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2. Electroporator for in vitro cell permeabilizationMarko Puc, Karel Flisar, Stanislav Reberšek, Damijan Miklavčič, 2001, izvirni znanstveni članek Povzetek: The use of high voltage electric pulse technology, electroporation, in cell biology, biotechnology and medicine has attracted an enormous interest. Electroporation is a transient phenomenon that increases the permeability of cell plasma membrane. In the state of high permeability, the plasma membrane allows small and large molecules to be introduced into the cytoplasm, althoughthe cell plasma membrane represents a considerable barrier for them inits normal state. The effectiveness of electroporation depends on many parameters that can be divided into the parameters of the electric field and the parameters that define the state of cells and their surrounding i.e, temperature, osmotic pressure, etc. In this article, we present a prototype electroporator GT-1 for in vitro electropermeabilization that we have developed. Our electroporator offers a vast flexibility of parameters and can generate high and low voltage pulses, of which the latter ones are used for electrophoretic transfer of charged molecules through permeabilized cell plasma membrane.
Ključne besede: electroporation instrumentation methods, cell membrane permeabilization
Objavljeno v DiRROS: 25.01.2024; Ogledov: 350; Prenosov: 97
 Celotno besedilo (239,19 KB) |
3. On mechanisms of cell plasma membrane vesiculationVeronika Kralj-Iglič, Urška Batista, Henry Hägerstrand, Aleš Iglič, Janja Majhenc, Mihael Sok, 1998, izvirni znanstveni članek Ključne besede: budding, membrane bilayer, vesiculation
Objavljeno v DiRROS: 19.01.2024; Ogledov: 245; Prenosov: 79
Celotno besedilo (1,68 MB) |
4. Improved therapeutic effect of electrochemotherapy with cisplatin by intratumoral drug administration and changing of electrode orientation for electropermeabilization on EAT tumor model in miceMaja Čemažar, Damijan Miklavčič, Lojze Vodovnik, Tomaž Jarm, Zvonimir Rudolf, Borut Štabuc, Tanja Čufer, Gregor Serša, 1995, izvirni znanstveni članek Ključne besede: neoplasmas, experimental therapy, cisplatin, electric stimulation therapy, cell membrane permeability
Objavljeno v DiRROS: 15.01.2024; Ogledov: 261; Prenosov: 68
Celotno besedilo (393,12 KB) |
5. Mechanistic study of fast performance decay of PtCu alloy-based catalyst layers for polymer electrolyte fuel cells through electrochemical impedance spectroscopyMaximilian Grandi, Matija Gatalo, Ana Rebeka Kamšek, Gregor Kapun, Kurt Mayer, Francisco Ruiz-Zepeda, Martin Šala, Bernhard Marius, Marjan Bele, Nejc Hodnik, Merit Bodner, Miran Gaberšček, Viktor Hacker, 2023, izvirni znanstveni članek Ključne besede: PEFC, catalyst layer, platinum–copper, degradation, ionomer, electrochemical impedance spectroscopy, membrane electrode assembly
Objavljeno v DiRROS: 23.06.2023; Ogledov: 500; Prenosov: 229
Celotno besedilo (2,26 MB)
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7. Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathonZala Jan, Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drašler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič, 2021, izvirni znanstveni članek Povzetek: Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.
Ključne besede: membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon
Objavljeno v DiRROS: 25.01.2021; Ogledov: 1435; Prenosov: 4454
Celotno besedilo (1,88 MB)
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8. Design of protein logic gate system operating on lipid membranesNeža Omersa, Saša Rezelj, Matic Kisovec, Marjetka Podobnik, Gregor Anderluh, 2020, izvirni znanstveni članek Ključne besede: pore-forming toxin, listeriolysin O, protein logic gates, lipid membrane, DARPin, pH
Objavljeno v DiRROS: 25.11.2020; Ogledov: 1664; Prenosov: 921
Celotno besedilo (4,48 MB)
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9. Comparison of Pt-Cu/C with benchmark Pt-Co/C : metal dissolution and their surface interactionsMatija Gatalo, Primož Jovanovič, Urša Petek, Martin Šala, Vid Simon Šelih, Francisco Ruiz-Zepeda, Marjan Bele, Nejc Hodnik, Miran Gaberšček, 2019, izvirni znanstveni članek Ključne besede: oxygen reduction reaction, proton exchange membrane fuel cell, platinum alloy, metal dissolution, electrochemical flow cell coupled to inductively coupled plasma mass spectrometer
Objavljeno v DiRROS: 23.11.2020; Ogledov: 1603; Prenosov: 1148
Celotno besedilo (4,12 MB)
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