1261. Flow cytometric pitfalls in immunophenotyping of lymphomasAlojz Ihan, 1998, pregledni znanstveni članek Povzetek: Flow cytometry has been introduced as the most reliable method for the analysis of lymphocyte subsets. The advantage of flow cytometry is that it enables application of virtually all monoclonal antibodies (native cells), a quantitative estimation of different lymphoid cells, and that cell can be simultaneously analysed for multiple marker expression. The usage of flow cytometers requiers considerable knowledge of physics and its technical application. Moreover, several problems arise from the complexity of the biolocal systems investigated.
Objavljeno v DiRROS: 19.01.2024; Ogledov: 267; Prenosov: 82
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1263. Efficacy of trapping protocols for Agrilus jewel beetles : a multi-country assessmentGiacomo Santoiemma, David Williams, Everett G. Booth, Giacomo Cavaletto, James Connell, Gianfranco Curletti, Maarten De Groot, Sarah M. Devine, Abigail Enston, Joseph A. Francese, Eva Groznik, Tine Hauptman, 2024, izvirni znanstveni članek Povzetek: The genus Agrilus is one of the most diverse insect genera worldwide. The larval feeding activity causes extensive damage in both forests and orchards. In addition, more than 30 species have been introduced outside their native range so far, including the emerald ash borer Agrilus planipennis Fairmaire. Thus, the availability of efficient trapping protocols for early detection of Agrilus species at entry points is of utmost importance. In this study we tested whether trapping protocols developed for surveillance of A. planipennis in North America were also effective for other Agrilus species. In particular, through a multi-country assessment we compared the efficacy of detecting Agrilus species on: (i) green glue-coated prism traps vs. green Fluon-coated multi-funnel traps when baited with the green leaf volatile (Z)-3-hexenol or left unbaited; and (ii) green multi-panel traps vs. green multi-panel traps baited with dead adult Agrilus beetles (decoys). A total of 23,481 individuals from 45 Agrilus species were caught. Trap design significantly affected both species richness and abundance of Agrilus species in several of the countries where the trapping experiments were carried out, and green prism traps outperformed green multi-funnel traps in most cases. On the contrary, the addition of a (Z)-3-hexenol lure or dead adult beetle decoys on to traps did not improve trap catches. Our study highlights that reliable trap models to survey Agrilus species are already available, but also that there is the clear need to further investigate chemical ecology of Agrilus species to develop semiochemical lures that can improve detection efficacy.
Ključne besede: biosecurity, early detection, forest pests, lures, traps, visual stimuli, Agrilus, beetles
Objavljeno v DiRROS: 19.01.2024; Ogledov: 365; Prenosov: 265
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Gradivo ima več datotek! Več... |
1264. Genetic polymorphisms of xenobiotic metabolizing enzymes in human colorectal cancerVita Dolžan, Metka Ravnik-Glavač, Katja Breskvar, 1998, objavljeni znanstveni prispevek na konferenci Povzetek: It was proposed that both hereaditary and environmental factors contribute to the development of colorectal cancer (CRC). Carcinogenic polycyclic aromatic hydrocarbons (PAHs) from food or tobacco smoke can form DNA adducts and thus initiate carcinogenesis after metabolic activation via cytochrome P4501A1 (CYP1A1). Intermediate metabolites are detoxified by conjugation with glutathione S-transferases. Our aim was to look for inherited metabolic suceptibility to CRC. We used PCR-based genotyping approach to determine the frequencies of polymorphic alleles of two cytochromes P450 (CYP2D6 and CYP1A1)and two glutathione S-transferases (GSTM1 and GSTT1) in DNA samples from 31 sporadic, 25 familial CRC cases and 73 healthy controls. The difference in frequencies of poor metabolisers due to CYP2D6 gene polymorphismwas close to the limit of statistical significance between sporadiCRC and healthy control group (lambda 2=5.52, m=2, p=0.06) despite the small sample size. The frequencies of either CYP1A1 MspI, GST M1 or GST T1 genotypes were not significantly different in both CRC cases and in controls. Although our study suggests some difference in metabolic susceptibility between sporadic and familial CRC, further studies are needed to investigate the combined effect of polymorphic genes involved in carcinogen metabolism in a larger group of patients with defined exposure to dietary carcinogens and smoking.
Objavljeno v DiRROS: 19.01.2024; Ogledov: 324; Prenosov: 82
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1267. On mechanisms of cell plasma membrane vesiculationVeronika Kralj-Iglič, Urška Batista, Henry Hägerstrand, Aleš Iglič, Janja Majhenc, Mihael Sok, 1998, izvirni znanstveni članek Ključne besede: budding, membrane bilayer, vesiculation
Objavljeno v DiRROS: 19.01.2024; Ogledov: 242; Prenosov: 79
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1270. Vinblastine increases antitumor effectiveness of bleomycinMaja Čemažar, Marija Auersperg, Gregor Serša, 1997, izvirni znanstveni članek Povzetek: In our previous vinblastine (VELBE) was shown to increase the plasma membrane fluidity. This effect of VELBE might be expolited for better transport of drugs through the plasma membrane. Bleomycin (BLM) is a highly cytotoxic drug when present inside the cells but has a hampered transport through the plasma membrane. The aim of the present study was to determine whether pretreatment with VELBE can increase the effect of BLM on intraperitoneal SA-1 tumors in mice. BLM and VELBE were used as single agents or in various combinations, i.e. VELBE and BLM injected simultanously, BLM injected 24 h before VELBE or VELBE injected 24 h before BLM. Mice survival was the end-point used for determining the effect of this combined treatments. VELBE and BLM as a single treatment significantly prolonged median survival time of study animals compared to controls. Furthermore, when VELBE and BLM were combined, all threetested combinations were more effective than VELBE or BLM as single treatments. The effect on animal survival was equal when VELBE was given 24 h after or simultanously with BLM. The longest survival, however was obtained when VELBE was injected 24 h before BLM. From these results we can conclude that the underlying mechanisms for more than additive effect of VELBE and BLM when VELBE was given 24 h before BLM could be attributed to an increased membrane fluidity, possibly in combination with a cell kinetic effect.
Objavljeno v DiRROS: 18.01.2024; Ogledov: 235; Prenosov: 60
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