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445. The onset of Pliocene – Early Pleistocene fluvial aggradation in the Southeastern Alpine Foreland (Velenje Basin, Slovenia) and its paleoenvironmental implicationsEva Mencin Gale, Petra Jamšek Rupnik, Naki Akçar, Marcus Christl, Christof Vockenhuber, Flavio S. Anselmetti, Andrej Šmuc, 2024, izvirni znanstveni članek Povzetek: In this study, we focused on the Pliocene–Early Pleistocene fluvial terraces in the Velenje Basin andreconstructed the morphostratigraphy, sedimentary depositional environment, provenance and age of the graveldeposits using geomorphological, sedimentological, petrographic and chronological analyses. Geomorphologicalmapping revealed the presence of two main river‐terrace groups. The terraces in the older terrace group are severelydegraded and preserved only as remnants capping high ground, while in contrast the younger group is betterpreserved. Detailed lithofacies analyses of four selected stratigraphic sections of the older terrace group show that thegravel was deposited in a meandering and wandering environment. The gravel consists of metamorphic, igneous,volcaniclastic, clastic and carbonate lithologies derived from the north, east and west from the Paka Rivercatchments. To determine the timing of deposition, we performed isochron‐burial dating using cosmogenic 26Al and10 Be. Our new age constraints date the deposition of the older terrace group to 2.7 ± 0.3 Ma. Establishing theaggradation and incision model of the Velenje Basin documents pronounced regional tectonic uplift during thePliocene–Early Pleistocene, which led to incision and the subsequent formation of a terrace staircase.
Ključne besede: isochron‐burial dating, morphostratigraphy, provenance analysis, river terraces, Velenje Basin
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446. Poročilo o preskusu št.: LVG 2024-108 : vzorec št. 2024/00625Barbara Piškur, Patricija Podkrajšek, Špela Hočevar, 2024, izvedensko mnenje, arbitražna odločba Ključne besede: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, borov smolasti rak, PCR v realnem času
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447. Poročilo o preskusu št.: LVG 2024-107 : vzorec št. 2024/00635Barbara Piškur, Patricija Podkrajšek, Špela Hočevar, 2024, izvedensko mnenje, arbitražna odločba Ključne besede: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, borov smolasti rak, PCR v realnem času
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448. Poročilo o preskusu št.: LVG 2024-106 : vzorec št. 2024/00626Barbara Piškur, Patricija Podkrajšek, Špela Hočevar, 2024, izvedensko mnenje, arbitražna odločba Ključne besede: varstvo gozdov, morfološke analize, program preiskav, Geosmithia morbida, Juglans, PCR v realnem času
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449. Report on economic justification of the project : LIFE Stop CyanoBloomMaja Zupančič Justin, Gorazd Lakovič, Tinkara Rozina, Bojan Sedmak, Marko Gerl, 2017, končno poročilo o rezultatih raziskav Povzetek: The Annexe 17 of the Final Report of the project LIFE Stop CyanoBloom, presenting the “Report on economic justification of the project” is composed of 14 chapters. The first part of the report gives a general overview of the cyanobacteria and their blooms, and species and toxicity of cyanobacterial toxins. With a literature review, economic consequences of cyanobacterial blooms on human health, commercial fishery, tourism and recreation, and lake monitoring and management have been evaluated. The chapter on phytoplankton and bacterioplankton monitoring options presents the importance of the phytoplankton for the lake ecology and evaluation of the water quality status. Traditional phytoplankton monitoring approaches are compared to the new monitoring options applied in the project. The chapter in-lake cyanobacterial control options give a broad review of available in-lake rehabilitation and remediation options, which can be compared with the electrochemical oxidation technique presented in the following chapter and applied in the project. According to the results obtained during the pilot testing activities, a cost evaluation of the approach has been performed and presented in the report. The last chapter gathers open issues and proposals for the further work in this field.
Ključne besede: cyanobacteria, cyanotoxins, health risk
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450. Recommendation for the unit of measurement and the measuring system to report traceable and comparable results expressing GM content in accordance with EU legislationPhilippe Corbisier, Dejan Štebih, 2017, končno poročilo o rezultatih raziskav Povzetek: It is important to guarantee that results expressing the GM content are reliable, comparable and fulfil the requirements of existing EU legislation. The use of different measurement units to express a GM content, the appearance of new analytical methods that do not require a calibrant and the composite EU legislation on GMOs have triggered the need for a document to clarify how to obtain reliable and comparable results.
For this guidance document, past and current EU legislations have been reviewed with a special emphasis on what is meant by 'GM percentage' in the different legal texts. The metrological traceability of measurement results and the currently available guidance are explained and summarised. The particular case of botanical impurities and the genetic constitution of GM seeds are described and illustrated to better understand the complexity hidden behind this type of analysis. An overview of the different analytical methods based on DNA measurements and used for the expression of quantitative GM content results is provided, including the use of new techniques based on digital PCR (dPCR).
A measuring system that allows for comparing results by making them traceable to the same reference system has been elaborated in detail. Needs and tools are described and a solution has been proposed to convert results expressing GM content to the required measurement unit, whenever this is needed.
By following these recommendations, results obtained in GM copy number per haploid genome equivalent (cp/HGE) by dPCR can be converted into mass fraction percentage and compared to the results obtained by quantitative PCR (qPCR) either with a calibrant certified for its GM mass fraction or with a calibrant certified for its GM purity.
The general principle is to relate a measurement result to a GM quantity embedded in a specified certified reference material (CRM) either directly or via one single conversion factor (CF) per event. This conversion factor and its related uncertainty need to be determined precisely for each CRM batch, preferably on the pure GM CRM (100 %), using, for example, dPCR. The estimated uncertainty associated with this conversion factor must be integrated into the measurement uncertainty of the final results expressed in GM mass fraction.
CF are currently not yet established for most CRMs. CF values have been recently reported in a few pioneer dPCR studies. However, such proof of concept studies remain incomplete. Therefore, to avoid a gap between new technologies and current EU regulation, the working group recommends to launch a dedicated study to determine CF values on CRMs. Such a study should involve a limited number of competent laboratories with a proven experience in dPCR. The study could be coordinated by the EURL-GMFF.
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