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2. Digital PCR for the characterization of reference materialsMegan H. Cleveland, Hua-Jun He, Mojca Milavec, Young-Kyung Bae, Peter M. Vallone, Jim F. Huggett, 2024, pregledni znanstveni članek Povzetek: Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well as its role when developing and characterizing nucleic acid-based reference materials. Ključne besede: digital PCR, dPCR, reference materials, UV-spectroscopy Objavljeno v DiRROS: 03.06.2024; Ogledov: 195; Prenosov: 89 Povezava na datoteko |
3. Roles of the reference service life (RSL) of buildings and the RSL of building components in the environmental impacts of buildingsTajda Potrč Obrecht, Roman Kunič, Sabina Jordan, Andraž Legat, 2019, objavljeni znanstveni prispevek na konferenci Povzetek: The Life Cycle Assessment of a building is a complex analysis that also involves the use of the predicted Reference Service Life (RSL) of the building components and materials, as well as the predicted RSL of the whole building. The RSL values of individual materials and building components can be obtained from different sources and are not exactly comparable. In the present study, the influence of selected RLS values on an LCA assessment was evaluated. Three different RSL databases were used as the sources of the data to estimate the environmental impacts of selected building components (internal wooden door and external finishing coat). Two scenarios were presented. In the first scenario a building component can be reused in another building, while in the second scenario the reuse of the building component is not possible. The study showed that dependent on the selected RSL database, the results can differ by up to a factor of five. Therefore, it is very important to describe clearly the maintenance scenarios for a building in order to have a reliable comparison of the results of LCA assessments. Ključne besede: reference service life, life cycle assessment, refurbishment Objavljeno v DiRROS: 08.03.2024; Ogledov: 271; Prenosov: 183 Celotno besedilo (2,89 MB) Gradivo ima več datotek! Več... |
4. An LCA methodolody for assessing the environmental impacts of building components before and after refurbishmentTajda Potrč Obrecht, Sabina Jordan, Andraž Legat, Marcella Ruschi Mendes Saade, Alexander Passer, 2021, izvirni znanstveni članek Povzetek: Refurbishment is one of the most important measures for reducing the environmental impacts of the construction sector in the near future. According to the life cycle assessment (LCA) methodology for buildings, the environmental impacts of refurbishment measures should be assessed within the whole life cycle of the building and reflected in separate modules. However, in practice, refurbishment is often treated as the beginning of a new building life cycle. This leads to difficulties in correctly assessing the environmental impacts for the components that are reused or recycled after the refurbishment. The division of a building’s life cycle into two separate life cycles indicates that the environmental impacts must be divided between the life cycle before and the life cycle after the refurbishment for a correct assessment of the environmental impacts and a calculation of the residual value. We propose a newly developed methodology for calculating the environmental impacts and the residual value of refurbishment measures that also involves a division between life cycles. The new methodology is a combi-nation of already exiting methodologies that are innovatively combined and consists of four sequential steps. In the first step, the input, output and reuse flows between the life cycles before and after the refurbishment are defined. In the second step, the environmental impacts are assessed using the chosen allocation approach (i.e., the cut-off, cut-off with module D, avoided-burden, 50:50 and the product environmental footprint (PEF)). In the third step, a maintenance scenario is implemented according to the selected reference-service-life (RSL) database. In the fourth step, the residual value is estimated. The methodology was tested on selected building components. A sensitivity analysis for different allocation approaches and RSL databases was performed to show how the choice of these parameters can influence the results. The differences between the selected allocation approaches emerge if materials with recycled content are used or if the materials are being recycled or reused at the end of their life cycle. The developed methodology reliably estimates the environmental impacts as well as the residual value of the life cycle before and after the refurbishment. We expect that this research will stimulate practitioners to avoid the negligence of previous environmental flows, bringing scientific consistency to future assessments of refurbishment measures. Ključne besede: Life cycle assessment (LCA), refurbishment, allocation approaches, residual value, reference service life (RSL) Objavljeno v DiRROS: 28.07.2023; Ogledov: 470; Prenosov: 311 Celotno besedilo (8,59 MB) Gradivo ima več datotek! Več... |
5. Tree and stand growth differ among soil classes in semi-natural forests in central EuropeAndrej Bončina, Matija Klopčič, Vasilije Trifković, Andrej Ficko, Primož Simončič, 2023, izvirni znanstveni članek Povzetek: We determined the size of differences in stand and tree growth in semi-natural forests with respect to 16 reference soil groups. The forest area of Slovenia (11.8 thousand km2) was used as the study area, and reference soil units were derived from the national soil map at a 1:25,000 scale consisting of 10,781 polygons with an average size of 117.95 ha. Stand growth was defined as periodic stand basal area increment, while the growth of Norway spruce, silver fir, Scots pine, European beech and sessile oak trees was estimated by the periodic diameter increment of 238,349 dominant trees on 67,061 permanent sampling plots. A linear fixed-effects model and linear mixed-effect models were used for studying stand and tree growth in different site, stand and tree conditions. The soil unit was the dummy variable with Dystric Cambisols set as the reference category. Soil contributed 4.3 % to the explained variability of basal area increment and 4–27 % to the explained variability of the diameter increment of the five tree species. Soil was a stronger driver of stand and tree growth than climate or topography. Stand and tree species production rate on soil units was in the interval of −28 % to +5 % and −47 % to +14 % of that on the reference soil unit, respectively. Stand growth was the highest on Eutric Gleysols and the lowest on Histosols, and tree species generally exhibited the highest and the lowest growth rates on different soil units. We suggest that soil should be considered in growth models and studied interrelatedly with climatic, site and stand variables. Ključne besede: reference soil groups, FAO soil unit, natural forest, stand growth, tree growth Objavljeno v DiRROS: 29.12.2022; Ogledov: 685; Prenosov: 363 Celotno besedilo (2,23 MB) Gradivo ima več datotek! Več... |
6. Effect of temperature on MgO activity in the CaO-SiO[sub]2-MgO-Al[sub]2O[sub]3 system measured by molten tinYongchun Guo, Fengman Shen, Haiyan Zheng, Shuo Wang, Xin Jiang, Qiangjian Gao, 2022, izvirni znanstveni članek Ključne besede: blast furnace, slag system, activity of MgO, reference-slag, method, enthalpy, solution Objavljeno v DiRROS: 22.06.2022; Ogledov: 593; Prenosov: 180 Povezava na datoteko |
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8. Microarray-based uncovering of reference genes for quantitative real-time PCR in potato tuber infected with PVYBarbara Gerič Stare, Aleš Sedlar, Vladimir Meglič, 2021, izvirni znanstveni članek Ključne besede: potato, potato tuber, tuber infection, potato virus Y, real-time PCR, PCR, polymerase chain reaction, reference genes, stored ppotato tubers, pathogens Objavljeno v DiRROS: 31.05.2021; Ogledov: 1546; Prenosov: 341 Celotno besedilo (488,30 KB) |
9. The effect of harvesting on national forest carbon sinks up to 2050 simulated by the CBM-CFS3 model : a case study from SloveniaJernej Jevšenak, Matija Klopčič, Boštjan Mali, 2020, izvirni znanstveni članek Ključne besede: carbon, forest reference levels, forest management, harvesting scenarios, yield curves, UNFCCC, prirastne krivulje, UNFCCC Objavljeno v DiRROS: 19.10.2020; Ogledov: 1401; Prenosov: 962 Celotno besedilo (3,39 MB) Gradivo ima več datotek! Več... |
10. Definition, aims, and implementation of GA [sup] 2 LEN/HAEi Angioedema Centers of Reference and ExcellenceMarcus Maurer, Werner Aberer, Rosana Agondi, Mona Al-Ahmad, Maryam Ali Al-Nesf, Ignacio J. Ansotegui, Rand Arnaout, Luisa Karla Arruda, Riccardo Asero, Emel Aygören-Pürsü, Mojca Bizjak, Mitja Košnik, 2020, drugi znanstveni članki Ključne besede: centres of reference and excelence, Global Allergy and Asthma European Network, GA2LEN Objavljeno v DiRROS: 08.07.2020; Ogledov: 1880; Prenosov: 1046 Celotno besedilo (5,19 MB) Gradivo ima več datotek! Več... |