51. Carboxypeptidase cathepsin X defines a multifunctional role of gamma-enolase in cancerTjaša Vižin, Anja Pišlar, Ib Jarle Christensen, Hans Jørgen Nielsen, Pika Meško-Brguljan, Janko Kos, 2022, izvirni znanstveni članek Povzetek: Gamma-enolase enzymatic activity is involved in glycolysis, a prevalent process in cancer cell metabolism. Additionally, gamma-enolase has a pro-survival function, exhibited through the active site at the C-terminal end of the molecule. This activity is regulated by cysteine peptidase cathepsin X, which cleaves two amino acids at C-terminal end of gamma-enolase. In clinical practice, the determination of gamma-enolase as a tumour marker does not differ between total, uncleaved and C-terminally cleaved forms. However, levels of uncleaved gamma-enolase alone may provide additional clinical information. In this study we analysed cathepsin X, C- terminally uncleaved and total gamma-enolase in tumour cell lines and sera from 255 patients with colorectal cancer (CRC) by western blot, immunoprecipitation, enzymatic activity, ELISAs and ECLIA. Results show that uncleaved gamma-enolase, rather than total gamma- enolase, exhibits different levels in cells, being the highest in those, derived from metastatic sites or highly invasive tumours. Gamma-enolase is secreted into the extracellular space predominantly as an uncleaved form and levels were congruent to those within the cells. Furthermore, levels of uncleaved gamma-enolase in cells are inversely related to cathepsin X protein level and its enzymatic activity. Uncleaved gamma-enolase is also predominant form in sera of patients with CRC. Both forms exhibit similar stage dependent distribution, with slightly elevated levels in stage IV patients. Higher levels of total gamma-enolase are significantly related to shorter survival in patients with metastatic CRC. Results support evidence of additional pro-survival function of gamma-enolase in cancer. Future studies should focus on analysis of uncleaved gamma-enolase in tumour samples, which may provide additional relations to clinical indicators of disease progression.
Ključne besede: cancer, cathepsin X, cell survival, gamma-enolase, prognosis
Objavljeno v DiRROS: 06.04.2022; Ogledov: 860; Prenosov: 478
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52. Poudarki dialoga med kristjani in marksisti na Slovenskem v 80. letih 20. stoletjaAnja Zalta, 2020, izvirni znanstveni članek Povzetek: Prispevek izpostavi (nekatere) poudarke dialoga med t. i. marksisti in katoličani v 80. letih 20. stoletja na Slovenskem. Osrednja os razprave se vrti okoli (razumevanja) metode historičnega materializma in naglaševanja marksizma kot svetovnega nazora. Na podlagi predstavitve zgodovinskega ozadja, predvsem odnosa Cerkve in papeških stališč do socializma in posledično do marksizma in komunizma, ter vprašanja rekatolizacije, se prispevek posveti teološkim odzivom na historični materializem. Izhajajoč iz konteksta protikomunistične restavracije ostaja posebej problematična retorika "kulturnega boja" kot spopada med dvema svetovnima nazoroma, ki posledično prinaša in utrjuje ideje, da gre pri marksizmu in (katoliški) religioznosti prav za takšen spopad. Članek problematizira tovrstne pozicije, hkrati pa pokaže na pomen vpeljave metode historičnega materializma, ki jo je (do neke mere) posvojila socialistična teologija mariborskega pomožnega škofa Vekoslava Grmiča in njegovega kroga.
Ključne besede: marksizem, katolicizem, socialistična teologija, historični materializem, Slovenija
Objavljeno v DiRROS: 28.03.2022; Ogledov: 575; Prenosov: 362
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56. Pesticidi v vodonosniku Krško-Brežiškega poljaNina Mali, Anja Koroša, Janko Urbanc, 2021, izvirni znanstveni članek Povzetek: Onesnaženje podzemne vode s pesticidi je splošno razširjen problem, tako v svetu kot tudi v Sloveniji. Glede na pretekle velike obremenitve podzemne vode s pesticidi, je bil namen predstavljene raziskave ugotoviti razširjenost pesticidov v podzemni vodi Krško-Brežiškega polja v obdobju 2018 - 2019 in pri tem preveriti uporabnost metode vzorčenja vode s pasivnimi vzorčevalniki. Skupno smo odvzeli 21 vzorcev podzemne vode na enajstih lokacijah in po dva vzorca v rekah Sava in Krka. V vodi smo določili 15 različnih pesticidov in njihovih razgradnih produktov. V vzorcih podzemne vode sta bila največkrat določena atrazin in njegov razgradni produkt desetilatrazin. Sledijo pesticidi desetilterbutilazin, terbutilazin, metolaklor ter simazin. V površinski vodi smo zaznali atrazin, desetilatrazin, klortoluron, metolaklor in terbutilazin. S kvalitativno metodo vzorčenja s pasivnimi vzorčevalniki smo v podzemni in površinski vodi odvzeli skupno 24 vzorcev. Izločili smo 8 pesticidov, ki se pojavljajo v dveh serijah. Pogostnost pojavljanja posameznih pesticidov je po obeh metodah primerljiva. Pasivno vzorčenje vode se je izkazalo za primerno metodo identifikacije prisotnosti pesticidov. Največje obremenitve s pesticidi na Krško-Brežiškem polju prihajajo s kmetijskih površin. Podzemna voda je bolj obremenjena s pesticidi v osrednjem delu polja v smeri toka od zahoda proti vzhodu. Atrazin in desetilatrazin sta še vedno, kljub dvajsetletni prepovedi, najpogosteje in v najvišjih koncentracijah zaznana pesticida v podzemni vodi Krško-Brežiškega polja.
Ključne besede: podzemna voda, vodonosnik Krško-Brežiško polje, pesticidi
Objavljeno v DiRROS: 09.03.2022; Ogledov: 868; Prenosov: 329
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57. Geophysical investigations in the Radovna River Spring area (Julian Alps, NW Slovenia)Anja Torkar, Marjana Zajc, Jure Atanackov, Andrej Gosar, Mihael Brenčič, 2021, izvirni znanstveni članek Povzetek: The Radovna River Valley is located in the north-western part of Slovenia in the Julian Alps, where there is an extensive intergranular aquifer whose depth to pre-Quaternary bedrock is unknown. Therefore, to obtain information about the depth of the valley and the geometry of the aquif er two geophysical methods were used in our study; ground penetrating radar (GPR) and seismic reflection method. The low-frequency GPR method has shown to be useful for determining the depth of the groundwater and the predominant groundwater recharge. Also, the high-resolution seismic method provided an insight about the morphology of the pre-Quaternary basement with the deepest point at 141 meters below surface. Measurements of hydrogeological parameters such as groundwater level and river discharge measurements were carried out in the study area. Both data analyses showed that groundwater level and river discharge are highly fluctuatingand rapidly changing, indicating a well-permeable aquifer, implying that such an aquifer is extremely sensitive and vulnerable to extreme climate events. Both the geophysical methods and the hydrogeological information have provided important information about the morphology of the valley and the alluvial aquifer, as well as increasing the knowledge about the Radovna springs system, which will contribute very important information for future hydrogeological studies.
Ključne besede: Ground penetrating radar, Seismic reflection method, Radovna spring, hydrogeology, aquifer geometry, glacial valley, groundwater table
Objavljeno v DiRROS: 09.03.2022; Ogledov: 774; Prenosov: 326
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58. Sedimentological and paleontological analysis of the Lower Jurassic part of the Zatrnik Formation on the Pokljuka plateau, SloveniaLuka Gale, Duje Kukoč, Boštjan Rožič, Anja Vidervol, 2021, izvirni znanstveni članek Povzetek: The uppermost Ladinian to Lower Jurassic Zatrnik Formation is the lithostratigraphic unit of the Mesozoic deeper marine Bled Basin. The uppermost part of the Zatrnik Formation and the transition into the overlying Ribnica Breccia was logged at the Zajamniki mountain pasture on the Pokljuka mountain plateau in the Julian Alps. The lowermost part the section belongs to the “classical” Zatrnik Formation and is dominated by beige micritic limestone and fine-grained calcarenite. Foraminifers Siphovalvulina, ?Everticyclammina, ?Mesoendothyra and ?Pseudopfenderina are present, indicating Early Jurassic age. The beige limestone is followed by light pink limestone of the uppermost Zatrnik Formation. Slumps are common in this interval, and crinoids are abundant. Alongside some species already present in beds lower in the succession, Meandrovoluta asiagoensis Fugagnoli & Rettori, Trocholina sp., Valvulinidae, small Textulariidae, Lagenida, and small ?Ophthalmidium alsooccur in this interval. Resedimented limestone predominates through the studied part of the Zatrnik Formation, indicating deposition on the slope or at the foot of the slope of the basin. The switch to crinoid-rich facies within the slumped interval of the Zatrnik Formation may reflect accelerated subsidence of the margins of the Julian Carbonate Platform in the Pliensbachian. The Zatrnik Formation is followed by the formation of the Pliensbachian (?) Ribnica Breccia. Impregnations of ferromanganese oxides, violet colour, and an increase in clay content are characteristic. The foraminiferal assemblage consists of Lenticulina, small elongated Lagenida, and epistominids. Individual beds of the Ribnica Breccia were deposited via debris flows. Enrichments in ferromanganese oxides point to slower sedimentation.
Ključne besede: Lower Jurassic, Bled Basin, Pokljuka Nappe, stratigraphy, foraminifer, Hierlatz facies, Ribnica Breccia, Zajamniki
Objavljeno v DiRROS: 09.03.2022; Ogledov: 735; Prenosov: 305
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59. Passive sampling with active carbon fibres in the determination of organic pollutants in groundwaterPrimož Auersperger, Anja Koroša, Nina Mali, Brigita Jamnik, 2022, izvirni znanstveni članek Povzetek: Legislation addressing the quality of groundwater and increasing concerns over public health calls for the development of analytical methods that can produce accurate and precise results at the ppt level. Passive sampling has been recognised as a helpful tool in identifying various organic pollutants in groundwater, even when their presence had not yet been identified through conventional groundwater quality monitoring. The article presents an analytical method involving a simple and cost-effective passive sampling device using Zorflex® activated carbon fibres (ACFs) for the qualitative monitoring of a broad range of organic pollutants in water in a single run. The applicability of the method developed was tested in three hydrogeological studies. In the first case, we present a non-targeted qualitative screening and a list of 892 different contaminants detected in the groundwater in Slovenia. In the second case, we discuss the presence and origin of organic compounds in the groundwater from a pilot area of the urban aquifer, Ljubljansko polje. The third case presents a comparison of results between passive and grab sampling. Passive sampling with ACFs confirmed the presence of a pollutant, even when it had not been previously detected through a quantitative method.
Ključne besede: GC-MS, organic pollutants, qualitative analysis, passive sampling, groundwater
Objavljeno v DiRROS: 03.03.2022; Ogledov: 746; Prenosov: 306
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60. Zdravljenje intrahepatalnega holangiokarcinoma s stereotaktičnim obsevanjem : prikaz primeraAnja Meden Boltežar, Ajra Šečerov Ermenc, 2021, strokovni članek Povzetek: V prispevku je opisan primer bolnice z lokalno napredovalim holangiokarcinomom intrahepatalnih žolčnih vodov, ki je redek malignom s svojevrstnim kliničnim potekom. Ob postavitvi diagnoze ima večina bolnikov napredovalo obliko bolezni in so posledično kandidati le za nekirurško zdravljenje – kemoterapijo, kombinacijo kemoterapije in radioterapije ali samo radioterapijo. Glavna omejitev pri zdravljenju z radioterapijo je doza, ki jo prejmejo jetra in priležni cevasti organi, zato se v zadnjem času čedalje bolj uveljavlja zdravljenje s stereotaktično radioterapijo. Ta omogoča, da dovedemo visoko dozo na tumor, pri čemer ščitimo rizične organe. Raziskave, ki so bile objavljene v zadnjih letih, so pokazale dobre rezultate zdravljenja inoperabilne bolezni z namenom poskusa zazdravitve bolezni in podaljšanja celokupnega preživetja.
Ključne besede: intrahepatalni holangiokarcinom, stereotaktično obsevanje, radioterapija
Objavljeno v DiRROS: 11.06.2021; Ogledov: 2194; Prenosov: 422
Celotno besedilo (554,08 KB) |
