1. RNA-dependent RNA polymerase 1 in potato (Solanum tuberosum) and its relationship to other plant RNA-dependent RNA polymerasesLydia J. R. Hunter, Samuel F. Brockington, Alex M. Murphy, Adrienne E. Pate, Kristina Gruden, Stuart A. MacFarlane, Peter Palukaitis, John P. Carr, 2016, izvirni znanstveni članek Povzetek: Cellular RNA-dependent RNA polymerases (RDRs) catalyze synthesis of double-stranded RNAs that can serve to initiate or amplify RNA silencing. Arabidopsis thaliana has six RDR genes; RDRs 1, 2 and 6 have roles in anti-viral RNA silencing. RDR6 is constitutively expressed but RDR1 expression is elevated following plant treatment with defensive phytohormones. RDR1 also contributes to basal virus resistance. RDR1 has been studied in several species including A. thaliana, tobacco (Nicotiana tabacum), N. benthamiana, N. attenuata and tomato (Solanum lycopersicum) but not to our knowledge in potato (S. tuberosum). StRDR1 was identified and shown to be salicylic acid-responsive. StRDR1 transcript accumulation decreased in transgenic potato plants constitutively expressing a hairpin construct and these plants were challenged with three viruses: potato virus Y, potato virus X, and tobacco mosaic virus. Suppression of StRDR1 gene expression did not increase the susceptibility of potato to these viruses. Phylogenetic analysis of RDR genes present in potato and in a range of other plant species identified a new RDR gene family, not present in potato and found only in Rosids (but apparently lost in the Rosid A. thaliana) for which we propose the name RDR7.
Ključne besede: RNA polymerases, RNA, potato
Objavljeno v DiRROS: 25.07.2024; Ogledov: 215; Prenosov: 129
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2. Genetic aspects linked to production and use of forest reproductive material (FRM) : collecting scientific evidence for developing guidelines and decision support tools for effective FRM managementDušan Gömöry, Katri Himanen, Mari Mette Tollefsrud, Claes Uggla, Hojka Kraigher, Sandor Bordacs, Paraskevi Alizoti, Stuart A'Hara, Aline Frank, Gunnar Friis Proschowsky, Josef Frýdl, Thomas Geburek, Monique Guibert, Mladen Ivanković, Ana Jurše, Stuart Kennedy, Jan Kowalczyk, Heike Liesebach, Tiit Maaten, Andrej Pilipović, Roberta Proietti, Volker Schneck, Alain Servais, Brynjar Skúlason, Christoph Sperisen, Frank Wolter, Tefide Yüksel, Michele Bozzano, 2021, slovar, enciklopedija, leksikon, priročnik, atlas, zemljevid Povzetek: This report summarises the current state of knowledge in genetic aspects linked to the production and use of forest reproductive material (FRM). The first chapter describes the different categories of FRM, considers the strategic choice between artificial and natural regeneration in the face of climate change, and articulates the need for and purposes of FRM. Chapter 2 outlines the FRM production chain, in terms of: i) FRM approval, management, collection and certification (seed sources, stands, orchards, plus trees and clones others); ii) Testing standards and examples; iii) Effect of seed and seedling material not intended for use as FRM on forest genetic resources; iv) Breeding effects on basic material including conservation strategy; v) Harvesting, processing and storage of seeds; vi) Nursery practices; vii) Certification and Traceability; and viii) FRM Trading and Transport. Chapter 3 examines FRM with regard to: i) Forest regeneration strategies % especially considering climate change, and ii) Assisted migration and available decision support tools. Chapter 4 provides a detailed overview of forest establishment methods, and chapter 5 looks at keeping records of seed origin to improve forest management. The final chapter offers 38 recommendations arising from the review. This work is the result of an international collaboration rooted in EUFORGEN for more than two decades. It is built on the firm belief that the genetic element is decisive for the creation of a resilient forest capable of surviving threats and adapting to changes, thus enabling the evolution of ecosystems and the conservation of the productive landscape.
Ključne besede: forest reproductive material
Objavljeno v DiRROS: 05.11.2021; Ogledov: 1057; Prenosov: 574
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