31. Predlog optimalnega števila in lokacij kontrolno-lovnih nastav za prezimele osebke osmerozobegasmrekovega lubadarja ( Ips typographus ) v Slovenijiza leto 2025Nikica Ogris, Marija Kolšek, 2025, drugi znanstveni članki Povzetek: Pripravili smo predlog optimalnega števila in lokacij kontrolno-lovnih nastav za prezimele osebke osmerozobega smrekovega lubadarja (Ips typographus ) (nastave I. serije) za leto 2025. Pri pripravi smo sledili predlogu postopka za določitev optimalnega števila in lokacij kontrolno-lovnih nastav za osmerozobega smrekovega lubadarja. Predlagamo, da se nastave I. serije v letu 2025 položi na88 lokacijah. Ključne besede: gozdovi, varstvo gozdov, kontrolno-lovne nastave, postopek optimizacije, osmerozobi smrekov lubadar, Ips typographus, smreka, Picea abies, spremljanje, monitoring, zatiranje Objavljeno v DiRROS: 17.01.2025; Ogledov: 60; Prenosov: 35 Celotno besedilo (565,37 KB) Gradivo ima več datotek! Več... |
32. New treatment approaches for Clostridioides difficile infections : alternatives to antibiotics and fecal microbiota transplantationTomaž Bratkovič, Abida Zahirović, Maruša Bizjak, Maja Rupnik, Borut Štrukelj, Aleš Berlec, 2024, pregledni znanstveni članek Povzetek: Clostridioides difficile causes a range of debilitating intestinal symptoms that may be fatal. It is particularly problematic as a hospital-acquired infection, causing significant costs to the health care system. Antibiotics, such as vancomycin and fidaxomicin, are still the drugs of choice for C. difficile infections, but their effectiveness is limited, and microbial interventions are emerging as a new treatment option. This paper focuses on alternative treatment approaches, which are currently in various stages of development and can be divided into four therapeutic strategies. Direct killing of C. difficile (i) includes beside established antibiotics, less studied bacteriophages, and their derivatives, such as endolysins and tailocins. Restoration of microbiota composition and function (ii) is achieved with fecal microbiota transplantation, which has recently been approved, with standardized defined microbial mixtures, and with probiotics, which have been administered with moderate success. Prevention of deleterious effects of antibiotics on microbiota is achieved with agents for the neutralization of antibiotics that act in the gut and are nearing regulatory approval. Neutralization of C. difficile toxins (iii) which are crucial virulence factors is achieved with antibodies/antibody fragments or alternative binding proteins. Of these, the monoclonal antibody bezlotoxumab is already in clinical use. Immunomodulation (iv) can help eliminate or prevent C. difficile infection by interfering with cytokine signaling. Small-molecule agents without bacteriolytic activity are usually selected by drug repurposing and can act via a variety of mechanisms. The multiple treatment options described in this article provide optimism for the future treatment of C. difficile infection. Ključne besede: microbiota, immunomodulators Objavljeno v DiRROS: 17.01.2025; Ogledov: 59; Prenosov: 22 Celotno besedilo (4,12 MB) Gradivo ima več datotek! Več... |
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35. Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesionSpase Stojanov, Tina Vida Plavec, Špela Zupančič, Aleš Berlec, 2024, izvirni znanstveni članek Povzetek: Electrospun nanofibers offer a highly promising platform for the delivery of vaginal lactobacilli, providing an innovative approach to preventing and treating vaginal infections. To advance the application of nanofibers for the delivery of lactobacilli, tools for studying their safety and efficacy in vitro need to be established. In this study, fluorescent (mCherry and GFP) and luminescent (NanoLuc luciferase) proteins were expressed in three vaginal lactobacilli (Lactobacillus crispatus, Lactobacillus gasseri and Lactobacillus jensenii) and a control Lactiplantibacillus plantarum with the aim to use this technology for close tracking of lactobacilli release from nanofibers and their adhesion on epithelial cells. The recombinant proteins influenced the growth of the bacteria, but not their ability to produce hydrogen peroxide. Survival of lactobacilli in nanofibers immediately after electrospinning varied among species. Bacteria retained fluorescence upon incorporation into PEO nanofibers, which was vital for evaluation of their rapid release. In addition, fluorescent labelling facilitated efficient tracking of bacterial adhesion to Caco-2 epithelial cells, while luminescence provided important quantitative insights into bacterial attachment, which varied from 0.5 to 50% depending on the species. The four lactobacilli in dispersion or in nanofibers were not detrimental for the viability of Caco-2 cells, and did not demonstrate hemolytic activity highlighting the safety profiles of both bacteria and PEO nanofibers. To summarize, this study contributes to the development of a promising delivery system, tailored for local administration of safe vaginal lactobacilli. Objavljeno v DiRROS: 17.01.2025; Ogledov: 59; Prenosov: 17 Celotno besedilo (2,68 MB) |
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