1. Biocompatible polyelectrolyte multilayers with copper oxide and zinc oxide nanoparticles for inhibiting bacterial growthNives Matijaković Mlinarić, Stefanie Altenried, Atiđa Selmani, Juraj Nikolić, Aleksander Učakar, Anamarija Zore, Anže Abram, Sandro Lehner, Andrijana Sever Škapin, Monika Kušter, Eva Roblegg, Davor Kovačević, Qun Ren, Klemen Bohinc, 2024, izvirni znanstveni članek Povzetek: The prevalence of bacterial infections presents a significant challenge in the medical field, demanding effective strategies to impede bacterial adhesion and growth on various surfaces. The conducted study investigates the efficacy of polyelectrolyte multilayers─comprising poly(allylamine hydrochloride) (PAH) and alginate (ALG)─embedded with zinc oxide (ZnO) and copper oxide (CuO) nanoparticles (NPs) to inhibit bacterial adhesion on stainless-steel surfaces. Surface characterization involved zeta potential, contact angle, and roughness assessments. The effect of NP composition, size, and morphology in conjunction with polycation or polyanion terminating multilayers was evaluated against planktonic and surface-adhered Escherichia coli (E. coli) cells. Surfaces with the positively charged PAH-terminating multilayer displayed higher water contact angles (≈ 63°) than the negatively charged ALG-terminating multilayers (≈ 45°). Multilayers containing ZnO NPs showed a significant inhibition of planktonic E. coli growth, >99%. Moreover, complete growth inhibition of surface-adhered E. coli was achieved for multilayers containing both ZnO and CuO. Due to their larger specific surface area, rod-like ZnO NPs displayed higher antibacterial activity. The samples with ALG as the terminating layer showed more substantial antibacterial properties than samples with PAH as the terminating layer. Biocompatibility tests on immortalized human keratinocyte cells revealed good compatibility with multilayers incorporating NPs. In summary, this study underscores the potential of ZnO and CuO NPs within PAH/ALG multilayers for antibacterial applications without compromising their cytocompatibility.
Ključne besede: polyelectrolyte multilayers, alginate, poly(allylamine hydrochloride), CuO, ZnO, nanoparticles, Escherichia coli
Objavljeno v DiRROS: 06.06.2024; Ogledov: 69; Prenosov: 69
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2. Post-polymerization modification of poly(L-glutamic acid) with d-(+)-glucosaminePeter Perdih, Sašo Čebašek, Alenka Možir, Ema Žagar, 2014, izvirni znanstveni članek Povzetek: Carboxyl functional groups of poly(L-glutamic acid) (PGlu) were modified with
a D-(+)-glucosamine (GlcN) by amidation using 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-
methylmorpholinium chloride (DMTMM) as a coupling reagent. The coupling reaction
was performed in aqueous medium without protection of hydroxyl functional groups of
D-(+)-glucosamine. Poly(L-glutamic acid) and GlcN functionalized polyglutamates
(P(Glu-GlcN)) were thoroughly characterized by 1D and 2D NMR spectroscopy and
SEC-MALS to gain detailed information on their structure, composition and molar mass
characteristics. The results reveal successful functionalization with GlcN through the
amide bond and also to a minor extent through ester bond formation in position 1 of GlcN.
In addition, a ratio between the α- and β-form of glucosamine substituent coupled to
polyglutamate repeating units as well as the content of residual dimethoxy triazinyl active
ester moiety in the samples were evaluated.
Ključne besede: poly(L-glutamic acid), glucosamine, glycopolypeptide
Objavljeno v DiRROS: 16.12.2014; Ogledov: 4648; Prenosov: 933
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