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Povzetek: Rapid and accurate identification of bacterial pathogens is crucial for effective treatment and infection control, particularly in hospital settings. Conventional methods like culture techniques and MALDI-TOF mass spectrometry are often time-consuming and less sensitive. This study addresses the need for faster and more precise diagnostic methods by developing novel digital PCR (dPCR) assays for the rapid quantification of biomarkers from three Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Utilizing publicly available genomes and the rapid identification of PCR primers for unique core sequences or RUCS algorithm, we designed highly specific dPCR assays. These assays were validated using synthetic DNA, bacterial genomic DNA, and DNA extracted from clinical samples. The developed dPCR methods demonstrated wide linearity, a low limit of detection (approx. 30 copies per reaction), and robust analytical performance with measurement uncertainty below 25 %. The assays showed high repeatability and intermediate precision, with no cross-reactivity observed. Comparison with MALDI-TOF mass spectrometry revealed substantial concordance, highlighting the methods’ suitability for clinical diagnostics. This study underscores the potential of dPCR for rapid and precise quantification of Gram-negative bacterial biomarkers. The developed methods offer significant improvements over existing techniques, providing faster, more accurate, and SI-traceable measurements. These advancements could enhance clinical diagnostics and infection control practices.
Ključne besede: digital PCR (dPCR), Gram-negative bacteria, pathogen detection, respiratory infections, biomarkers, RUCS algorithm
Objavljeno v DiRROS: 05.11.2024; Ogledov: 170; Prenosov: 347
Celotno besedilo (2,31 MB)
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Povzetek: This article addresses the need to adopt an intersectional perspective when discussing sexual harassment and violence in academia. The introduction of an intersectional perspective is important since sexual harassment and violence in relation to racial and ethnic minorities seldom revolve exclusively around gender. Recognising the intersectional nature of sexual assault and harassment means also recognising the coexistence of racism and nationalism, the perpetuation of ethnic/racial stereotypes, the multifaceted manifestations of discrimination, and a matrix of domination that exists in our society and educational and research institutions as well. In this article, we analysed official documents of Slovenian higher education and research institutions (public and private ones) with an aim to examine whether and how they address intersectionality and racial/ethnic minorities in their policies combating sexual harassment and violence. The results revealed a significant ignorance towards intersectionality of gender and ethnicity, but also issues arising from accessibility of these policies.
Ključne besede: sexual harassment and violence, intersectionality, gender, “race”, ethnicity, university, research institutions
Objavljeno v DiRROS: 30.10.2024; Ogledov: 236; Prenosov: 125
Celotno besedilo (1,28 MB)
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Povzetek: Red mud (RM) is an extractive waste from Bayer process in alumina production. The most conventional methods of RM disposal are tailing dams, or dry storage,both of which raiseenvironmental concerns due to the high alkalinity of RMandthe presence and leaching of potentially hazardous elements (PTEs).Due to its fine-grained nature and poor mechanical properties, as well as leaching of potentially hazardous substances, RM cannot be used by itself. Properties of the RM can be improved, by mixing it with hydraulic or pozzolanic binders, such as calcareous ashes or steelmaking slag, to produce mechanically stable and durable composites for earthworks. On the other hand, RMcan also serve as an immobilization additive for remediation of contaminated soils.Two different approaches for useful utilization of RMare presented based on results of laboratory research and a real case study, opening of new possibilities for conserving natural resources and reducing environmental hazards of RM deposits.
Ključne besede: red mud, recycling, geotechnical composites, environment
Objavljeno v DiRROS: 30.10.2024; Ogledov: 272; Prenosov: 829
Celotno besedilo (8,18 MB)
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Ključne besede: gozdovi, lesno oglje, primerjava, žar, analiza kakovosti, trg, Slovenija
Objavljeno v DiRROS: 29.10.2024; Ogledov: 200; Prenosov: 781
Celotno besedilo (1,68 MB)

Ključne besede: Weed germination, Dormancy, Persistence, Tillage practice, Weed diversity, Species richness
Objavljeno v DiRROS: 29.10.2024; Ogledov: 223; Prenosov: 337
Celotno besedilo (3,81 MB)