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Povzetek: Correlative imaging is a powerful tool for revealing information on cell-type structures and their biochemistry, with the potential to inform healthier food choices and improved dietary recommendations. Determination of plant structures and their structural biochemistry advances our understanding of specific structures designed to store different biomolecules within cells and tissues. Compared to the classical biochemical separation techniques, the key advantage of sequential correlative imaging techniques is in relating spatial plant (micro)structures to their biochemistry in a nondestructive manner. Sequential imaging reported here comprises six methodologies on a single sample, a cross-section of a Tartary buckwheat (Fagopyrum tataricum) grain, namely, bright-field and autofluorescence microscopy, fluorescence microspectroscopy, MeV-secondary ion mass spectrometry, micro-particle-induced X-ray emission, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy, and laser ablation-inductively coupled plasma-mass spectrometry. Results confirm that the stepwise addition of the desired information across several classes of biomolecules and several spatial scales informs the quality and safety of plant-based produce across scales. Therefore, a viable workflow is proposed, enabling sequential spatial analysis of grain and highlighting plant structures' in situ specificity. The advantages and disadvantages of the selected methodologies were critically evaluated.
Ključne besede: autofluorescence, correlative molecular imaging, element distribution, grain tissues
Objavljeno v DiRROS: 07.11.2025; Ogledov: 151; Prenosov: 74
Celotno besedilo (1,71 MB)
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Povzetek: Field production of common beans benefit from root microbial associations, although they are generally considered to be weaker nitrogen fixers than other legumes. Therefore, the number of nodules on the roots of 64 accessions of the European common bean (Phaseolus vulgaris L.) and the relationship between the number of nodules per accession and other plant characteristics (growth type, earliness, seed yield, and total nitrogen content in seeds) were studied. The results indicated that growth type and earliness influenced the number of nodules per accession (the contributions of principal component analysis were 34.9% for Dim1 and 29.8% for Dim2). The average number of nodules per accession with indeterminate growth type was almost five times higher (20.1) than for accessions with determinate growth type (4.4). Common bean accessions with regular growth cycle length had the highest number of nodules (21.9). In contrast, nodulation efficiency, measured as seed yield per plant and total nitrogen content in seeds, were not correlated with the number of nodules per accession (correlation analysis, r < 0.1). Consequently, data on the nodulation efficiency of European common bean accessions are important for breeding programmes in conjunction with other agronomically important traits for commercial and/or organic cultivation systems.
Ključne besede: agronomic traits, common bean, nitrogen fixation, nodules, symbiotic efficiency
Objavljeno v DiRROS: 30.09.2024; Ogledov: 1004; Prenosov: 262
Celotno besedilo (1,36 MB)