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Jellyfish-associated microbiome in the marine environment : exploring its biotechnological potential
Tinkara Tinta, Tjaša Kogovšek, Katja Klun, Alenka Malej, Gerhard J. Herndl, Valentina Turk, 2019, pregledni znanstveni članek

Povzetek: Despite accumulating evidence of the importance of the jellyfish-associated microbiome to jellyfish, its potential relevance to blue biotechnology has only recently been recognized. In this review, we emphasize the biotechnological potential of host–microorganism systems and focus on gelatinous zooplankton as a host for the microbiome with biotechnological potential. The basic characteristics of jellyfish-associated microbial communities, the mechanisms underlying the jellyfish-microbe relationship, and the role/function of the jellyfish-associated microbiome and its biotechnological potential are reviewed. It appears that the jellyfish-associated microbiome is discrete from the microbial community in the ambient seawater, exhibiting a certain degree of specialization with some preferences for specific jellyfish taxa and for specific jellyfish populations, life stages, and body parts. In addition, different sampling approaches and methodologies to study the phylogenetic diversity of the jellyfish-associated microbiome are described and discussed. Finally, some general conclusions are drawn from the existing literature and future research directions are highlighted on the jellyfish-associated microbiome.
Ključne besede: Cnidaria, Ctenophora, biodiversity, bioactive compounds, microbial communities, blue biotechnology
Objavljeno v DiRROS: 06.08.2024; Ogledov: 165; Prenosov: 72
.pdf Celotno besedilo (860,11 KB)
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Potato Virus Y infection hinders potato defence response and renders plants more vulnerable to Colorado potato beetle attack
Marko Petek, Ana Rotter, Polona Kogovšek, Špela Baebler, Axel Mithöfer, Kristina Gruden, 2014, izvirni znanstveni članek

Povzetek: In the field, plants are challenged by more than one biotic stressor at the same time. In this study, the molecular interactions between potato (Solanum tuberosum L.), Colorado potato beetle (Leptinotarsa decemlineata Say; CPB) and Potato virus YNTN (PVYNTN) were investigated through analyses of gene expression in the potato leaves and the gut of the CPB larvae, and of the release of potato volatile compounds. CPB larval growth was enhanced when reared on secondary PVYNTN-infected plants, which was linked to decreased accumulation of transcripts associated with the antinutritional properties of potato. In PVYNTN-infected plants, ethylene signalling pathway induction and induction of auxin response transcription factors were attenuated, while no differences were observed in jasmonic acid (JA) signalling pathway. Similarly to rearing on virus-infected plants, CPB larvae gained more weight when reared on plants silenced in JA receptor gene (coi1). Although herbivore-induced defence mechanism is regulated predominantly by JA, response in coi1-silenced plants only partially corresponded to the one observed in PVYNTN-infected plants, confirming the role of other plant hormones in modulating this response. The release of β-barbatene and benzyl alcohol was different in healthy and PVYNTN-infected plants before CPB larvae infestation, implicating the importance of PVYNTN infection in plant communication with its environment. This was reflected in gene expression profiles of neighbouring plants showing different degree of defence response. This study thus contributes to our understanding of plant responses in agro-ecosystems.
Ključne besede: insect midgut transcriptional response, gene expression, plant defence, volatile organic compounds, potato
Objavljeno v DiRROS: 02.08.2024; Ogledov: 121; Prenosov: 93
.pdf Celotno besedilo (959,76 KB)
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Bioactive compounds in the oils of the autochthonous slovenian olive varieties ‘Buga’, ‘Črnica’ and ‘Drobnica’
Vasilij Valenčič, Milena Bučar-Miklavčič, Maja Podgornik, 2024, izvirni znanstveni članek

Povzetek: The adaptation of autochthonous olive varieties to local soil and climatic conditions can lead to a unique chemical composition and characteristics of olive oils that may differ from the generally accepted quality standards set out in the International Olive Oil Council strategy documents and EU regulations. Therefore, the fatty acid composition, biophenol, tocopherol, sterol and triterpenic dialcohol content and composition of the autochthonous Slovenian olive varieties ‘Buga’, ‘Crnica’ ˇ and ‘Drobnica’ were studied for a three-year period with the aim of valorising the characteristics of the three olive varieties. Standardised and accredited analytical methods in accordance with SIST EN ISO/IEC 17025:2017 were applied. The results of the investigation showed that the highest average amount of oleic acid (75.75%) was found in the oils of the ‘Crnica’ variety, followed by the ‘Drobnica’ ˇ (72.06%) and the ‘Buga’ (68.73%). All three varieties are a good source of total biophenols (‘Buga’ 616 mg/kg, ‘Drobnica’ 569 mg/kg and ‘Crnica’ 427 mg/kg) and ˇ α-tocopherol (‘Buga’ 378 mg/kg, ‘Drobnica’ 279 mg/kg, and ‘Crnica’ 243 mg/kg). ‘Buga’ and ‘Drobnica’ are characterised by high ˇ amounts of total sterols, 2468 mg/kg and 2391 mg/kg, respectively, while ‘Crnica’ oils, in comparison, ˇ showed a lower average value of total sterols (1351 mg/kg). Due to their exceptional chemical composition, ‘Buga’, ‘Crnica’ and ‘Drobnica’ show great potential for the further cultivation and ˇ valorisation of traditional olive oil production in the region, thus contributing to the preservation of biodiversity and local traditions. The quality parameters of olive oil from the autochthonous Slovenian olive varieties ‘Buga’, ‘Crnica’ and ‘Drobnica’ also fulfil the limits for extra virgin olive oil ˇ according to the Commission Delegated Regulation (EU) 2022/2104, despite local climatic influences. However, accelerated growth due to climatic changes affecting early harvest can lead to them falling outside these limits, which was observed in particular for the ‘Buga’ variety in terms of the linoleic acid content. This study emphasises the importance of timing the harvest to achieve optimum maturity and meet EU quality standards, taking into account the genetic makeup of the varieties and their response to the current climatic conditions.
Ključne besede: olive, autochthonous Slovenian olive varieties, olive oil, bioactive compounds, chemistry, characterisation
Objavljeno v DiRROS: 24.07.2024; Ogledov: 108; Prenosov: 52
.pdf Celotno besedilo (254,21 KB)
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Control of organic contaminants in groundwater by passive sampling and multivariate statistical analysis
Anja Koroša, Nina Mali, 2022, izvirni znanstveni članek

Povzetek: Organic contaminants in groundwater are among the most challenging chemical compound contaminants today, particularly when it comes to understanding their occurrence, origin, and relations in groundwater, as well as the transport processes, fate, and environmental impacts involved. This paper presents the use of active carbon fibre (AFC) passive sampling and multivariate statistical processing of the results to predict the possible occurrence of organic compounds (OCs) in groundwater and to determine the origin of various anthropogenic activity. This study aims to deepen our knowledge on the control of OCs in groundwater by introducing a multi-analytical and multielemental holistic approach, using the Dravsko polje aquifer, the largest intergranular aquifer in Slovenia, as an example.
Ključne besede: compounds of concern, groundwater contaminants, organic compounds, passive sampling
Objavljeno v DiRROS: 22.06.2022; Ogledov: 794; Prenosov: 300
.pdf Celotno besedilo (11,61 MB)

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Emerging organic compounds in European groundwater
S. Y. Bunting, D. J. Lapworth, E. J. Crane, J. Grima-Olmedo, Anja Koroša, A. Kuczyńska, Nina Mali, L. Rosenqvist, M. E. van Vliet, A. Togola, B. Lopez, 2021, izvirni znanstveni članek

Povzetek: In Europe, emerging organic compounds (EOCs) in groundwater is a growing research area. Prioritisationfor monitoring EOCs in Europe was formalised in 2019 through the development of thefirst voluntarygroundwater watch list (GWWL). Despite this, groundwater occurrence data in the peer reviewedliterature for Europe has not been reviewed to date. Questions surrounding the effect, toxicity, move-ment in the subsurface and unsaturated zone make the process of regulating EOC use difficult. The aim inEurope is to develop a unified strategy for the classification, and prioritisation of EOCs to be monitored ingroundwater. This paper compiles evidence from the recent published studies from across Europe, since2012, when the last major literature global review of EOCs in groundwater took place. A total of 39studies were identified for review based on specific selection criteria (geography, publication date,sample size>10, inclusion of EOCs data). Data on specific compounds, and associated meta-data, arecompiled and reviewed. The two most frequently detected EOCs, carbamazepine and caffeine, occurredin groundwater at concentrations of up to 2.3 and 14.8mg/L, respectively.
Ključne besede: emerging organic chemicals, environmental exposure, groundwater contaminants, compounds of concern, groundwater hazards
Objavljeno v DiRROS: 13.01.2021; Ogledov: 1725; Prenosov: 767
.pdf Celotno besedilo (1,90 MB)

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