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Povzetek: Purpose Hyporheic biofilms are the central site for biogeochemical cycling in streams and rivers. In view of global warming and increasing human pressures, this study aimed to compare the response of hyporheic biofilm biomass and activities from an unpolluted reference stream reach surrounded by forest with those from a stream reach exposed to agricultural and urban land use using a mesocosm experiment in which the water temperature and dissolved organic carbon (DOC) contents were manipulated. Methods Hyporheic sediments collected in the field from the two study reaches (i.e. reference and impacted) were incubated in the laboratory at two different temperatures (10 °C, 14 °C) and wetted with three types of synthetic water (control [C] – 0 mg L−1; low DOC – 5 mg L−1; high DOC – 30 mg L−1) for four weeks. The responses of the hyporheic biofilms were measured weekly using structural (total protein content [TPC] as a proxy for biofilm biomass) and functional measures (electron transport system activity [ETSA] and community-level physiological profiling [CLPP]). Results The response of hyporheic biofilms to temperature changes and DOC enrichment was site-specific for all studied measures (TPC, ETSA and CLPP, including measured average well colour development [AWCD]). The addition of DOC to biofilms from the pristine stream reach significantly heightened the responses at 10 °C, a temperature within the normal environmental temperature ranges of the reference location, but not at 14 °C, which was here, a temperature outside normal environmental range. On the other hand, biofilms from the impacted stream reach exhibited increased responses following DOC enrichment under both temperature regimes, with a particularly pronounced response at 14 ºC, in this case, both experimental temperatures were within the normal environmental temperature ranges of the study locations. Conclusion Hyporheic biofilms were shown to be, like benthic biofilms, sensitive to temperature changes and organic enrichment, but their response to temperature changes and enrichment caused by climate change and/or other anthropogenic pressures (i.e. point and non-point pollution, removal of the riparian zone, hydromorphological modifications, etc.) was not simply linear but site-specific. The intensity of the response, characterized by increased activity and biomass production, appears to be constrained within the temperature ranges prevalent in the environment from which the biofilms originate. These findings emphasize the importance of site-specific considerations in predicting the impacts of climate change and anthropogenic pressures on these critical components of river and stream ecosystems.
Ključne besede: sediments, respiration, enzymes, functional indicators, interactions
Objavljeno v DiRROS: 05.08.2024; Ogledov: 298; Prenosov: 254
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Povzetek: The amount of plastic waste and microplastics released into marine environments has increased rapidly in recent decades. The durability of plastic materials results in major problems following their release into the environment. This study provides an overview of recent findings on issues related to plastic degradation, the accumulation of microplastics in mussels and fishes, and the toxicological effects associated with the ingestion of microplastics. These findings confirm the serious problem of slowly degrading plastics (which rarely degrade fully) in natural marine environments. Microplastics have become widespread pollutants and have been detected in mussels and fish around the world. Microplastic particles, whether virgin or with adsorbed pollutants on their surfaces, pose a health problem after being ingested by marine organisms. This paper ends by highlighting the need for certain improvements in studies of these phenomena.
Objavljeno v DiRROS: 05.08.2024; Ogledov: 301; Prenosov: 178
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Povzetek: Angiogenesis is required in cancer, including gynecological cancers, for the growth of primary tumors and secondary metastases. Development of anti-angiogenesis therapy in gynecological cancers and improvement of its efficacy have been a major focus of fundamental and clinical research. However, survival benefits of current anti-angiogenic agents, such as bevacizumab, in patients with gynecological cancer, are modest. Therefore, a better understanding of angiogenesis and the tumor microenvironment in gynecological cancers is urgently needed to develop more effective anti-angiogenic therapies, either or not in combination with other therapeutic approaches. We describe the molecular aspects of (tumor) blood vessel formation and the tumor microenvironment and provide an extensive clinical overview of current anti-angiogenic therapies for gynecological cancers. We discuss the different phenotypes of angiogenic endothelial cells as potential therapeutic targets, strategies aimed at intervention in their metabolism, and approaches targeting their (inflammatory) tumor microenvironment.
Ključne besede: angiogenesis, anti-angiogenic therapy, endothelial cells, endothelial cell metabolism, gynecological cancer, non-tip cells, tip cells, tumor microenvironment, vascular disrupting agents
Objavljeno v DiRROS: 05.08.2024; Ogledov: 257; Prenosov: 207
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Povzetek: Phytohormones are major signaling components that contribute to nearly all aspects of plant life. They constitute an interconnected communication network to fine-tune growth and development in response to the ever-changing environment. To this end, they have to coordinate with other signaling components, such as reactive oxygen species and calcium signals. On the one hand, the two endosymbiotic organelles, plastids and mitochondria, control various aspects of phytohormone signaling and harbor important steps of hormone precursor biosynthesis. On the other hand, phytohormones have feedback actions on organellar functions. In addition, organelles and phytohormones often act in parallel in a coordinated matter to regulate cellular functions. Therefore, linking organelle functions with increasing knowledge of phytohormone biosynthesis, perception, and signaling will reveal new aspects of plant stress tolerance. In this review, we highlight recent work on organelle–phytohormone interactions focusing on the major stress-related hormones abscisic acid, jasmonates, salicylic acid, and ethylene.
Ključne besede: abscisic acid (ABA), chloroplast, ethylene, mitochondria, jasmonates, plant organelles, phytohormones, salicylic acid (SA), plastids, stress signaling, retrograde signaling
Objavljeno v DiRROS: 05.08.2024; Ogledov: 269; Prenosov: 221
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Povzetek: The use of renewable plant biomass, lignocellulose, to produce biofuels and biochemicals using microbial cell factories plays a fundamental role in the future bioeconomy. The development of cell factories capable of efficiently fermenting complex biomass streams will improve the cost-effectiveness of microbial conversion processes. At present, inhibitory compounds found in hydrolysates of lignocellulosic biomass substantially influence the performance of a cell factory and the economic feasibility of lignocellulosic biofuels and chemicals. Here, we present and statistically analyze data on Saccharomyces cerevisiae mutants engineered for altered tolerance towards the most common inhibitors found in lignocellulosic hydrolysates: acetic acid, formic acid, furans, and phenolic compounds. We collected data from 7971 experiments including single overexpression or deletion of 3955 unique genes. The mutants included in the analysis had been shown to display increased or decreased tolerance to individual inhibitors or combinations of inhibitors found in lignocellulosic hydrolysates. Moreover, the data included mutants grown on synthetic hydrolysates, in which inhibitors were added at concentrations that mimicked those of lignocellulosic hydrolysates. Genetic engineering aimed at improving inhibitor or hydrolysate tolerance was shown to alter the specific growth rate or length of the lag phase, cell viability, and vitality, block fermentation, and decrease product yield. Different aspects of strain engineering aimed at improving hydrolysate tolerance, such as choice of strain and experimental set-up are discussed and put in relation to their biological relevance. While successful genetic engineering is often strain and condition dependent, we highlight the conserved role of regulators, transporters, and detoxifying enzymes in inhibitor tolerance. The compiled meta-analysis can guide future engineering attempts and aid the development of more efficient cell factories for the conversion of lignocellulosic biomass.
Objavljeno v DiRROS: 05.08.2024; Ogledov: 294; Prenosov: 260
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