1. Histology basics and cell death detection in honeybee tissueMaja Ivana Smodiš Škerl, izvirni znanstveni članek Povzetek: Honeybees (Apis mellifera L.) inside the hive (nurse workers and other hive bees)
and outside the hive (foragers) are exposed to climate and weather changes, various
pesticides, pathogens, and malnutrition, mainly entering through the mouth and
primarily affecting the digestive tracts of adult bees. To understand and prevent the
effects of such external and internal stressors on honeybees, one useful research
method is the immunohistochemical method. A basic protocol is described to prepare
the midgut (ventriculus) and hypopharyngeal glands (HPGs) of adult bees for
histological analysis. A detailed methodology is described to assess the level of cell
damage and distinguish necrosis from programmed cell death (apoptosis) as a natural
process of tissue regeneration. The results of adult honeybee treatment with oxalic
acid and pesticides (insecticide and acaricide) and the determination of cell death in
the ventriculus and HPGs are presented. The pros and cons of the methodology are
also discussed. Ključne besede: cell death, honeybee, histology, environment Objavljeno v DiRROS: 08.07.2022; Ogledov: 710; Prenosov: 411 Celotno besedilo (1,31 MB) Gradivo ima več datotek! Več... |
2. Automated analysis of proliferating cells spatial organisation predicts prognosis in lung neuroendocrine neoplasmsMatteo Bulloni, Giada Sandrini, Irene Stacchiotti, M. Barberis, Fiorella Calabrese, Lina Carvalho, Gabriella Fontanini, Greta Alì, Francesco Fortarezza, Paul Hofman, Izidor Kern, 2021, izvirni znanstveni članek Ključne besede: neuroendocrine tumors, lung neoplasms, carcinoma, prognosis, pathology, histology, Ki-67 antigen, machine learning, image enhancement, lung cancer, neuroendocrine neoplasms Objavljeno v DiRROS: 09.11.2021; Ogledov: 997; Prenosov: 426 Povezava na datoteko |
3. Prognostic impact of PD-1 and PD-L1 expression in malignant pleural mesothelioma : an international multicenter studyLuka Brčić, Thomas Klikovits, Zsolt Megyesfalvi, Berta Mosleh, Katharina Sinn, Richard Hritcu, Viktoria Laszlo, Tanja Čufer, Aleš Rozman, Izidor Kern, Katja Mohorčič, 2021, izvirni znanstveni članek Povzetek: Background: Programmed cell death 1/programmed death ligand 1 (PD-1/PD-L1) immune-checkpoint blockade is a promising new therapeutic strategy in cancer. However, expression patterns and prognostic significance of PD-L1 and PD-1 are still controversial in human malignant pleural mesothelioma (MPM). Methods: Formalin-fixed paraffin-embedded (FFPE) tumor samples from 203 MPM patients receiving standard treatment without immunotherapy were collected from 5 European centers. PD-L1 and PD-1 expression of tumor cells (TCs) and tumor-infiltrating lymphocytes (TILs) were measured by immunohistochemistry and correlated with clinical parameters and long-term outcome. Results: High (>10%) PD-L1 TC and PD-1 TILs expressions were found in 18 (8%) and 39 (24%) patients, respectively. PD-L1 was rarely expressed by TILs [>/=1%, n=13 (8%); >10%, n=1]. No significant associations were found between the PD-L1 or PD-1 expression of TCs or TILs and clinicopathological parameters such as stage or histological subtype. Notably, patients with high (>10%) TC-specific PD-L1 expression exhibited significantly worse median overall survival (OS) (6.3 vs. 15.1 months of those with low TC PD-L1 expression; HR: 2.51, P<0.001). In multivariate cox regression analysis adjusted for clinical parameters, high TC PD-L1 expression (>10%) proved to be an independent negative prognostic factor for OS (HR: 2.486, P=0.005). There was no significant correlation between PD-L1 or PD-1 expression of TILs and OS. Conclusions: In this multicenter cohort study, we demonstrate that high (>10%) PD-L1 expression of TCs independently predicts worse OS in MPM. Further studies are warranted to investigate the value of PD-L1/PD-1 expression as a marker for treatment response in MPM patients receiving immunotherapy. Ključne besede: mesothelioma - anatomy and histology - analysis, 1malignant pleural mesothelioma, programmed death-ligand 1, programmed cell death 1, PD-L1 Objavljeno v DiRROS: 31.03.2021; Ogledov: 1469; Prenosov: 639 Povezava na datoteko |