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1.
Subterranean environments contribute to three-quarters of classified ecosystem services
Stefano Mammola, David Brankovits, Tiziana Di Lorenzo, Isabel R. Amorim, Raluca Ioana Bancila, Adrià Bellvert, Enrico Bernard, Anna Blomberg, Nataša Mori, Maja Zagmajster, 2026, izvirni znanstveni članek

Povzetek: Beneath the Earth’s surface lies a network of interconnected caves, voids, and systems of fissures forming in rocks of sed-imentary, igneous, or metamorphic origin. Although largely inaccessible to humans, this hidden realm supports and reg-ulates services critical to ecological health and human well-being. Subterranean ecosystems are integral to majorbiogeochemical cycles, sustain diverse surface habitats, and serve as the primary source of irrigation and drinking water.They also offer non-material benefits, including scientific discovery, education, and cultural practices. Yet, these contri-butions often go unrecognised, partly due to the lack of a unified synthesis of ecosystem services across terrestrial, fresh-water, and marine subterranean compartments. This gap limits effective communication of their value to scientists,practitioners, and the public. Through a systematic expert-based review, we show that subterranean ecosystems contrib-ute to up to 75% of classified ecosystem services. Notably, many of these contributions are described only qualitatively,lacking numerical or economic quantification. Next, we list examples of the main ecosystem services provided by subter-ranean systems to offer a global overview of their multifaceted value and vulnerability to environmental change. Webelieve this synthesis provides researchers and practitioners with concrete examples to communicate more effectivelythe importance of subterranean ecosystems to diverse audiences.
Ključne besede: groundwater, hypogean, nature value, drinking water, food production, biotechnology, geothermal energy, sustainability, ecotourism, cultural heritage
Objavljeno v DiRROS: 02.03.2026; Ogledov: 106; Prenosov: 91
.xlsx Celotno besedilo (70,04 KB)
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2.
Carbon cycling dynamics in the headwater Radovna stream recharged by Lipnik springs, a carbonate catchment in the Julian Alps, Slovenia, based on stable isotope analysis
Tjaša Kanduč, Timotej Verbovšek, Nataša Mori, 2025, izvirni znanstveni članek

Povzetek: Carbon cycling was investigated monthly (July, August, September, October, November, March and May) from July 2023 to May 2024 in the Radovna stream originating from the permanent Lipnik spring in the Julian Alps, Slovenia using isotopic composition of carbon in dissolved inorganic carbon (δ13CDIC) and particulate organic carbon (δ13CPOC). The investigated catchment is composed of massive coarse - crystal dolomite and limestone. Total alkalinity ranged from 2.2 to 2.7mM and is characteristic for carbonate pristine environments. In-situ parameters e.g. dissolved oxygen ranged from 11.0 to 12.0mg/L, pH from 7.9 to 8.1 and specific electrical conductivity from 275 to 318μS/cm, respectively. The values of dissolved oxygen reflect that the water system is well oxygenated. CO2 presented a source of carbon to the atmosphere during all investigated months. Oversaturation with CO2 is 1.01 to 4.1 times of atmospheric value. δ13CDICranged from -11.8 to -9.7‰. This indicates that dissolved inorganic carbon under different discharge conditions mainly originates from the dissolution of carbonates (from 50.3 % to 57.3%), followed by the degradation of organic matter (from 42.8% to 49.7%). Equilibration with atmospheric CO₂ has a negligible impact, ranging from 0.01% to 0.11%. δ13CPOC in river water indicate sources such as plant debris, with δ13CPOC of -29.4‰, and highly degraded soil organic matter, with δ13CPOC of -24.9‰. The δ13CDIC and δ13CPOC values are typical for a forest stream flowing over a limestone substrate. Strong statistical negative significant correlation was obtained between electrical conductivity and water temperature, mass of total suspended solids (mTSS) and pH.
Ključne besede: total alkalinity, stable isotopes, carbon, pCO2, headwater stream, Julian Alps
Objavljeno v DiRROS: 23.12.2025; Ogledov: 802; Prenosov: 104
.pdf Celotno besedilo (3,60 MB)

3.
Are caves enough to represent karst groundwater biodiversity? Insights from geospatial analyses applied to European obligate groundwater-dwelling copepods
Emma Galmarini, Mattia Di Cicco, Barbara Fiasca, Nataša Mori, Mattia Iannella, Tiziana Di Lorenzo, Francesco Cerasoli, Diana Maria Paola Galassi, 2025, izvirni znanstveni članek

Povzetek: Caves are recognized as biodiversity hotspots for groundwater fauna, including obligate groundwater-dwelling copepods (Crustacea: Copepoda), exhibiting high species richness, endemism, and phylogenetic rarity. However, the extent to which caves alone provide a representative estimate of copepod species richness in karst areas remains uncertain. Taking advantage of the recently published EGCop dataset, the first expert-validated, Europe-wide occurrence dataset for obligate groundwater-dwelling copepods (hereinafter, GW copepods), this study investigates the distribution of GW copepods into karst areas, comparing species richness in caves versus other karst groundwater habitats (e.g., springs, karst streams, artificial wells), within and among the European karst units. The main aims are: (i) identifying karst areas which represent hotpots of GW copepod species richness; (ii) assessing to which extent caves, as open windows to the subterranean environments, contribute to define hotspots of GW copepods’ species richness into karst areas across Europe. EGCop comprises 6,986 records from 588 copepod species/subspecies distributed among four orders: Cyclopoida (3,664 records, 184 species), Harpacticoida (3,288 records, 395 species), Calanoida (32 records, seven species), and Gelyelloida (two records, two species). To perform geospatial analyses, we filtered the dataset by: (i) selecting only the records with spatial uncertainty in the associated coordinates lower than 10 km; (ii) searching for those records falling within, or very close to, the polygons representing European karst areas. Species richness hotspots were then estimated through geospatial analyses in geographic information system (GIS) environment. Within the selected records, those specifically referring to karst habitats (2,526 records, 369 species) are primarily represented by Harpacticoida (1,199 records, 228 species) and Cyclopoida (1,293 records, 132 species). Among species collected from karst habitats, records from caves (1,867, 73.9%) belong to 318 species (Harpacticoida = 189, Cyclopoida = 122, Calanoida = 7), representing 86.1% of the total species richness of karst habitats. Geospatial analyses reveal that the European hotspots of GW copepods’ species richness recorded exclusively in caves reflect the spatial arrangement of postglacial refugia in southern karst regions, though representing a subset of the broader diversity found across all karst groundwater habitats. Our findings highlight that the contribution of cave systems in groundwater biodiversity assessments and related conservation planning may vary depending on the evolution and morphologies of the target karst regions—often pointing to a high representativeness of caves for subterranean biodiversity, sometimes revealing their lower explanatory power within the broader karst systems.
Ključne besede: copepoda, groundwater, biodiversity, datasets, caves, karst, Europe
Objavljeno v DiRROS: 19.12.2025; Ogledov: 327; Prenosov: 256
.pdf Celotno besedilo (22,11 MB)
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4.
5.
Subterranean environments contribute to three-quarters of classified ecosystem services
Stefano Mammola, David Brankovits, Tiziana Di Lorenzo, Isabel R. Amorim, Raluca Ioana Bancila, Nataša Mori, Maja Zagmajster, 2025, drugi sestavni deli

Povzetek: Beneath the Earth’s surface lies a network of interconnected caves, voids, and systems of fissures forming in rocks of sedimentary, igneous, or metamorphic origin. Though largely inaccessible to humans, this hidden realm supports and regulates services critical to ecological health and human well-being. Subterranean ecosystems are integral to major biogeochemical cycles, sustain diverse surface habitats, and serve as the primary source of irrigation and drinking water. They also offer non-material benefits, including scientific discovery, education, and cultural practices. Yet, these contributions often go unrecognized, partly due to the lack of a unified synthesis of ecosystem services across terrestrial, freshwater, and marine subterranean compartments. This gap limits effective communication of their value to scientists, practitioners, and the public. Through a systematic expert-based review, we show that subterranean ecosystems contribute to up to 75% of classified ecosystem services. Notably, many of these contributions are described only qualitatively, lacking numerical or economic quantification. Next, we provide examples of the main services to offer a global overview of their multifaceted value and vulnerability to environmental change. We believe this synthesis provides researchers and practitioners with concrete examples and targeted metaphors to more effectively communicate the importance of subterranean ecosystems to diverse audiences.
Ključne besede: cultural heritage, ecotourism, sustainability, geothermal energy, biotechnology, food production, drinking water, nature value, hypogean, groundwater
Objavljeno v DiRROS: 02.07.2025; Ogledov: 667; Prenosov: 997
.pdf Celotno besedilo (1,29 MB)
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6.
A comprehensive occurrence dataset for European Ostracoda inhabiting groundwater and groundwater-dependent ecosystems
Nataša Mori, Živa Vehovar, Traian Brad, Gergely Balázs, Constanze Englisch, Cene Fišer, Santiago Gaviria, Sanja Gottstein Matočec, Christian Griebler, Marius Kenesz, Lee R. F. D. Knight, Florian Malard, Stefano Mammola, Pierre Marmonier, Alejandro Martinez, Maja Zagmajster, 2025, izvirni znanstveni članek

Povzetek: Motivation Groundwater ecosystems sustain a unique and globally important biodiversity but remain understudied due to sampling and exploration challenges, as well as a shortage of taxonomic experts. Groundwater ostracods, like other groundwater taxa, exhibit a high degree of endemism, rarity and subterranean specialisation, positioning them as potentially vulnerable organisms. To better understand biodiversity patterns and the conservation needs of this highly diverse group, we assembled a team of experts to gather the most comprehensive information available about groundwater ostracods in Europe. We present a dataset comprising 2065 occurrence records of 110 species, 11 undescribed species and 5 subspecies of groundwater ostracods. This open dataset may support future research on the distribution, evolutionary pathways and conservation needs of European groundwater ostracods, as well as inspire targeted sampling efforts in regions with currently limited data available. Main Types of Variables Contained Occurrence records of groundwater ostracods, with details about taxonomy, source of records, occurrence locality, habitat type and species dependence on groundwater (obligate [stygobiont] versus facultative groundwater-dwellers [stygophile]). Spatial Location and Grain Geographical Europe, spanning 32 countries. Occurrence records were assigned decimal degrees coordinates (EPSG:4326). Most occurrence records are at 100 m resolution. Time Period 1915–2024. Major Taxa and Level of Measurement Crustacea: Ostracoda. Most records have species or subspecies-level identification, while some are identified to genus or family levels. Software Format Comma-separated values file (.csv) and Excel file (.xlsx), with UTF-8 encoding and metadata provided following the Darwin Core standard.
Ključne besede: alluvial aquifers, conservation, ecotypes, habitat types, hyporheic zone, karst, occurrence data, ostracods, springs
Objavljeno v DiRROS: 20.06.2025; Ogledov: 861; Prenosov: 611
.pdf Celotno besedilo (4,65 MB)
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7.
Spatial and temporal variability of hyporheic invertebrate community within a stream reach of the River Bača (W Slovenia)
Nataša Mori, Anton Brancelj, 2011, izvirni znanstveni članek

Povzetek: We studied spatio-temporal distribution of hyporheic invertebrate community at the stream-reach scale in the River Bača on three sampling occasions (January, March, May) in 2005. On each sampling occasion, invertebrates were collected from the shallow hyporheic zone (RB1; depth 30-60 cm, 3 replicates), and deeper hyporheic zone (RB2; depth 60-90 cm, 2 replicates) in the river bed, and adjacent gravel bar (GB; depth 60-90 cm, 3 replicates) using Bou-Rouch piston pump. Concurrently, temperature, conductivity and oxygen were measured in the surface water and in hyporheic water at each sampling station. Differences in hyporheic community between dates and habitats were analysed by using two-way ANOVA (dates and habitats as fixed factors) and explored by principal component analysis (PCA). Altogether, 21,657 specimens from 63 taxa were collected. Cyclopoida juveniles, Leuctra sp. (Plecoptera), Chironomidae (Diptera), Acanthocyclops vernalis (Fischer, 1853) and Diacyclops languidus (G. O. Sars, 1863) were the most abundant in the samples. Two-way ANOVA showed significant differences between habitats (RB1 and GB), but no differences between dates when using taxonomic richness as dependent variable. No differences between habitats and dates were calculated when invertebrate densities were applied. PCA of hyporheic invertebrate data showed a gradient in community composition from shallow hyporheic zone (RB1) to deeper hyporheic zone (RB2) and gravel bar (GB). The differences were most probably due to different sediment composition in the studied habitats and less frequent disturbances due to floods in deeper layers and lateral gravel bars.
Ključne besede: stream reach, hyporheic zone, invertebrates, distribution (biology), community composition, spatial and temporal distribution
Objavljeno v DiRROS: 12.03.2025; Ogledov: 726; Prenosov: 605
.pdf Celotno besedilo (853,48 KB)
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8.
Contribution to the knowledge on the distribution of Recent free-living freshwater ostracods (Podocopida, Ostracoda, Crustacea) in Slovenia
Nataša Mori, Claude Meisch, 2012, izvirni znanstveni članek

Povzetek: An updated checklist of Recent free-living freshwater ostracods (Podocopida, Ostracoda, Crustacea) from Slovenia together with new records is presented. The new checklist is based on both the records extracted from the literature and the sampling in the field carried out during the last decade. The present checklist comprises 61 species. However, the eastern part of Slovenia and the surface waters are underrepresented. It is expected that the number of species will increase in the future.
Ključne besede: species, fauna, freshwaters, groundwaters
Objavljeno v DiRROS: 12.03.2025; Ogledov: 753; Prenosov: 540
.pdf Celotno besedilo (219,16 KB)
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9.
Interstitial fauna of the Sava River in Eastern Slovenia
Simona Prevorčnik, Anja Remškar, Cene Fišer, Boris Sket, Gregor Bračko, Teo Delić, Nataša Mori, Anton Brancelj, Maja Zagmajster, 2019, izvirni znanstveni članek

Povzetek: Interstitial water that occupies the pore spaces within unconsolidated sediments is a unique habitat, inhabited by surface as well as exclusively subterranean species (stygobionts). The best studied of all interstitial habitats is the hyporheic zone, an interface between surface water and groundwater (phreatic zone) environments. The Sava River in central Slovenia (i.e., at the Ljubljana alluvial plain) readily qualifies as one of the global hotspots of interstitial biodiversity, while data from other river sections are lacking. In 2015, we sampled two gravel bars on the final Slovenian section of the river (Eastern Slovenia), and collected nine samples per site using the Bou-Rouch pumping method. At »Čatež ob Savi« and »Obrežje« sites, at least 26 invertebrate species with 14 stygobionts and 25 invertebrate species with 13 stygobionts were identified, respectively. Altogether, 33 invertebrate taxa with 16 stygobionts were recorded, which increased the previously known interstitial stygobiotic richness of the Sava River in Slovenia by eight species (to 37 species). Three species of the stygobiotic amphipod genus Niphargus were recorded in Slovenia for the first time, one of which is even a new species to science. We discuss the novel results in the context of current national conservation practices.
Ključne besede: interrstitial fauna, hyporheic, stygobionts, endemic species, Bou-Rouch pumping method, Sava River, Slovenia
Objavljeno v DiRROS: 12.03.2025; Ogledov: 760; Prenosov: 553
.pdf Celotno besedilo (459,58 KB)
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10.
An updated checklist of the extant freshwater ostracods (Podocopida, Ostracoda, Crustacea) of Slovenia
Nataša Mori, Ali Šalamun, 2022, izvirni znanstveni članek

Povzetek: The article presents an updated checklist of the extant freshwater ostracods in Slovenia. The data were obtained from the published scientific literature up to 2012 and from field collections after 2012, mainly from springs and groundwaters and, to a lesser extent, from ponds and other surface waters. The erroneous and invalid species names cited in the existing literature are listed to avoid further misquotations. The updated checklist contains a total of 70 valid species names. The species belong to 3 superfamilies, 9 families and 32 genera. The species-richest is the family Candonidae (36 species), followed by the family Cyprididae (22 species). Further field samplings are needed to complete the species list, with the focus on the eastern and southeastern parts of Slovenia, and on the sampling of surface waters. Additionally, more in-depth literature investigations and examinations of old museum collections across Europe need to be carried out to obtain all existing data. Moreover, many stygobiotic species (i.e., species inhabiting exclusively subterranean waters), new for science, collected over the last 20 years, need to be scientifically described. Lastly, the Slovenian National Red list on ostracods needs to be urgently updated.
Ključne besede: Ostracoda, species, distribution, freshwater, groundwater, non-marine
Objavljeno v DiRROS: 04.03.2025; Ogledov: 986; Prenosov: 648
.pdf Celotno besedilo (331,43 KB)
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