1991. Characterization of hFOB 1.19 cell line for studying Zn-based degradable metallic biomaterialsEva Jablonská, Lucie Mrázková, Jiří Kubásek, Dalibor Vojtěch, Irena Paulin, Tomáš Ruml, Jan Lipov, 2024, izvirni znanstveni članek Povzetek: In vitro testing is the first important step in the development of new biomaterials. The human fetal osteoblast cell line hFOB 1.19 is a very promising cell model; however, there are vast discrepancies in cultivation protocols, especially in the cultivation temperature and the presence of the selection reagent, geneticin (G418). We intended to use hFOB 1.19 for the testing of Zn-based degradable metallic materials. However, the sensitivity of hFOB 1.19 to zinc ions has not yet been studied. Therefore, we compared the toxicity of zinc towards hFOB 1.19 under different conditions and compared it with that of the L929 mouse fibroblast cell line. We also tested the cytotoxicity of three types of Zn-based biomaterials in two types of media. The presence of G418 used as a selection reagent decreased the sensitivity of hFOB 1.19 to Zn2+. hFOB 1.19 cell line was more sensitive to Zn2+ at elevated (restrictive) temperatures. hFOB 1.19 cell line was less sensitive to Zn2+ than L929 cell line (both as ZnCl2 and extracts of alloys). Therefore, the appropriate cultivation conditions of hFOB 1.19 during biomaterial testing should be chosen with caution. Ključne besede: zinc degradable materials, in vitro cytotoxicity testing, hFOB 1.19 osteoblasts Objavljeno v DiRROS: 28.02.2024; Ogledov: 413; Prenosov: 156 Celotno besedilo (1,81 MB) Gradivo ima več datotek! Več... |
1992. |
1993. |
1994. |
1995. |
1996. Uporaba trajnostnih poliolefinov za brizgano zaporno embalažo : magistrsko deloRebeka Nenadović, 2023, magistrsko delo Ključne besede: trajnost, poliolefini, polipropilen, polietilen, brizganje, bio-osnovani poliolefini, reciklirani polimeri, biopolimeri, magistrsko delo Objavljeno v DiRROS: 27.02.2024; Ogledov: 503; Prenosov: 0 Celotno besedilo (3,82 MB) |
1997. |
1998. Vpliv temperature in gostote na mehanske lastnosti ekspandiranega polistirena : magistrsko deloJaka Vaupot, 2023, magistrsko delo Ključne besede: ekspandiran polistiren, tlačna trdnost, natezna trdnost, temperaturno utrujanje materiala, povišana temperatura, mikrostruktura, dimenzijska stabilnost, magistrsko delo Objavljeno v DiRROS: 27.02.2024; Ogledov: 472; Prenosov: 158 Celotno besedilo (2,34 MB) |
1999. |
2000. |