1. Validating the potential of double-stranded RNA targeting Colorado potato beetle mesh gene in laboratory and field trialsMarko Petek, Anna Coll Rius, Rok Ferenc, Jaka Razinger, Kristina Gruden, 2020, izvirni znanstveni članek Povzetek: Colorado potato beetle (CPB) is an agricultural pest of solanaceous crops, notorious for its rapid resistance development to chemical pesticides. Foliar spraying of dsRNA formulations is a promising innovative technology providing highly specific and environmentally acceptable option for CPB management. We designed dsRNA to silence CPB mesh gene (dsMESH) and performed laboratory feeding trials to assess impacts on beetle survival and development. We compared the effectiveness of in vivo and in vitro produced dsRNA in a series of laboratory experiments. We additionally performed a field trial in which the efficacy of dsRNA sprayed onto potato foliage was compared to a spinosad-based insecticide. We showed that dsMESH ingestion consistently and significantly impaired larval growth and decreased larval survival in laboratory feeding experiments. In vivo produced dsRNA performed similarly as in vitro synthesized dsRNA in laboratory settings. In the field trial, dsMESH was as effective in controlling CPB larvae as a commercial spinosad insecticide, its activity was however slower. We discuss limitations and benefits of a potential dsMESH-based CPB management strategy and list some important RNAi based CPB research topics, which will have to be addressed in future. Ključne besede: RNA interference, RNA interference feeding, ds RNA, gene silencing, RNAi pest control, survival analysis, field trial Objavljeno v DiRROS: 22.07.2024; Ogledov: 136; Prenosov: 51
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2. Effect of belowground deployment of entomopathogenic fungus Metarhizium brunneum on strawberry plants and aboveground pestsJaka Razinger, Darinka Koron, Špela Modic, Eva Praprotnik, Primož Žigon, Uroš Žibrat, Matej Knapič, Janez Lapajne, Andrej Vončina, Lovro Sinkovič, Nika Cvelbar Weber, 2023, drugi znanstveni članki Ključne besede: biological control, integrated pest management, agricultural entomology, entomopathogenic fungi, hyperspectral imaging, remote sensing, plant-microbe-pest interaction, plant physiology, rhizosphere competence Objavljeno v DiRROS: 27.07.2023; Ogledov: 549; Prenosov: 0 |
3. Potential of cover crops to control arthropod pests in organic viticultureAurora Ranca, Anne-Laure Fragnière, Sergiu Ayar Ene, Špela Modic, Vittorio Rossi, Tito Caffi, Jaka Razinger, Eugenia Rodrigo Santamalia, Daniel Wipf, Patrik Kehrli, izvirni znanstveni članek Ključne besede: conservation biological control, pest control, functional diversity Objavljeno v DiRROS: 06.09.2022; Ogledov: 653; Prenosov: 318
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4. Agricultural landscape affects sex-specific differences in the abundance of Drosophila suzukii in raspberry orchardsMaarten De Groot, Magda Rak Cizej, Andreja Kavčič, Špela Modic, Franček Poličnik, Nina Šramel, Primož Žigon, Jaka Razinger, 2022, izvirni znanstveni članek Povzetek: In recent decades, the spotted wing Drosophila (SWD) (Drosophila suzukii), an invasive pest, has caused a great deal of damage to fruit crops. There is therefore an urgent need to develop strategies to control the populations of this species. It has been found that the landscape context can buffer or increase the severity of pest outbreaks in agriculture, and it is important to understand how this process works in SWD for all crops. Given this background, we investigated the influence of forest on SWD populations in raspberry orchards and surrounding agricultural land. We selected 10 locations in the central part of Slovenia, five of which were closer than 200 m from the forest edge and five of which were more than 200 m from the forest edge. We collected SWD adults in three habitat types per location from the end of June until the end of October 2020. The results showed that forest harboured a larger SWD population than orchards and agricultural land. Over the season, the number of individuals increased exponentially over time, and the difference between forest and other habitat types increased. The distance from the forest had a negative effect on the abundance of SWD. There was a difference in abundance observed between males and females, with males being less abundant farther away from the forest than females. However, the distance from the forest only had a negative effect on the abundance of females in September. Based on the results, we propose potential measures for the control of SWD in raspberry orchards. Ključne besede: agricultural ecosystem, integrated pest management, invasive species, raspberry, spotted wing drosophila Objavljeno v DiRROS: 15.11.2021; Ogledov: 912; Prenosov: 905
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5. Confirmed and potential wild hosts of the Spotted wing drosophila (Drosophila suzukii) in SloveniaMaarten De Groot, Andreja Kavčič, Jaka Razinger, 2020, izvirni znanstveni članek Povzetek: The spotted wing drosophila (Drosophila suzukii (Matsumura)) is a highly invasive species and attacking different species of berry carrying hosts. Much research has already been done on the crop hosts over the world and in Slovenia, but for wild hosts less is known. On basis of literature and fieldwork we prepared a list of potential and actual known species of wild hosts for Slovenia. In 2019, berries of different species were collected and D. suzukii was either reared from these berries or berries were dissected. In total we found in the literature for Europe 99 species which were used as host for D. suzukii. For Slovenia we found 71 potential hosts and 14 hosts which were actually infested. in Slovenia there was a broad range of potential hosts from 41 genera. The genera with the most potential hosts were Prunus, Lonicera and Vaccinium. Among the potential hosts were also many species which were invasive alien or alien species. The list was discussed in the context of management implications and further research on D. suzukii in Slovenia. Ključne besede: spotted wing drosophila, berries, potential hosts, wild hosts, forests, pest control, Slovenia Objavljeno v DiRROS: 11.12.2020; Ogledov: 1375; Prenosov: 617
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7. Discrimination between abiotic and biotic drought stress in tomatoes using hyperspectral imagingNik Susič, Uroš Žibrat, Saša Širca, Polona Strajnar, Jaka Razinger, Matej Knapič, Andrej Vončina, Gregor Urek, Barbara Gerič Stare, izvirni znanstveni članek Povzetek: Crop plants are subjected to various biotic and abiotic stresses. Both root-knot nematodes (biotic stress) and water deficiency (abiotic stress) lead to similar drought symptoms in the plant canopy. In this work, hyperspectral imaging was used for early detection of nematode infestation and water deficiency (drought) stress in tomato plants. Hyperspectral data in the range from 400 to 2500 nm of plants subjected to different watering regimes and nematode infestation levels were analysed by partial least squares – discriminant analysis (PLS-DA) and partial least squares – support vector machine (PLS-SVM) classification. PLS-SVM classification achieved up to 100% accuracy differentiating between well-watered and water-deficient plants, and between 90 and 100% when identifying nematode-infested plants. Grouping the data according to the time of imaging increased the accuracy of classification. Shortwave infrared spectral regions associated with the OH and CH stretches were most relevant for the identification of nematode infested plants and severity of infestation. This study demonstrates the capability of hyperspectral imaging to identify and discriminate between biotic and abiotic plant stresses. Ključne besede: nematode, Hyperspectral imaging, Drought stress, Root-knot nematode, Tomato Objavljeno v DiRROS: 20.07.2018; Ogledov: 4108; Prenosov: 2558
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