1. The physiological impact of GFLV virus infection on grapevine water status : first observationsAnastazija Jež Krebelj, Maja Cigoj, Marija Stele, Marko Chersicola, Maruša Pompe Novak, Paolo Sivilotti, 2022, izvirni znanstveni članek Povzetek: In a vineyard, grapevines are simultaneously exposed to combinations of several abiotic (drought, extreme temperatures, salinity) and biotic stresses (phytoplasmas, viruses, bacteria). With climate change, the incidences of drought in vine growing regions are increased and the host range of pathogens with increased chances of virulent strain development has expanded. Therefore, we studied the impact of the combination of abiotic (drought) and biotic (Grapevine fanleaf virus (GFLV) infection) stress on physiological and molecular responses on the grapevine of cv. Schioppettino by studying the influence of drought and GFLV infection on plant water status of grapevines, on grapevine xylem vessel occlusion, and on expression patterns of 9-cis-epoxycarotenoid dioxygenase 1 (NCED1), 9-cis-epoxycarotenoid dioxygenase 2 (NCED2), WRKY encoding transcription factor (WRKY54) and RD22-like protein (RD22) genes in grapevines. A complex response of grapevine to the combination of drought and GFLV infection was shown, including priming in the case of grapevine water status, net effect in the case of area of occluded vessels in xylem, and different types of interaction of both stresses in the case of expression of four abscisic acid-related genes. Our results showed that mild (but not severe) water stress can be better sustained by GFLV infection rather than by healthy vines. GFLV proved to improve the resilience of the plants to water stress, which is an important outcome to cope with the challenges of global warming. Ključne besede: grapevine, water status, virus infection, GFLV, xylem vessel occlusion, gene expression Objavljeno v DiRROS: 16.07.2024; Ogledov: 109; Prenosov: 110
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2. The physiological impact of GFLV virus infection on grapevine water status : first observationsAnastazija Jež Krebelj, Maja Cigoj, Marija Stele, Marko Chersicola, Maruša Pompe Novak, Paolo Sivilotti, izvirni znanstveni članek Ključne besede: grapevine, water status, virus infection, GFLV, xylem vessel occlusion, gene expression Objavljeno v DiRROS: 06.09.2022; Ogledov: 900; Prenosov: 305
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3. Combined effects of early season leaf removal and climatic conditions on aroma precursors in Sauvignon blanc grapesPaolo Sivilotti, Branka Škvarč, Lorena Butinar, Melita Sternad Lemut, Klemen Lisjak, Andreja Vanzo, 2017, izvirni znanstveni članek Povzetek: Early leaf removal around the cluster zone is a common technique applied in cool climate viticulture, to regulate yield components and improve fruit quality. Despite the increasing amount of information on early leaf removal and its impact on total soluble solids, anthocyanins and polyphenols, less is known regarding aroma compounds. In order to verify the hypothesis that defoliation, applied before or after flowering, could impact the biosynthesis of thiol precursors, we performed a two year (2013 and 2014) experiment on Sauvignon blanc. We provided evidence that differential accumulation of thiols precursors in berries is affected by the timing of defoliation and this impact was related to modifications in biosynthetic pathway. Furthermore, the possible interaction between leaf removal treatment and seasonal weather conditions, and its effect on the biosynthesis of volatile precursors are discussed. Our results suggested that in Sauvignon blanc the relative proportion of 4-S-glutathionyl-4-methylpentan-2-one (G-4MSP) and 3-S-glutathionylhexan-1-ol (G-3SH) precursors can be affected by defoliation, and this could be related to the induction of two specific genes encoding glutathione-S-transferases (VvGST3 and VvGST5), while no significant effects on basic fruit chemical parameters, polyphenols and methoxypyrazines were ascertained under our experimental conditions. Ključne besede: early leaf removal, cluster exposure, thiol precursors, methoxypyrazines, Vitis vinifera Objavljeno v DiRROS: 06.09.2022; Ogledov: 676; Prenosov: 291
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4. Enhancement of Istrian Malvasia wine aroma and hydroxycinnamate composition by hand and mechanical leaf removalMarijan Bubola, Igor Lukič, Sanja Radeka, Paolo Sivilotti, Kristina Grozić, Andreja Vanzo, Dejan Bavčar, Klemen Lisjak, 2019, izvirni znanstveni članek Ključne besede: wine aroma, leaf removal, varietal thiols, aroma compounds, hydroxycinnamates Objavljeno v DiRROS: 11.05.2020; Ogledov: 2465; Prenosov: 1293
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5. Yield reduction through cluster or selective berry thinning similarly modulates anthocyanins and proanthocyanidins composition in Refosco dal peduncolo rosso (Vitis vinifera L.) grapesPaolo Sivilotti, Rachele Falchi, Joshua Vanderweide, Paolo Sabbatini, Marijan Bubola, Andreja Vanzo, Klemen Lisjak, Enrico Peterlunger, Jose Carlos Herrera, 2020, izvirni znanstveni članek Ključne besede: Grapevine, Yield reduction, Selective thinning, Tannins, Anthocyanins, vinska trta, grozdje, pridelek, redukcija pridelka, redčenje, tanini, antociani, kemična sestava Objavljeno v DiRROS: 06.05.2020; Ogledov: 2245; Prenosov: 314
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