1. Genotoxic effects of cylindrospermopsin, microcystin-LR and their binary mixture in human hepatocellular carcinoma (HepG2) cell lineLeticia Díez-Quijada, Klara Hercog, Martina Štampar, Metka Filipič, Ana M. Cameán, Angeles Jos, Bojana Žegura, 2020, izvirni znanstveni članek Povzetek: Simultaneous occurrence of cylindrospermopsin (CYN) and microcystin-LR (MCLR) has been reported in the aquatic environment and thus human exposure to such mixtures is possible. As data on the combined effects of CYN/MCLR are scarce, we aimed to investigate the adverse effects related to genotoxic activities induced by CYN (0.125, 0.25 and 0.5 µg/mL) and MCLR (1 µg/mL) as single compounds and their combinations in HepG2 cells after 24 and 72 h exposure. CYN and CYN/MCLR induced DNA double-strand breaks after 72 h exposure, while cell cycle analysis revealed that CYN and CYN/MCLR arrested HepG2 cells in G0/G1 phase. Moreover, CYN and the combination with MCLR upregulated CYP1A1 and target genes involved in DNA-damage response (CDKN1A, GADD45A). Altogether, the results showed that after 72 h exposure genotoxic activity of CYN/MCLR mixture was comparable to the one of pure CYN. On the contrary, MCLR (1 µg/mL) had no effect on the viability of cells and had no influence on cell division. It did not induce DNA damage and did not deregulate studied genes after prolonged exposure. The outcomes of the study confirm the importance of investigating the combined effects of several toxins as the effects can differ from those induced by single compounds.
Objavljeno v DiRROS: 23.07.2024; Ogledov: 113; Prenosov: 121
 Celotno besedilo (1,68 MB)
Gradivo ima več datotek! Več... |
2. Hay meadow vibroscape and interactions within insect vibrational communityRok Šturm, Behare Rexhepi, Juan José López Díez, Andrej Blejec, Jernej Polajnar, Jérôme Sueur, Meta Virant-Doberlet, 2021, izvirni znanstveni članek Povzetek: Our experiences shape our knowledge and understanding of the world around us. The natural vibrational environment (vibroscape) is hidden to human senses but is nevertheless perceived and exploited by the majority of animals. Here, we show that the vibroscape recorded on plants in a temperate hay meadow is a dynamic low-frequency world, rich in species-specific vibrational signals. The overall vibroscape composition changed throughout the season and also depended on the plant species, as well as on the spatial position of individual plants within the meadow. Within the studied community, vibrationally signaling species sharing this communication channel avoided interference primarily by partitioning vibrational space on a fine temporal scale. The vibroscape is a reliable source of information in the environment and expands our understanding of ecological and evolutionary processes.
Objavljeno v DiRROS: 19.07.2024; Ogledov: 132; Prenosov: 134
Celotno besedilo (4,65 MB)
Gradivo ima več datotek! Več... |
3. Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymesLenny Ferrer, Melanie Mindt, Maria Suarez-Diez, Tatjana Jilg, Maja Zagorščak, Jin-Ho Lee, Kristina Gruden, Volker F. Wendisch, Katarina Cankar, 2022, izvirni znanstveni članek Povzetek: Indole is produced in nature by diverse organisms and exhibits a characteristic odor described as animal, fecal, and floral. In addition, it contributes to the flavor in foods, and it is applied in the fragrance and flavor industry. In nature, indole is synthesized either from tryptophan by bacterial tryptophanases (TNAs) or from indole-3-glycerol phosphate (IGP) by plant indole-3-glycerol phosphate lyases (IGLs). While it is widely accepted that the tryptophan synthase α-subunit (TSA) has intrinsically low IGL activity in the absence of the tryptophan synthase β-subunit, in this study, we show that Corynebacterium glutamicum TSA functions as a bona fide IGL and can support fermentative indole production in strains providing IGP. By bioprospecting additional bacterial TSAs and plant IGLs that function as bona fide IGLs were identified. Capturing indole in an overlay enabled indole production to titers of about 0.7 g L–1 in fermentations using C. glutamicum strains expressing either the endogenous TSA gene or the IGL gene from wheat.
Ključne besede: Corynebacterium glutamicum, indole, indole-3-glycerol phosphate lyase, tryptophan synthase α-subunit, bioprospecting, fermentative production
Objavljeno v DiRROS: 16.07.2024; Ogledov: 158; Prenosov: 147
Celotno besedilo (2,24 MB)
Gradivo ima več datotek! Več... |
4. Is it time for ecotremology?Rok Šturm, Juan José López Díez, Jernej Polajnar, Jérôme Sueur, Meta Virant-Doberlet, 2022, izvirni znanstveni članek Povzetek: Our awareness of air-borne sounds in natural and urban habitats has led to the recent recognition of soundscape ecology and ecoacoustics as interdisciplinary fields of research that can help us better understand ecological processes and ecosystem dynamics. Because the vibroscape (i.e., the substrate-borne vibrations occurring in a given environment) is hidden to the human senses, we have largely overlooked its ecological significance. Substrate vibrations provide information crucial to the reproduction and survival of most animals, especially arthropods, which are essential to ecosystem functioning. Thus, vibroscape is an important component of the environment perceived by the majority of animals. Nowadays, when the environment is rapidly changing due to human activities, climate change, and invasive species, this hidden vibratory world is also likely to change without our notice, with potentially crucial effects on arthropod communities. Here, we introduce ecotremology, a discipline that mainly aims at studying substrate-borne vibrations for unraveling ecological processes and biological conservation. As biotremology follows the main research concepts of bioacoustics, ecotremology is consistent with the paradigms of ecoacoustics. We argue that information extracted from substrate vibrations present in the environment can be used to comprehensively assess and reliably predict ecosystem changes. We identify key research questions and discuss the technical challenges associated with ecotremology studies.
Ključne besede: ecotremology, monitoring, hidden biodiversity, vibroscape, vibrational communication
Objavljeno v DiRROS: 16.07.2024; Ogledov: 131; Prenosov: 118
Celotno besedilo (3,92 MB)
Gradivo ima več datotek! Več... |
5. Atmosphere - vegetation - soil interactions in a climate change context; impact of changing conditions on engineered transport infrastructure slopes in EuropeAnh Minh Tang, P. N. Hughes, T. A. Dijkstra, Amin Askarinejad, Mihael Brenčič, Yu Jun Cui, J. J. Diez, T. Firgi, Beata Gajewska, F. Gentile, G. Grossi, C. Jommi, F. Kehagia, E. Koda, H. W. ter Maat, Stanislav Lenart, S. Lourenco, M. Oliveira, P. Osinski, Sarah Springman, Ross Stirling, D. G. Toll, Ursula J. Van Beek, 2018, izvirni znanstveni članek Povzetek: In assessing the impact of climate change on infrastructure, it is essential to consider the interactions between the atmosphere, vegetation and the near-surface soil. This paper presents an overview of these processes, focusing on recent advances from the literature and those made by members of COST Action TU1202 - Impacts of climate change on engineered slopes for infrastructure. Climate- and vegetation-driven processes (suction generation, erosion, desiccation cracking, freeze-thaw effects) are expected to change in incidence and severity, which will affect the stability of new and existing infrastructure slopes. This paper identifies the climate- and vegetation-driven processes that are of greatest concern, the suite of known unknowns that require further research, and lists key aspect that should be considered for the design of engineered transport infrastructure slopes in the context of climate change.
Objavljeno v DiRROS: 12.12.2023; Ogledov: 295; Prenosov: 173
Celotno besedilo (2,29 MB)
Gradivo ima več datotek! Več... |
