1. Metadata from: Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal regionAnže Vozelj, Tjaša Mahkovec Povalej, Katja Stare, Magda Tušek-Žnidarič, Katarina Bačnik, Valentina Levak, Ion Gutiérrez-Aguirre, Marjetka Podobnik, Kristina Gruden, Anna Coll Rius, Tjaša Lukan, 2025, zaključena znanstvena zbirka raziskovalnih podatkov Ključne besede: potato virus Y, coat protein, viral movement, point mutations, Solanum tuberosum
Objavljeno v DiRROS: 31.03.2026; Ogledov: 227; Prenosov: 202
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2. Supplementary material: microbiome analysis of groundwaters in the Slovenian Dinaric Karst, a recognized biodiversity hotspot : version v1Katarina Bačnik, Cene Gostinčar, Rok Kostanjšek, Janez Mulec, Monika Novak Babič, Ion Gutiérrez-Aguirre, Nina Gunde-Cimerman, Martina Turk, Denis Kutnjak, 2026, zaključena znanstvena zbirka raziskovalnih podatkov Ključne besede: Karst cave waters, high-throughput sequencing, viruses, archaea, bacteria, fungi, protozoa
Objavljeno v DiRROS: 24.03.2026; Ogledov: 208; Prenosov: 239
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3. Microbiome analysis of groundwaters in the Slovenian Dinaric Karst, a recognized biodiversity hotspotKatarina Bačnik, Denis Kutnjak, Cene Gostinčar, Rok Kostanjšek, Janez Mulec, Monika Novak Babič, Ion Gutiérrez-Aguirre, Nina Gunde-Cimerman, Martina Turk, 2026, izvirni znanstveni članek Povzetek: Groundwater is the most important source of drinking water supply, irrigation, and industrial use, as well as a habitat for a diverse range of (micro)organisms. Some of the first studies of underground environments were carried out in the Dinaric Karst region of Slovenia, which harbors one of the highest subterranean biodiversities in the world. Despite their ecological importance, groundwater microbial communities remain underexplored. In this study, we conducted a comprehensive microbiome analysis of karst groundwater, investigating the abundance and diversity of viruses, archaea, bacteria, fungi, and protozoa. We detected a high diversity of both prokaryote- and eukaryote-infecting viruses, indicating the connection of subterranean environments with surface waters. In the archaeal community, seven different phyla were identified, dominated by members of "Candidatus Woesearchaeota" and Nitrososphaerota (syn. Thermoproteota). In the bacterial community of majority of the samples, the Pseudomonadota was the most abundant phylum, followed by Bacteroidota, Actinomycetota, and Verrucomicrobiota. Fungal abundance and diversity differed greatly between cave water samples, with the Ascomycota families Cladosporiaceae, Didymellaceae, Aspergillaceae and saprotrophic Basidiomycota detected in all samples. Besides fungi, microscopic eukaryotic diversity consisted of ciliates, copepods and golden-brown algae present in all samples. Differences in microbial communities between the sampling sites highlight the need for tailored site-specific groundwater conservation and monitoring strategies. Moreover, the identification of microbes associated with anthropogenic activities underscores their potential as source-specific indicators of groundwater pollution. As the first holistic overview of microbial diversity of subterranean water, the study establishes a valuable baseline for future research and conservation of these unique karst ecosystems.
Ključne besede: Karst cave water, high-throughput sequencing, viruses, archaea, bacteria, fungi, protozoa
Objavljeno v DiRROS: 02.03.2026; Ogledov: 318; Prenosov: 198
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4. Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal regionAnže Vozelj, Tjaša Mahkovec Povalej, Katja Stare, Magda Tušek-Žnidarič, Katarina Bačnik, Valentina Levak, Ion Gutiérrez-Aguirre, Marjetka Podobnik, Kristina Gruden, Anna Coll Rius, Tjaša Lukan, 2025, izvirni znanstveni članek Povzetek: Potato virus Y (PVY) is one of the top 10 economically most important plant viruses and responsible for major yield losses. We previously suggested the involvement of the N-terminal region of PVY coat protein (CP) in PVY spread. By constructing different N-terminal deletion mutants of the PVY N605 strain, we here show that deletions of 40 or more amino acid residues from the N-terminal region of the CP resulted in the PVY multiplication limited to primary infected cells in Nicotiana clevelandii plants. Deletion of 26 residues profoundly impaired PVY cell-to-cell movement and prevented systemic PVY spread, while deletions of 19-23 residues allowed delayed systemic PVY spread. Introduced point mutations in the identified region prevent (S21G) or delay (G20P) PVY movement. In summary, this work shows the significance of the CP N-terminus for movement of the PVY.
Ključne besede: potato virus Y, potato, coat proteins, viral movement, point mutations
Objavljeno v DiRROS: 18.12.2025; Ogledov: 1013; Prenosov: 424
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5. Viromska analiza RNA virusov v vzorcih Triglavskega ledenika in ledenika pod SkutoKristina Andrejc, Maša Jarčič, Ion Gutiérrez-Aguirre, Denis Kutnjak, Katarina Bačnik, 2025, izvirni znanstveni članek Povzetek: V ledenikih se kljub nizkim temperaturam in omejeni količini dosegljive vode ter hranil skriva velika biološka raznolikost. V ledeniških mikrobnih skupnostih prevladujejo bakterije, alge in glive. Znanje o virusih v teh ekstremnih okoljih pa je omejeno, kar smo želeli nasloviti z viromsko analizo slovenskih ledenikov: Triglavskega ledenika in ledenika pod Skuto. Iz koncentriranih vzorcev staljenega ledeniškega ledu smo izolirali ribonukleinske kisline (RNA), jih pomnožili z naključnimi oligonukleotidnimi začetniki in netarčno določili njihova zaporedja s platformo Illumina. S pomočjo bioinformatskih analiz smo v pridobljenih podatkih poiskali virusna zaporedja in jih taksonomsko uvrstili. Odstotki odčitkov, ki so se klasificirali kot virusni, so bili pričakovano nizki. Kljub osredotočenosti na opis raznolikosti RNA virusnih zaporedij, smo v vseh vzorcih slovenskih ledenikov najbolj pogosto zaznali virusne odčitke, ki so pripadali DNA bakteriofagom, klasificiranim v svet Duplodnaviria. Virusna zaporedja odkrita v vzorcih slovenskih ledenikov so večinoma izkazovala nizke identitete z znanimi virusnimi zaporedji iz podatkovnih baz, kar kaže na prisotnost številnih novih virusnih vrst. Daljša sestavljena virusna zaporedja verjetno predstavljajo celotne genome novo odkritih RNA virusov (npr. virusna zaporedja iz skupine toti-podobnih in picorna-podobnih virusov). Za taka zaporedja smo s filogenetskimi analizami pokazali, katerim znanim virusom so najbolj sorodni in kakšne organizme potencialno okužujejo. Raziskava viroma slovenskih ledenikov predstavlja prvi vpogled v prisotnost virusov v teh okoljih pri nas in razkriva, da se v njih skriva velika in neraziskana raznolikost teh bioloških entitet.
Ključne besede: virusi, viromska analiza, Triglavski ledenik, ledenik pod Skuto
Objavljeno v DiRROS: 18.11.2025; Ogledov: 434; Prenosov: 376
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6. Odprta znanost : Mala Loža, Koper, od 29. julija do 18. avgusta 2025 in Gallusovo nabrežje, Ljubljana, od 28. avgusta do 6. oktobra 20252025, razstava Ključne besede: odprta znanost, občanska znanost, raziskovalni podatki, podatkovni svetovalec, repozitoriji, projekt SPOZNAJ
Objavljeno v DiRROS: 06.11.2025; Ogledov: 745; Prenosov: 747
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8. Annual (2024) taxonomic update of RNA-directed RNA polymerase-encoding negative-sense RNA viruses (realm Riboviria: kingdom Orthornavirae: phylum Negarnaviricota)Jens H. Kuhn, Scott Adkins, Sergey V. Alkhovsky, Wenxia An, Tatjana Avšič-Županc, María A. Ayllón, Katarina Bačnik, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Ion Gutiérrez-Aguirre, Denis Kutnjak, Nataša Mehle, Anja Pecman, Maja Ravnikar, Ana Vučurović, 2025, izvirni znanstveni članek Povzetek: In April 2024, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was expanded by 1 new order, 1 new family, 6 new subfamilies, 34 new genera and 270 new species. One class, two orders and six species were renamed. Seven families and 12 genera were moved; ten species were renamed and moved; and nine species were abolished. This article presents the updated taxonomy of Negarnaviricota as currently accepted by the ICTV, providing an essential annual update on the classification of members of this phylum that deepen understandings of their evolution, and supports critical public health measures for virus identification and tracking.
Ključne besede: Aliusviridae, Arenaviridae, articulaviral, Articulavirales, Artoviridae, Aspiviridae, Bornaviridae, bunyaviral, Bunyavirales, Bunyaviricetes, bunyavirus, Crepuscuviridae, Discoviridae, Elliovirales, Filoviridae, Fimoviridae, Goujianvirales, Hantaviridae, Hareavirales, International Committee on Taxonomy of Viruses (ICTV), jingchuviral, Jingchuvirales, Konkoviridae, Lispiviridae, megaclassification, megataxonomy, mononegaviral, Mononegavirales, muviral, Muvirales, Mymonaviridae, Myriaviridae, naedreviral, Naedrevirales, Nairoviridae, Natareviridae, negarnaviricot, Negarnaviricota, Nyamiviridae, Orthomyxoviridae, Orthornavirae, Paramyxoviridae, Peribunyaviridae, Phasmaviridae, Phenuiviridae, Pneumoviridae, Rhabdoviridae, Riboviria, Sunviridae, Tenuivirus, Tosoviridae, Tospoviridae, Tulasviridae, virus classification, virus nomenclature, virus taxonomy, virology
Objavljeno v DiRROS: 24.06.2025; Ogledov: 867; Prenosov: 649
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9. One-step RT-droplet digital PCR : a breakthrough in the quantification of waterborne RNA virusesNejc Rački, Dany Morisset, Ion Gutiérrez-Aguirre, Maja Ravnikar, 2014, izvirni znanstveni članek Povzetek: Water contamination by viruses has an increasing worldwide impact on human health, and has led to requirements for accurate and quantitative molecular tools. Here, we report the first one-step reverse-transcription droplet digital PCR-based absolute quantification of a RNA virus (rotavirus) in different types of surface water samples. This quantification method proved to be more precise and more tolerant to inhibitory substances than the benchmarking reverse-transcription real-time PCR (RT-qPCR), and needs no standard curve. This new tool is fully amenable for the quantification of viruses in the particularly low concentrations usually found in water samples.
Ključne besede: waterborne virus, quantification, droplet digital PCR, real-time PCR, digital PCR
Objavljeno v DiRROS: 04.03.2025; Ogledov: 1107; Prenosov: 720
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10. Detection of rare drug resistance mutations by digital PCR in a human influenza A virus model system and clinical samplesAlexandra S. Whale, Claire Bushell, Paul R. Grant, Simon Cowen, Ion Gutiérrez-Aguirre, Denise M. OʹSullivan, Jana Žel, Mojca Milavec, Carole A. Foy, Eleni Nastouli, Jeremy A. Garson, Jim F. Huggett, 2016, izvirni znanstveni članek Ključne besede: digital PCR, human influenza A viruses
Objavljeno v DiRROS: 26.02.2025; Ogledov: 959; Prenosov: 568
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