorgioug, Dimitrios Ntountaniotis, Michael Rappolt, A. Sadegphour, Thomas Michael Mavromoustakos, 2015

Povzetek: The renin-angiotensin-aldosterone system (RAAS) plays a key role in the regulation of blood pressure. Renin is the rate limiting enzyme of the RAAS and aliskiren is a highly potent and selective inhibitor of the human renin. Renin is known to be active both in the circulating blood stream as well as locally, when bound to the (pro)-renin receptor ((P)RR). In this study we have investigated a possible mechanism of action of aliskiren, in which its accumulation in the plasma membrane is considered as an essential step for effective inhibition. Aliskiren's interactions with model membranes (cholesterol rich and poor) have been investigated by applying different complementary techniques: differential scanning calorimetry (DSC), Raman spectroscopy, magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy and small- and wide-angle X-ray scattering (SAXS and WAXS). In addition, in silico molecular dynamics (MD) calculations were applied for further confirmation of the experimental data. Aliskiren's thermal effects on the pre- and main transition of dipalmitoyl-phosphatidylcholine (DPPC) membranes as well as its topographical position in the bilayer show striking similarities to those of angiotens.in II type 1 receptor (AT1R) antagonists. Moreover, at higher cholesterol concentrations aliskiren gets expelled from the membrane just as it has been recently demonstrated for the angiotensin receptor blocker (ARB) losartan. Thus, we propose that both the AT1R and the (P)RR-bound renin active sites can be efficiently blocked by membrane-bound ARBs and aliskiren when cholesterol rich membrane rafts/caveolae are formed in the vicinity of the receptors.
Ključne besede: aliskiren, renin, PRR, DPPC bilayers, raman spectroscopy, solid state NMR spectroscopy, SAXS and WAXS, MD simulations
DiRROS - Objavljeno: 26.01.2015; Ogledov: 3227; Prenosov: 103

2.
Dendrochronological dating of two violins from private collections in Slovenia
Katarina Čufar, Micha Beuting, Michael Grabner, 2010

Povzetek: Dendrochronological analyses were made in two violins from private collectionsin Slovenia with an aim to date the wood of their resonance boards,to assess the time of their fabrication, to define the provenance of the wood, and to establish whether the labels of the instruments were original. The origin, the history and the exact age of the instruments had previously not been known. Tree-ring measurements were done on the surface of the bellies. In violin 1, it was made of one radial board, in violin 2 of two radial boards of Norway spruce (Picea abies). In violin 1, we measured 248 tree-rings and the year of the youngest ring was 1808. The dating was confirmed with more than 20 tree-ring chronologies from Austria, Germany, and Italy. The maximal value of the cross-dating parameter t-value after Hollstein(TH) was 12.4. In violin 2, we measured 141 and 137 tree-rings on each side of the belly, and the year of the youngest ring was 1640. The dating was also obtained by cross-dating with over 20 chronologies and confirmed with statistically significant TH up to 9.2. The year of the last ring in both cases corresponded with the terminus post quem, which indicated that the belly (instrument) was fabricated after the year of formation of the youngest ring. There is no evidence about the duration of seasoning and storage of the lumber, or how many tree-rings were removed when the instrumentwas manufactured. Our dendro-provenance study showed that the violin 1 most likely originated from Austria or southern Germany. The wood for the belly of the violin 2 possibly originated from Austria and the sequence significantly matched a chronology built from the instruments made by Jacob Stainer. In both instruments, the dendrochronological dating did not confirm the inscriptions on the labels on the inside of the instrument. The presented investigation was performed in 2008 and 2009 and is to our knowledge the first dendrochronological dating of music instruments in Slovenia.
DiRROS - Objavljeno: 12.07.2017; Ogledov: 1243; Prenosov: 357
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3.
Zusammenarbeit Österreich-Slowenien auf dem gebiet der waldbodenkunde
Franz Mutsch, Michael Englisch, 1992

Ključne besede: tla, gozdovi, raziskovalne metode
DiRROS - Objavljeno: 12.07.2017; Ogledov: 1737; Prenosov: 426
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Heritable risk for severe anaphylaxis associated with increased [alpha]-tryptase-encoding germline copy number at TPSAB1
Jonathan J. Lyons, Jack Chovanec, Michael P. O'Connell, Yihui Liu, Julij Šelb, Roberta Zanotti, Yun Bai, Jiwon Kim, Quang T. Le, Tom DiMaggio, Matija Rijavec, Peter Korošec, 2020

Povzetek: Background: An elevated basal serum tryptase level is associated with severe systemic anaphylaxis, most notably caused by Hymenoptera envenomation. Although clonal mast cell disease is the culprit in some individuals, it does not fully explain this clinical association. Objective: Our aim was to determine the prevalence and associated impact of tryptase genotypes on anaphylaxis in humans. Methods: Cohorts with systemic mastocytosis (SM) and venom as well as idiopathic anaphylaxis from referral centers in Italy, Slovenia, and the United States, underwent tryptase genotyping by droplet digital PCR. Associated anaphylaxis severity (Mueller scale) was subsequently examined. Healthy volunteers and controls with nonatopic disease were recruited and tryptase was genotyped by droplet digital PCR and in silico analysis of genome sequence, respectively. The effects of pooled and recombinant human tryptases, protease activated receptor 2 agonist and antagonist peptides, and a tryptase-neutralizing mAb on human umbilical vein endothelial cell permeability were assayed using a Transwell system. Results: Hereditary [alpha]-tryptasemia (H[alpha]T)--a genetic trait caused by increased [alpha]-tryptase-encoding Tryptase-[alpha]/[beta]1 (TPSAB1) copy number resulting in elevated BST level--was common in healthy individuals (5.6% [n = 7 of 125]) and controls with nonatopic disease (5.3% [n = 21 of 398]). H[alpha]T was associated with grade IV venom anaphylaxis (relative risk = 2.0; P < .05) and more prevalent in both idiopathic anaphylaxis (n = 8 of 47; [17%; P = .006]) and SM (n = 10 of 82 [12.2%; P = .03]) relative to the controls. Among patients with SM, concomitant H[alpha]T was associated with increased risk for systemic anaphylaxis (relative risk = 9.5; P = .007). In vitro, protease-activated receptor-2-dependent vascular permeability was induced by pooled mature tryptases but not [alpha]- or [beta]-tryptase homotetramers. Conclusions: Risk for severe anaphylaxis in humans is associated with inherited differences in [alpha]-tryptase-encoding copies at TPSAB1.
Ključne besede: mastocytosis, venoms, hypersensitivity, anaphylaxis - diagnosis, mast cells, idiopathic anaphylaxis, mast cell activation, hereditary alpha-tryptasemia
DiRROS - Objavljeno: 11.09.2020; Ogledov: 468; Prenosov: 63

7.
Worldwide perspectives on venom allergy
Peter Korošec, Thilo Jakob, Harfi Harb, Robert Heddle, Sarah Karabus, Ricardo de Lima Zollner, Julij Šelb, Bernard Yu-Hor Thong, Fares Zaitoun, David B. K. Golden, Michael Levin, 2019

Povzetek: Venom immunotherapy is the standard of care for people with severe reactions and has been proven to reduce risk of future anaphylactic events. There is a moral imperative to ensure production, supply and worldwide availability of locally relevant, registered, standardized commercial venom extracts for diagnosis and treatment. Insects causing severe immediate allergic reactions vary by region worldwide. The most common culprits include honeybees (Apis mellifera), social wasps including yellow jackets (Vespula and Dolichovespula), paper wasps (Polistes) and hornets (Vespa), stinging ants (Solenopsis, Myrmecia, Pachycondyla, and Pogonomyrmex), and bumblebees (Bombus). Insects with importance in specific areas of the world include the Australian tick (Ixodes holocyclus), the kissing bug (Triatoma spp), horseflies (Tabanus spp), and mosquitoes (Aedes, Culex, Anopheles). Reliable access to high quality venom immunotherapy to locally relevant allergens is not available throughout the world. Many current commercially available therapeutic vaccines have deficiencies, are not suitable for, or are unavailable in vast areas of the globe. New products are required to replace products that are unstandardized or inadequate, particularly whole-body extract products. New products are required for insects in which no current treatment options exist. Venom immunotherapy should be promoted throughout the world and the provision thereof be supported by health authorities, regulatory authorities and all sectors of the health care service.
Ključne besede: allergy and immunology, venoms, Hymenoptera, bee venoms, wasp venoms, insecta, ants hornet, bumblebee, mosquitoes, venom immunotherapy, immunologic desensitization
DiRROS - Objavljeno: 23.09.2020; Ogledov: 445; Prenosov: 180
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8.
The Chronic Urticaria Registry (CURE) : rationale, methods, and initial implementation
Karsten Weller, Ana M. Giménez-Arnau, Clive Grattan, Riccardo Asero, Pascale Mathelier-Fusade, Mojca Bizjak, Michael Hanna, Marcus Maurer, 2020

Povzetek: Background: Chronic urticaria (CU) is a common disease, characterized by the recurrent appearance of wheals, angioedema, or both for more than 6 weeks. Its underlying biology is not well understood, and many patients do not obtain sufficient relief from recommended treatments. Patient registries are rapidly growing as a form of research, because they can provide powerful, data-driven insights about the epidemiology of diseases, real-world effectiveness of treatments, rare patient types, safety monitoring, healthcare costs, and opportunities for quality improvement of healthcare delivery. Objectives: The Chronic Urticaria Registry (CURE) has been designed to improve the scientific understanding, clinical treatment, and healthcare planning of chronic urticaria patients. This report describes the rationale, methods, and initial implementation of this registry. Methods: CURE is an ongoing, prospective, international, multicenter, observational, voluntary registry of patients with CU. Participation in CURE is open to any physician treating CU patients, regardless of location, medical specialty, or type of practice setting. CURE aims to collect data on all CU patients, with no intentional selection or exclusion criteria. It collects baseline and follow-up data on the patient's demographics, history, symptoms, trigger and risk factors, therapies, and healthcare utilization. Results: CURE is a landmark achievement of the global urticaria medical community. As of 26 February 2020, 39 centers around the world have joined the registry and 35 have entered baseline data on a total of 2946 patients. Publications of this data will be forthcoming soon. Conclusions: CURE is eagerly seeking the participation of more physicians and the support of more governmental, charitable, and commercial sponsors from around the world. Here, in this paper, we invite other physicians to join this unique project to improve the lives of patients with CU.
Ključne besede: urticaria, registries
DiRROS - Objavljeno: 07.10.2020; Ogledov: 298; Prenosov: 121
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9.
Comparative epidemiology of suspected perioperative hypersensitivity reactions
Paul Michel Mertes, Didier G. Ebo, Tomaz Garcez, Michael Rose, Vito Sabato, Tomonori Takazawa, Peter J. Cooke, Russel C. Clarke, Pascale Dewachter, Lene Heise Garvey, Anne Berit Guttormsen, David L. Hepner, Philip M. Hopkins, David A. Khan, Peter Kopač, Peter R. Platt, Louise C. Savic, 2019

Povzetek: Suspected perioperative hypersensitivity reactions are rare but contribute significantly to the morbidity and mortality of surgical procedures. Recent publications have highlighted the differences between countries concerning the respective risk of different drugs, and changes in patterns of causal agents and the emergence of new allergens. This review summarises recent information on the epidemiology of perioperative hypersensitivity reactions, with specific consideration of differences between geographic areas for the most frequently involved offending agents.
Ključne besede: anaphylaxis, anti-bacterial agents, blood, chlorhexidine, latex, neuromuscular blocking agents, hypersensitivity, drug hypersensitivity, sugammadex, antibiotics, blood products, perioperative anaphylaxis, perioperative hypersensitivity
DiRROS - Objavljeno: 16.10.2020; Ogledov: 383; Prenosov: 70

10.
Ethical dilemmas when using citizen science for early detection of invasive tree pests and diseases
Erin Bullas-Appleton, Mariella Marzano, Michael J.O. Pocock, Alison Dyke, Maarten De Groot, Craig Shuttleworth, Rehema White, 2020

Povzetek: The early detection of tree health pests and disease is an important component of biosecurity to protect the aesthetic, recreational and economic importance of trees, woodlands and forestry. Citizen science is valuable in supporting the early detection of tree pests and diseases. Different stakeholders (government, business, society and individual) will vary in their opinion of the balance between costs and benefits of early detection and consequent management, partly because many costs are local whereas benefits are felt at larger scales. This can create clashes in motivations of those involved in citizen science, thus leading to ethical dilemmas about what is good and responsible conduct for the use of citizen science. We draw on our experience of tree health citizen science to exemplify five dilemmas. These dilemmas arise because: the consequences of detection may locally be severe (e.g. the destruction of trees); knowledge of these impacts could lead to refusal to make citizen science reports; citizen science reports can be made freely, but can be costly to respond to; participants may expect solutions even if these are not possible; and early detection is (by definition) a rare event. Effective engagement and dialogue across stakeholders, including public stakeholders, is important to properly address these issues. This is vital to ensure the public%s long-term support for and trust in the use of citizen science for the early detection of tree pests and diseases.
Ključne besede: alien species, volunteer, eradication, participatory research
DiRROS - Objavljeno: 14.12.2020; Ogledov: 192; Prenosov: 70
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