1951. Morphological and molecular characterization of Micromeria croatica (Lamiaceae), an endemic and potentially valuable horticultural species of the Dinaric AlpsZlatko Liber, Ivan Radosavljević, Zlatko Šatović, Marija Hodja, Vesna Židovec, Faruk Bogunić, Dalibor Ballian, Danijela Stešević, Sven D. Jelaska, Dario Kremer, 2023, izvirni znanstveni članek Povzetek: Due to climate extremes and limited natural resources, especially water, we can expect increased demand in the future for species that can better tolerate climate extremes such as drought. One potentially valuable horticultural species is the endemic species of the Dinaride Mountains Micromeria croatica (Pers.) Schott (family Lamiaceae). It grows in the crevices of carbonate rocks, extending from an altitude of 150 m to more than 2000 m. This study aims to provide additional insight into the genetic and morphological diversity of this endemic species, focusing on valuable horticultural traits. To achieve this goal, morphological and molecular analyses were performed on ten natural populations. Through STRUCTURE and PCoA analyses, ten M. croatica populations were placed into western and eastern genetic groups, with several individuals from western populations assigned to the eastern group and vice versa. These atypical individuals assigned to the new genetic group by BAPS analysis indicate gene flow between western and eastern populations. Similarly, an analysis of molecular variance revealed fewer genetic differences than within studied populations. Both PCA and CANDISC analysis based on eleven morphological traits largely confirmed the existence of two slightly different genetic groups. Two populations containing plants with the most flowers per shoot, one with white-flowered individuals, one with the roundest leaves, and one with the narrowest leaves proved to be the most horticulturally valuable. The genetic and morphological variability found should be a sufficient basis for the potential selection of M. croatica populations and individuals for horticultural purposes. Ključne besede: morphological characterization, molecular characterization, Micromeria croatica (Lamiaceae), Dinaric Alps Objavljeno v DiRROS: 19.03.2024; Ogledov: 406; Prenosov: 219 Celotno besedilo (3,55 MB) Gradivo ima več datotek! Več... |
1952. Varijabilnost morfoloških svojstava listova europske crne topole i hibridnih crnih topola u klonskom arhivu u ŽepčuMirzeta Memišević Hodžić, Dalibor Ballian, 2023, izvirni znanstveni članek Povzetek: Black poplar (Populus nigra L.) is one of the most important European tree species, inhabiting alluvial habitats along the banks of large rivers. Today, it is one of the most endangered species of forest trees due to habitat devastation, regulation of river flows, excessive exploitation, and the introduction of non-native tree species with which it hybridized. This study aims to determine the variability of morphological properties of black poplar leaves n Bosnia and Herzegovina, within and among populations, and among river basins. Material from the clonal archive of black poplars was researched. The archive was founded in 2005 in Žepče. It contains clones from 161 trees of indigenous black poplars from 26 populations throughout Bosnia and Herzegovina (from the basins of 6 rivers) and 15 hybrid poplar species (table 1 and table 2). Ten leaves were collected from each clone by taking the fifth leaf from the top of the main shoot. Five leaves from each clone were measured. The measurement was performed with a digital movable scale with an accuracy of 0.1 mm and a protractor. The following traits were measured: leaf blade length, leaf blade width, petiole length, total leaf length, the angle between the first lateral nerve and central nerve, and distance from the leaf base to the widest part of the leaf. The number of teeth on one cm above the widest part of the leaf was counted. Data were processed using SPSS 26.0 and EXCEL by populations and river basins. Analysis of variance revealed that there are statistically significant differences among the studied populations (table 4), as well as among river basins (table 7), for all investigated traits. The petiole length trait showed the highest variability, and the lowest variability had the insertion angle of the first lateral and central nerve (table 3). The clones of population of hairy black poplars Čapljina had the lowest values of leaf traits and differed significantly in all measured properties from the others. The most common number of teeth per 1 cm of leaf edge for all populations was four teeth (figure 2), which appeared on 38.3% of leaves. This research gives us a small insight into the morphological characteristics of black poplars in the clone archive Žepče and can be the basis for further researches of the traits of black poplars needed for a successful continuation of work on breeding this species Ključne besede: european black poplar, clone archive, morphological traits of laeves Objavljeno v DiRROS: 19.03.2024; Ogledov: 480; Prenosov: 204 Celotno besedilo (991,66 KB) Gradivo ima več datotek! Več... |
1953. Clustering of field maple populations from different ecological conditions in Bosnia and Herzegovina based on discriminant analysis of morphological traitsStjepan Kvesić, Dalibor Ballian, Mirzeta Memišević Hodžić, 2023, izvirni znanstveni članek Povzetek: Acer campestre L. is naturally distributed in most of Europe. The species can serve as a valuable model for researching the sensitivity of populations to habitat fragmentation. This research aims to determine whether there is a differentiation of field maple populations based on their division by ecological-vegetation regions, precipitation amounts, temperatures, and altitudes of the populations. The material was collected from 25 populations in Bosnia and Herzegovina, from different ecological-vegetation areas, with twelve trees per population and five fruits and ten leaves per tree. Then, 10 fruit properties and 19 leaf properties were analyzed. A discriminant analysis was performed for population groups based on the ecological-vegetation area, amount of precipitation, average annual air temperature and altitude. The discriminant analysis results showed the distinguishing of groups of populations in the sub-Mediterranean area, and groups of populations with an average annual temperature of 14.00 to 15.99 °C. There was no clear separation of the groups according to the average annual precipitation or altitude. The analysis of the connection established that the influence of environmental factors is more pronounced compared to geographical factors. The key ecological variable that determines morphological separation was temperature and to a lesser extent precipitation. The results of this research will be used in planning of afforestation of suitable, unvegetated land and extreme habitats in the southern and southwestern parts of Bosnia and Herzegovina with Acer campestre. Ključne besede: Acer campestre, discriminant analysis, ecological-vegetation reionization, population groups differentiation Objavljeno v DiRROS: 19.03.2024; Ogledov: 411; Prenosov: 252 Celotno besedilo (1,11 MB) Gradivo ima več datotek! Več... |
1954. Cathepsin H indirectly regulates morphogenetic protein-4 (BMP-4) in various human cell linesMatija Rojnik, Zala Jevnikar, Bojana Mirković, Damjan Janeš, Nace Zidar, Danijel Kikelj, Janko Kos, 2011, izvirni znanstveni članek Povzetek: Background. Cathepsin H is a cysteine protease considered to play a major role in tumor progression, however, its precise function in tumorigenesis is unclear. Cathepsin H was recently proposed to be involved in processing of bone morphogenetic protein 4 (BMP-4) in mice. In order to clarify whether cathepsin H also regulates BMP-4 in humans, its impact on BMP-4 expression, processing and degradation was investigated in prostate cancer (PC-3), osteosarcoma (HOS) and pro-monocytic (U937) human cell lines. Materials and methods. BMP-4 expression was founded to be regulated by cathepsin H using PCR array technology and confirmed by real time PCR. Immunoassays including Western blot and confocal microscopy were used to evaluate the influence of cathepsin H on BMP-4 processing. Results. In contrast to HOS, the expression of BMP-4 mRNA in U937 and PC3 cells was significantly decreased by cathepsin H. The different regulation of BMP-4 synthesis could be associated with the absence of the mature 28 kDa cathepsin H form in HOS cells, where only the intermediate 30 kDa form was observed. No co-localization of BMP-4 and cathepsin H was observed in human cell lines and the multistep processing of BMP-4 was not altered in the presence of specific cathepsin H inhibitor. Isolated cathepsin H does not cleave mature recombinant BMP-4, neither with its amino- nor its endopeptidase activity. Conclusions. Our results exclude direct proteolytic processing of BMP-4 by cathepsin H, however, they provide support for its involvement in the regulation of BMP-4 expression. Objavljeno v DiRROS: 18.03.2024; Ogledov: 575; Prenosov: 423 Celotno besedilo (722,81 KB) Gradivo ima več datotek! Več... |
1955. Cathepsin H indirectly regulates morphogenetic protein-4 (BMP-4) in various human cell linesMatija Rojnik, Zala Jevnikar, Bojana Mirković, Damjan Janeš, Nace Zidar, Danijel Kikelj, Janko Kos, 2011, izvirni znanstveni članek Povzetek: Background. Cathepsin H is a cysteine protease considered to play a major role in tumor progression, however, its precise function in tumorigenesis is unclear. Cathepsin H was recently proposed to be involved in processing of bone morphogenetic protein 4 (BMP-4) in mice. In order to clarify whether cathepsin H also regulates BMP-4 in humans, its impact on BMP-4 expression, processing and degradation was investigated in prostate cancer (PC-3), osteosarcoma (HOS) and pro-monocytic (U937) human cell lines. Materials and methods. BMP-4 expression was founded to be regulated by cathepsin H using PCR array technology and confirmed by real time PCR. Immunoassays including Western blot and confocal microscopy were used to evaluate the influence of cathepsin H on BMP-4 processing. Results. In contrast to HOS, the expression of BMP-4 mRNA in U937 and PC3 cells was significantly decreased by cathepsin H. The different regulation of BMP-4 synthesis could be associated with the absence of the mature 28 kDa cathepsin H form in HOS cells, where only the intermediate 30 kDa form was observed. No co-localization of BMP-4 and cathepsin H was observed in human cell lines and the multistep processing of BMP-4 was not altered in the presence of specific cathepsin H inhibitor. Isolated cathepsin H does not cleave mature recombinant BMP-4, neither with its amino- nor its endopeptidase activity. Conclusions. Our results exclude direct proteolytic processing of BMP-4 by cathepsin H, however, they provide support for its involvement in the regulation of BMP-4 expression. Objavljeno v DiRROS: 18.03.2024; Ogledov: 448; Prenosov: 104 Povezava na datoteko |
1956. Seismogenic depth and seismic coupling estimation in the transition zone between Alps, Dinarides and Pannonian Basin for the new Slovenian seismic hazard modelPolona Zupančič, Barbara Šket Motnikar, Michele M. C. Carafa, Petra Jamšek Rupnik, Mladen Živčić, Vanja Kastelic, Gregor Rajh, Martina Čarman, Jure Atanackov, Andrej Gosar, 2024, izvirni znanstveni članek Povzetek: Seismogenic depth and seismic coupling are important inputs into seismic hazard estimates. Although the importance of seismic coupling is often overlooked, it significantly impacts seismic hazard results. We present an estimation of upper and lower seismogenic depth and expected hypocentral depth and seismic coupling in the transition zone between the Alps, Dinarides and Pannonian Basin, characterized by a complex deformation pattern, highly variable crustal thickness, and moderate seismic hazard, supporting the development of the 2021 seismic hazard model of Slovenia. The hazard model was based on three seismic source models: area source model, fault source model and smoothed seismicity (point) source model. We estimated the lower seismogenic depth using seismological and geological data and compared them. The seismological estimate was based on two regional earthquake catalogues prepared for this study. In the area source model, estimates of lower seismogenic depth from seismological data are deeper or equal to the ones derived from geological data, except in one case. In the fault source model, we analysed each fault individually and chose seismological lower depth estimates in 12 among 89 faults as more representative. The seismogenic thickness for each individual fault source was determined for seismic coupling determination. The seismic coupling was assessed by two approaches, i.e. we chose the most trusted value from the literature, and the value determined for each fault individually by using the approach based on the updated regional fault and earthquake data sets. The final estimate of seismic coupling ranges from 0.77 to 0.38. We compared the tectonic moment rate based on long-term slip rate using different values of seismic coupling with the seismic moment rate obtained from the earthquake catalogue. The analysis is done for the whole area, as well as for the individual area zones. The analysis of N–S components of estimated slip for the largest faults in the area of west Slovenia shows that the regional geological and geodetic shortening rates are comparable. The total activity rate of three global seismic source models is compared, which gives up to a 10 % difference. Our results contribute to a better understanding of the seismic activity in the region. The presented approach for seismic coupling estimation can be applied in cases where the total slip rate is given instead of its seismic part and can be used at regional or national level. The approach is also suitable for the cross-border harmonization of the European seismic hazard modelling data. Ključne besede: seismic hazard, modeling, Slovenia Objavljeno v DiRROS: 18.03.2024; Ogledov: 721; Prenosov: 509 Celotno besedilo (11,29 MB) Gradivo ima več datotek! Več... |
1957. Efficacy of first-line systemic treatment in correlation with BRAF V600E and different KRAS mutations in metastatic colorectal cancer : a single institution retrospective analysisMartina Reberšek, Marko Boc, Petra Škerl, Jernej Benedik, Zvezdana Hlebanja, Neva Volk, Srdjan Novaković, Janja Ocvirk, 2011, izvirni znanstveni članek Ključne besede: rak (medicina), debelo črevo, danka, zdravljenje Objavljeno v DiRROS: 18.03.2024; Ogledov: 439; Prenosov: 143 Celotno besedilo (461,04 KB) |
1958. Genotyping of BRCA1, BRCA2, p53, CDKN2A, MLH1 and MSH2 genes in a male patient with secondary breast cancerAna Lina Vodušek, Srdjan Novaković, Vida Stegel, Berta Jereb, 2011, izvirni znanstveni članek Objavljeno v DiRROS: 18.03.2024; Ogledov: 399; Prenosov: 108 Celotno besedilo (538,94 KB) |
1959. Sinonasal inverted papilloma associated with squamous cell carcinomaJasna But-Hadžić, Klemen Jenko, Mario Poljak, Boštjan Kocjan, Nina Gale, Primož Strojan, 2011, izvirni znanstveni članek Ključne besede: rak (medicina), papiloma virusi, nosna votlina, karcinom, zdravljenje, radioterapija Objavljeno v DiRROS: 18.03.2024; Ogledov: 493; Prenosov: 137 Celotno besedilo (513,64 KB) |
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